DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ brooks-2007-1 mouse Mus musculus Mouse anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. DIP original experiment: DIP-261743X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Bak regulates mitochondrial morphology and pathology during apoptosis by interacting with mitofusins. Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. Only protein-protein interactions imex curation brooks-2007-2 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. DIP original experiment: DIP-261739X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Bak regulates mitochondrial morphology and pathology during apoptosis by interacting with mitofusins. Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. Only protein-protein interactions imex curation brooks-2007-3 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. DIP original experiment: DIP-261741X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Bak regulates mitochondrial morphology and pathology during apoptosis by interacting with mitofusins. Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. Only protein-protein interactions imex curation brooks-2007-4 human Homo sapiens Human fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. DIP original experiment: DIP-261745X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Bak regulates mitochondrial morphology and pathology during apoptosis by interacting with mitofusins. Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. Only protein-protein interactions imex curation brooks-2007-5 human Homo sapiens Human fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. DIP original experiment: DIP-261747X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Bak regulates mitochondrial morphology and pathology during apoptosis by interacting with mitofusins. Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. Only protein-protein interactions imex curation brooks-2007-6 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. DIP original experiment: DIP-261740X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Bak regulates mitochondrial morphology and pathology during apoptosis by interacting with mitofusins. Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. Only protein-protein interactions imex curation brooks-2007-7 mouse Mus musculus Mouse anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. DIP original experiment: DIP-261742X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Bak regulates mitochondrial morphology and pathology during apoptosis by interacting with mitofusins. Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. Only protein-protein interactions imex curation brooks-2007-8 mouse Mus musculus Mouse anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. DIP original experiment: DIP-261744X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Bak regulates mitochondrial morphology and pathology during apoptosis by interacting with mitofusins. Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. Only protein-protein interactions imex curation brooks-2007-9 human Homo sapiens Human fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. DIP original experiment: DIP-261746X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Bak regulates mitochondrial morphology and pathology during apoptosis by interacting with mitofusins. Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. Only protein-protein interactions imex curation brooks-2007-10 human Homo sapiens Human fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. DIP original experiment: DIP-261748X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Bak regulates mitochondrial morphology and pathology during apoptosis by interacting with mitofusins. Brooks C., Wei Q., Feng L., Dong G., Tao Y., Mei L., Xie ZJ., Dong Z. Only protein-protein interactions imex curation mfn2_mouse Mitofusin-2 Mfn2 Kiaa0214 Marf Hypertension-related protein 1 Mitochondrial assembly regulatory factor Transmembrane GTPase MFN2 protein protein mouse Mus musculus Mouse MSLLFSRCNSIVTVKKDKRHMAEVNASPLKHFVTAKKKINGIFEQLGAYIQESASFLEDTHRNTELDPVTTEEQVLDVKGYLSKVRGISEVLARRHMKVAFFGRTSNGKSTVINAMLWDKVLPSGIGHTTNCFLRVGGTDGHEAFLLTEGSEEKKSVKTVNQLAHALHQDEQLHAGSMVSVMWPNSKCPLLKDDLVLMDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKQFFHKVSERLSRPNIFILNNRWDASASEPEYMEEVRRQHMERCTSFLVDELGVVDRAQAGDRIFFVSAKEVLSARVQKAQGMPEGGGALAEGFQVRMFEFQNFERQFEECISQSAVKTKFEQHTVRAKQIAEAVRLIMDSLHIAAQEQRVYCLEMREERQDRLRFIDKQLELLAQDYKLRIKQITEEVERQVSTAMAEEIRRLSVLVDEYQMDFHPSPVVLKVYKNELHRHIEEGLGRNLSDRCSTAIASSLQTMQQDMIDGLKPLLPVSMRNQIDMLVPRQCFSLSYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALLGYSDQVQRPLPLTPANPSMPPLPQSSLTQEELMVSMVTGLASLTSRTSMGILVVGGVVWKAVGWRLIALSFGLYGLLYVYERLTWTTKAKERAFKRQFVEYASEKLQLIISYTGSNCSHQVQQELSGTFAHLCQQVDITRDNLEQEIAAMNKKVEALDSLQSRAKLLRNKAGWLDSELNMFTHQYLQPSR mint bak_mouse Bcl-2 homologous antagonist/killer Apoptosis regulator BAK Bak1 Bak protein protein mouse Mus musculus Mouse MASGQGPGPPKVGCDESPSPSEQQVAQDTEEVFRSYVFYLHQQEQETQGAAAPANPEMDNLPLEPNSILGQVGRQLALIGDDINRRYDTEFQNLLEQLQPTAGNAYELFTKIASSLFKSGISWGRVVALLGFGYRLALYVYQRGLTGFLGQVTCFLADIILHHYIARWIAQRGGWVAALNFRRDPILTVMVIFGVVLLGQFVVHRFFRS mfn2_human Mitofusin-2 MFN2 CPRP1 KIAA0214 Transmembrane GTPase MFN2 protein protein human Homo sapiens Human MSLLFSRCNSIVTVKKNKRHMAEVNASPLKHFVTAKKKINGIFEQLGAYIQESATFLEDTYRNAELDPVTTEEQVLDVKGYLSKVRGISEVLARRHMKVAFFGRTSNGKSTVINAMLWDKVLPSGIGHTTNCFLRVEGTDGHEAFLLTEGSEEKRSAKTVNQLAHALHQDKQLHAGSLVSVMWPNSKCPLLKDDLVLMDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKHFFHKVSERLSRPNIFILNNRWDASASEPEYMEEVRRQHMERCTSFLVDELGVVDRSQAGDRIFFVSAKEVLNARIQKAQGMPEGGGALAEGFQVRMFEFQNFERRFEECISQSAVKTKFEQHTVRAKQIAEAVRLIMDSLHMAAREQQVYCEEMREERQDRLKFIDKQLELLAQDYKLRIKQITEEVERQVSTAMAEEIRRLSVLVDDYQMDFHPSPVVLKVYKNELHRHIEEGLGRNMSDRCSTAITNSLQTMQQDMIDGLKPLLPVSVRSQIDMLVPRQCFSLNYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALMGYNDQVQRPIPLTPANPSMPPLPQGSLTQEEFMVSMVTGLASLTSRTSMGILVVGGVVWKAVGWRLIALSFGLYGLLYVYERLTWTTKAKERAFKRQFVEHASEKLQLVISYTGSNCSHQVQQELSGTFAHLCQQVDVTRENLEQEIAAMNKKIEVLDSLQSKAKLLRNKAGWLDSELNMFTHQYLQPSR mfn1_human Mitofusin-1 Transmembrane GTPase MFN1 Fzo homolog MFN1 protein protein human Homo sapiens Human MAEPVSPLKHFVLAKKAITAIFDQLLEFVTEGSHFVEATYKNPELDRIATEDDLVEMQGYKDKLSIIGEVLSRRHMKVAFFGRTSSGKSSVINAMLWDKVLPSGIGHITNCFLSVEGTDGDKAYLMTEGSDEKKSVKTVNQLAHALHMDKDLKAGCLVRVFWPKAKCALLRDDLVLVDSPGTDVTTELDSWIDKFCLDADVFVLVANSESTLMNTEKHFFHKVNERLSKPNIFILNNRWDASASEPEYMEDVRRQHMERCLHFLVEELKVVNALEAQNRIFFVSAKEVLSARKQKAQGMPESGVALAEGFHARLQEFQNFEQIFEECISQSAVKTKFEQHTIRAKQILATVKNIMDSVNLAAEDKRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEEEIARLPKEIDQLEKIQNNSKLLRNKAVQLENELENFTKQFLPSSNEES bak_human Bcl-2 homologous antagonist/killer Apoptosis regulator BAK Bcl-2-like protein 7 BAK1 BAK BCL2L7 CDN1 protein protein human Homo sapiens Human MASGQGPGPPRQECGEPALPSASEEQVAQDTEEVFRSYVFYRHQQEQEAEGVAAPADPEMVTLPLQPSSTMGQVGRQLAIIGDDINRRYDSEFQTMLQHLQPTAENAYEYFTKIATSLFESGINWGRVVALLGFGYRLALHVYQHGLTGFLGQVTRFVVDFMLHHCIARWIAQRGGWVAALNLGNGPILNVLVVLGVVLLGQFVVRRFFKS bax_human Apoptosis regulator BAX BAX BCL2L4 Bcl-2-like protein 4 protein protein human Homo sapiens Human MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG mfn1_mouse Mitofusin-1 Mfn1 Transmembrane GTPase MFN1 protein protein mouse Mus musculus Mouse MAETVSPLKHFVLAKKAITAIFGQLLEFVTEGSHFVEATYRNPELDRIASEDDLVEIQGYRNKLAVIGEVLSRRHMKVAFFGRTSSGKSSVINAMLWDKVLPSGIGHTTNCFLSVEGTDGDKAYLMTEGSDEKKSVKTVNQLAHALHMDKDLKAGCLVHVFWPKAKCALLRDDLVLVDSPGTDVTTELDIWIDKFCLDADVFVLVANSESTLMNTEKHFFHKVNERLSKPNIFILNNRWDASASEPEYMEDVRRQHMERCLHFLVEELKVVSPSEARNRIFFVSAKEVLNSRKHKAQGMPEGGGALAEGFQARLQEFQNFEQTFEECISQSAVKTKFEQHTIRAKQILDTVKNILDSVNVAAAEKRVYSMEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPTPSVLKVYKSELNKHIEDGMGRNLADRCTNEVNASILQSQQEIIENLKPLLPAGIQNKLHTLIPCKKFDLSYDLNCHKLCSDFQEDIVFRFSLGWSSLVHRFLGSTNAQRVLLGLSEPIFQVPRSLASTPTAPSNPAAPDNAAQEELMITLITGLASLTSRTSMGIIVVGGVIWKTVGWKLISVTLSMYGALYLYERLTWTTRAKERAFKQQFVNYATEKLQMIVSFTSANCSHQVQQEMATTFARLCQQVDVTQKHLEEEIARLSKEIDQLEKIQNNSKLLRNKAVQLESELENFSKQFLHPSSGES mfn2-bak1-1 3158402 N/A 3158412 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false mouse Mus musculus Mouse N/A 3158413 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association false false false bak1-mfn1-2 3158403 N/A 3158414 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3158415 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3158416 unspecified role unspecified role bait bait human Homo sapiens Human association association false false false mfn2-bak1-2 3158404 N/A 3158412 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3158417 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3158416 unspecified role unspecified role prey prey human Homo sapiens Human association association false false false bak1-mfn1-3 3158405 N/A 3158416 unspecified role unspecified role fluorescence donor fluorescence donor ECFP-tag ECFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3158418 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept EYFP-tag EYFP-tag eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction false false false bax-mfn1-1 3158406 N/A 3158417 unspecified role unspecified role fluorescence donor fluorescence donor ECFP-tag ECFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3158418 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept EYFP-tag EYFP-tag eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction false false false mfn1-bak1-1 3158407 N/A 3158418 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3158416 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3158417 unspecified role unspecified role prey prey human Homo sapiens Human association association false false false mfn1-bak1-2 3158408 N/A 3158413 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3158418 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false mouse Mus musculus Mouse physical association physical association false false false mfn2-bak1-3 3158409 N/A 3158412 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false mouse Mus musculus Mouse N/A 3158416 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association false false false bak1-mfn2-1 3158410 N/A 3158412 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept EYFP-tag EYFP-tag eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3158416 unspecified role unspecified role fluorescence donor fluorescence donor ECFP-tag ECFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction false false false bax-mfn2-1 3158411 N/A 3158412 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept EYFP-tag EYFP-tag eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3158417 unspecified role unspecified role fluorescence donor fluorescence donor ECFP-tag ECFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction false false false