DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ schwarz_romond-2007-1 ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. DIP original experiment: DIP-66831X Nature structural & molecular biology 2007 IMEx The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. Only protein-protein interactions imex curation schwarz_romond-2007-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. DIP original experiment: DIP-66832X Nature structural & molecular biology 2007 IMEx The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. Only protein-protein interactions imex curation schwarz_romond-2007-3 in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. DIP original experiment: DIP-66827X Nature structural & molecular biology 2007 IMEx The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. Only protein-protein interactions imex curation schwarz_romond-2007-4 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. DIP original experiment: DIP-66828X Nature structural & molecular biology 2007 IMEx The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. Only protein-protein interactions imex curation schwarz_romond-2007-5 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. DIP original experiment: DIP-66829X Nature structural & molecular biology 2007 IMEx The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. Only protein-protein interactions imex curation schwarz_romond-2007-6 ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. DIP original experiment: DIP-66830X Nature structural & molecular biology 2007 IMEx The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. Only protein-protein interactions imex curation dvl2_mouse Segment polarity protein dishevelled homolog DVL-2 Dvl2 DSH homolog 2 protein protein mouse Mus musculus Mouse MAGSSAGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDTPQPEVAPPAHESRTELVPPPPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRDRPRRRDSSEHGAGGHRPGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEDDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRMERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDIVHKPGPIVLTVAKCWDPSPQAYFTLPRNEPIQPIDPAAWVSHSAALTGAFPAYPGSSSMSTITSGSSLPDGCEGRGLSVHMDMASVTKAMAAPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFPERREARKYASGLLKAGLIRHTVNKITFSEQCYYVFGDLSGGCESYLVNLSLNDNDGSSGASDQDTLAPLPGATPWPLLPTFSYQYPAPHPYSPQPPPYHELSSYTYGGGSASSQHSEGSRSSGSTRSDGGAGRTGRPEERAPESKSGSGSESELSSRGGSLRRGGEPGGTGDGGPPPSRGSTGAPPNLRALPGLHPYGAPSGMALPYNPMMVVMMPPPPPPVSTAVQPPGAPPVRDLGSVPPELTASRQSFHMAMGNPSEFFVDVM axin1_rat Axin-1 Axin1 Axin Axis inhibition protein 1 protein protein rat Rattus norvegicus (Rat) Rat MNVQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDSRPVNHSFCSGKGTSIKSETSTATPRRSDLDLGYEPEGSASPTPPYLRWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACSGFRKLEPCDSNEEKRLKLARAIYRKYILDSNGIVSRQTKPATKSFIKDCVMKQQIDPAMFDQAQTEIQSTMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGMSGYLPTLNEDEEWKCDQDADEDDGRDSVPPSRLTQKLLLETAAPRAPSSRRYNEGRELRYGSWREPVNPYYVNSGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESVQVNGRVPLPHIPRTYRMPKEIRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGEMPSGPMASHKLPSVPAWHHFPPRYVDMGCSGLRDAHEENPESILDEHVQRVMRTPGCQSPGPGHRSPDSGHVAKTAVLGGTASGHGKHAPKLGLKLDSAGLHHHRHVHHHVHHNSARPKEQMEAEAARRVQSSFSWGPETHGHAKPRSYSESTGTNPSAGDLAFGGKASAPSKRNTKKAESGKNASAEVPSTTEDAEKNQKIMQWIIEGEKEISRHRKAGHGSSGMRKQQAHESSRPLSIERPGAVHPWVSAQLRNSVQPSHLFIQDPTMPPNPAPNPLTQLEEARRRLEEEEKRANKLPSKQRTKSQRKAGGGSAPPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAILPVFEEKIIGKVEKVD BB1EE9CCECF6D487 dvl2-1 3157003 N/A 3157009 unspecified role unspecified role neutral component neutral component 1-114 binding region 1-114 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false Q60838:p.Tyr27Asp Y27D mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q60838:p.Phe43Ser F43S mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q60838:p.[Val67Ala;Lys68Ala] V67A K68A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false raxn1-1 3157004 N/A 3157010 unspecified role unspecified role unspecified role unspecified role 713-832 binding region 713-832 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false dvl2-dvl2-7 3157005 N/A 3157009 unspecified role unspecified role prey prey 1-114 binding region 1-114 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false Q60838:p.Tyr27Asp Y27D mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q60838:p.Phe43Ser F43S mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q60838:p.[Val67Ala;Lys68Ala] V67A K68A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3157009 unspecified role unspecified role bait bait 1-114 binding region 1-114 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q60838:p.Tyr27Asp Y27D mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q60838:p.Phe43Ser F43S mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q60838:p.[Val67Ala;Lys68Ala] V67A K68A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false dvl2-dvl2-8 3157006 N/A 3157009 unspecified role unspecified role prey prey 1-114 binding region 1-114 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false Q60838:p.Phe43Ser F43S mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q60838:p.[Val67Ala;Lys68Ala] V67A K68A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false HA Tag HA Tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3157009 unspecified role unspecified role bait bait FLAG Tag FLAG Tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q60838:p.[Val67Ala;Lys68Ala] V67A K68A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q60838:p.Phe43Ser F43S mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false dvl2-2 3157007 N/A 3157009 unspecified role unspecified role neutral component neutral component 1-114 binding region 1-114 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false Q60838:p.Tyr27Asp Y27D mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q60838:p.Phe43Ser F43S mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q60838:p.[Val67Ala;Lys68Ala] V67A K68A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false dvl2-3 3157008 N/A 3157009 unspecified role unspecified role neutral component neutral component 1-114 binding region 1-114 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false Q60838:p.Tyr27Asp Y27D mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q60838:p.Phe43Ser F43S mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q60838:p.[Val67Ala;Lys68Ala] V67A K68A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false