DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ shakhnovich-2007-1 ecolx Escherichia coli 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Shakhnovich EA., Hung DT., Pierson E., Lee K., Mekalanos JJ. DIP original experiment: DIP-261038X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Virstatin inhibits dimerization of the transcriptional activator ToxT. Shakhnovich EA., Hung DT., Pierson E., Lee K., Mekalanos JJ. Only protein-protein interactions imex curation shakhnovich-2007-2 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Shakhnovich EA., Hung DT., Pierson E., Lee K., Mekalanos JJ. DIP original experiment: DIP-261039X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Virstatin inhibits dimerization of the transcriptional activator ToxT. Shakhnovich EA., Hung DT., Pierson E., Lee K., Mekalanos JJ. Only protein-protein interactions imex curation tcpn_vibch TCP pilus virulence regulatory protein tcpN toxT VC_0838 protein protein vibch Vibrio cholerae MIGKKSFQTNVYRMSKFDTYIFNNLYINDYKMFWIDSGIAKLIDKNCLVSYEINSSSIILLKKNSIQRFSLTSLSDENINVSVITISDSFIRSLKSYILGDLMIRNLYSENKDLLLWNCEHNDIAVLSEVVNGFREINYSDEFLKVFFSGFFSKVEKKYNSIFITDDLDAMEKISCLVKSDITRNWRWADICGELRTNRMILKKELESRGVKFRELINSIRISYSISLMKTGEFKIKQIAYQSGFASVSYFSTVFKSTMNVAPSEYLFMLTGVAEK tcpn-tcpn-1 3152584 N/A 3152586 unspecified role unspecified role prey prey Region 1-167 Region 1-167 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3152586 unspecified role unspecified role bait bait Region 1-167 Region 1-167 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false tcpn-1 3152585 N/A 3152586 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false