DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ inoue-2007-5 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Inoue D., Ohe M., Kanemori Y., Nobui T., Sagata N. DIP original experiment: DIP-255226X Nature 2007 IMEx A direct link of the Mos-MAPK pathway to Erp1/Emi2 in meiotic arrest of Xenopus laevis eggs. Inoue D., Ohe M., Kanemori Y., Nobui T., Sagata N. Only protein-protein interactions imex curation inoue-2007-6 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Inoue D., Ohe M., Kanemori Y., Nobui T., Sagata N. DIP original experiment: DIP-255228X Nature 2007 IMEx A direct link of the Mos-MAPK pathway to Erp1/Emi2 in meiotic arrest of Xenopus laevis eggs. Inoue D., Ohe M., Kanemori Y., Nobui T., Sagata N. Only protein-protein interactions imex curation inoue-2007-7 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Inoue D., Ohe M., Kanemori Y., Nobui T., Sagata N. DIP original experiment: DIP-255227X Nature 2007 IMEx A direct link of the Mos-MAPK pathway to Erp1/Emi2 in meiotic arrest of Xenopus laevis eggs. Inoue D., Ohe M., Kanemori Y., Nobui T., Sagata N. Only protein-protein interactions imex curation fbx43_xenla F-box only protein 43 fbxo43 emi2 erp1 fbx26 Early mitotic inhibitor 2 Emi1-related protein 1 F-box protein 26 Xerp1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MANLLENFAAHHSMTAGAKKKADHQDTSVSQDSGYSDSLKGFSPDSHKSGNFLETVTEGYENSENIDPTLILSPIKYELSWGADTRESKQLAPLYETPRVGKKEFSLRRRLLISKATSGGNLDFDVSVCSAESCGREKSLRRIPSHEGSLSNSFADSPRDGSYEPIATSTLKTESESGTSCKKWRLSFAQQRSSTLDDSKSDSIPLPEVENISPVQHSLASSTDDSILYEETIFGAPTTPTCNFIVKEEFQTPISNLAANFRFNLCTPDVGHVSDFDISVTEDSAFHSLSLDKSQDSITDHEGSFQELIQKPRETSKAVNNKSRLRKLDRCRRLSTLRERGSQSEVEEEGNEVPVLSSAYKLKVARASVDEENEFSSDESRVHSLLSSDDLTGKPALRVLHEMLLRSTRKRPQQATVQDLLGSSGCFELPEDSLSRLIGRKMGLETFDILAELKNRNLKHILASILDLVNAASICSMCRVSRDWRDVVLQDKSAHQRRKAYIKKLKTEAEQGRQLSFEDSATRLNILSRSALRSVQIQARSAFRTPTSSLTPGDNKSIHSASKHQEYLKVAKTLFTDEALKPCPRCQYPAKYQALKKRGTCSRKDCGFDFCSLCLCTFHGSKECGTGSAKRIPKKEALPGSAQSKRNLKRL mint q9ptn1_xenla Ribosomal protein S6 kinase RSK2 rps6ka3 XELAEV_18011847mg rps6ka3.L protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MPLAQLADPWQKVPVQKVESENGQQFADESMCEEDNENNDEGNINEIAITHHVKEGHEKADPSQFELLKVLGQGSFGKVFLVRKISGTDAGQLYAMKVLKKATLKVRDRVRTKMERDILVEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKYYLAELALALDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKESIDHEKKAYSFCGTVEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGTLPFQGKDRKETMTMILKAKLGMPQFLTPEAQSLLRMLFKRNPTNRLGAGPDGVEEIKRHPFFVTIDWNKLFRREIQPPFKPATGGPEDTFYFDPEFTAKTPKDSPGIPASANAHQLFRGFSFVAISSEDENQAMQTVGVHAIVPLHRNSIQFTDGYELKEDIGVGSYSICKRCIHKGTNMEYAVKIIDKSKRDPTEEIEILLRYGQHPNIITLKDVYDDGKYVYLVTELMKGGELLDKILRQKFFSEREASAVLHTITKTAEYLHSQWVVHRDLKPSNILYVDESGNPESIRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDAACDIWSLGVLLYTMLTGYTPFANGPEDTPEEILARIGSGKFSLSGGYWNSVSDIAKDLVSKMLHVDPHQRLTAAQVLKHPWIVHCDQLPQFQLNRQDAPHLVKGAMAATYSALNLNPMSPVLAPVGRSTLAQRRGVKKITSTAL fbxo43-rps6ka3-2 3154843 N/A 3154846 enzyme target enzyme target substrate substrate unspecified role unspecified role pSer pSer opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain false pThr pThr opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain false Q8AXF4:p.[Ser335Ala;Thr336Ala] S335A/T336A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3154847 enzyme enzyme unspecified role unspecified role in vitro In vitro phosphorylation phosphorylation reaction phosphorylation false false false fbxo43-rps6ka3-3 3154844 N/A 3154846 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 200-400 Region 200-400 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q8AXF4:p.[Ser335Ala;Thr336Ala] S335A/T336A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q8AXF4:p.[Thr315_Ser316delinsAlaAla;Ser335_Thr336delinsAlaAla;Ser342Ala] S315A/S316A/S335A/T336A/S342A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3154847 enzyme enzyme unspecified role unspecified role in vitro In vitro phosphorylation phosphorylation reaction phosphorylation false false false fbxo43-rps6ka3-4 3154845 N/A 3154846 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 200-400 Region 200-400 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q8AXF4:p.[Ser335Ala;Thr336Ala] S335A/T336A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false pSer pSer opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain false pThr pThr opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3154847 enzyme enzyme unspecified role unspecified role in vitro In vitro phosphorylation phosphorylation reaction phosphorylation false false false