DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ sato-2007-5 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Sato Y., Fukai S., Ishitani R., Nureki O. DIP original experiment: DIP-261412X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Crystal structure of the Sec4p.Sec2p complex in the nucleotide exchanging intermediate state. Sato Y., Fukai S., Ishitani R., Nureki O. Only protein-protein interactions imex curation sato-2007-6 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Sato Y., Fukai S., Ishitani R., Nureki O. DIP original experiment: DIP-261413X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Crystal structure of the Sec4p.Sec2p complex in the nucleotide exchanging intermediate state. Sato Y., Fukai S., Ishitani R., Nureki O. Only protein-protein interactions imex curation sec4_yeast Ras-related protein SEC4 SEC4 SRO6 Suppressor of RHO3 protein 6 YFL005W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGLRTVSASSGNGKSYDSIMKILLIGDSGVGKSCLLVRFVEDKFNPSFITTIGIDFKIKTVDINGKKVKLQLWDTAGQERFRTITTAYYRGAMGIILVYDVTDERTFTNIKQWFKTVNEHANDEAQLLLVGNKSDMETRVVTADQGEALAKELGIPFIESSAKNDDNVNEIFFTLAKLIQEKIDSNKLVGVGNGKEGNISINSGSGNSSKSNCC 7DEDF7DCC7533BA7 sec2_yeast Rab guanine nucleotide exchange factor SEC2 SEC2 YNL272C N0641 GDP-GTP exchange factor SEC2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDASEEAKRVSIQVTSLSTQLIESVDKQSHLEEQLNKSLKTIASQKAAIENYNQLKEDYNTLKRELSDRDDEVKRLREDIAKENELRTKAEEEADKLNKEVEDLTASLFDEANNMVADARKEKYAIEILNKRLTEQLREKDTLLDTLTLQLKNLKKVMHSLDNESTVTNNSNRYSTILSDSATSSSTSLNKVPTSYSLASQDIYSGIVYSPSISSIRYDISLYNEFLKFVAALPRCENIKATSTESKLIRRLVNDEIQPILKIDNASGIGWLVKKTLLSLIIDGLVVVEPLSGVNATYQIGYNSSSPAKQATSNMPKMFKFPLDSPPVAVHAACSFCGESRDDIIEHARMYILKTLHKTDDGKEQVTNTYPLCHWCLLKLRQTCEIFAFLRSLKVGAWHLEKLTTQNITKEDLEKFSEVTKHTKRDGRVSSQDKKTKRLSFMAGLGINSSTKNKPKMEIFSSETNAKPGQPTTNIQRAWLQLCKLRCILHWTHIGIWAVDDSISSKIGPLVEDDSDEDQNDAISVRLQDKALWKQDAKRPFSSSSAEESQKSDAFDFESGDMENEITGESSSDESSSDGSSTDNSTADSSSEDESSLADSTTSSADSSSPESIDNGEGDDTVTKDDKSSIKSANNNEENSDCGDKKGRSIIKKKAPQRKIQKKKLLQDLDDLEEQFREESAIDQTEFENAESNVKQNISSKRASSGDENSKKDNNEKTLKTNLTIGDKTQEQIGENSPSSGLHASSSNDDNFDDAQEQQ 7F5A9B2343CBE835 sec2p-sec4p-1 3156152 N/A 3156154 unspecified role unspecified role unspecified role unspecified role Region 19-187 Region 19-187 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3156155 unspecified role unspecified role unspecified role unspecified role Region 51-142 Region 51-142 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false sec2p-sec4p-2 3156153 N/A 3156154 unspecified role unspecified role unspecified role unspecified role Region 19-187 Region 19-187 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3156155 unspecified role unspecified role unspecified role unspecified role Region 51-142 Region 51-142 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false