DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ eakin-2007-1 in vitro In vitro ms of complexes mass spectrometry studies of complexes ms of complexes predetermined predetermined participant predetermined Eakin CM., Maccoss MJ., Finney GL., Klevit RE. DIP original experiment: DIP-261290X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Estrogen receptor alpha is a putative substrate for the BRCA1 ubiquitin ligase. Eakin CM., Maccoss MJ., Finney GL., Klevit RE. Only protein-protein interactions imex curation eakin-2007-2 in vitro In vitro comigration in sds comigration in sds page comigration in sds anti tag western anti tag western blot anti tag western Eakin CM., Maccoss MJ., Finney GL., Klevit RE. DIP original experiment: DIP-261291X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Estrogen receptor alpha is a putative substrate for the BRCA1 ubiquitin ligase. Eakin CM., Maccoss MJ., Finney GL., Klevit RE. Only protein-protein interactions imex curation ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG p03372-1 Estrogen receptor ESR1 ESR NR3A1 ER-alpha Estradiol receptor Nuclear receptor subfamily 3 group A member 1 ER66 Long hER-alpha66 protein protein human Homo sapiens Human MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV esr1-ub-1 3154575 N/A 3154577 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3154578 unspecified role unspecified role unspecified role unspecified role K302 K302 ubiquitinylated lysine ubiquitinylated lysine modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false Region 302-552 Region 302-552 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli covalent binding covalent binding false false false esr1-ub-2 3154576 N/A 3154577 unspecified role unspecified role unspecified role unspecified role T7-tag T7-tag t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false ecolx Escherichia coli N/A 3154578 unspecified role unspecified role unspecified role unspecified role Region 302-552 Region 302-552 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false