DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ strohmeier-2006-1 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Strohmeier M., Raschle T., Mazurkiewicz J., Rippe K., Sinning I., Fitzpatrick TB., Tews I. DIP original experiment: DIP-264282X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structure of a bacterial pyridoxal 5'-phosphate synthase complex. Strohmeier M., Raschle T., Mazurkiewicz J., Rippe K., Sinning I., Fitzpatrick TB., Tews I. Only protein-protein interactions imex curation strohmeier-2006-2 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Strohmeier M., Raschle T., Mazurkiewicz J., Rippe K., Sinning I., Fitzpatrick TB., Tews I. DIP original experiment: DIP-264279X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structure of a bacterial pyridoxal 5'-phosphate synthase complex. Strohmeier M., Raschle T., Mazurkiewicz J., Rippe K., Sinning I., Fitzpatrick TB., Tews I. Only protein-protein interactions imex curation strohmeier-2006-3 ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Strohmeier M., Raschle T., Mazurkiewicz J., Rippe K., Sinning I., Fitzpatrick TB., Tews I. DIP original experiment: DIP-264283X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structure of a bacterial pyridoxal 5'-phosphate synthase complex. Strohmeier M., Raschle T., Mazurkiewicz J., Rippe K., Sinning I., Fitzpatrick TB., Tews I. Only protein-protein interactions imex curation strohmeier-2006-4 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Strohmeier M., Raschle T., Mazurkiewicz J., Rippe K., Sinning I., Fitzpatrick TB., Tews I. DIP original experiment: DIP-264286X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structure of a bacterial pyridoxal 5'-phosphate synthase complex. Strohmeier M., Raschle T., Mazurkiewicz J., Rippe K., Sinning I., Fitzpatrick TB., Tews I. Only protein-protein interactions imex curation strohmeier-2006-5 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Strohmeier M., Raschle T., Mazurkiewicz J., Rippe K., Sinning I., Fitzpatrick TB., Tews I. DIP original experiment: DIP-264280X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structure of a bacterial pyridoxal 5'-phosphate synthase complex. Strohmeier M., Raschle T., Mazurkiewicz J., Rippe K., Sinning I., Fitzpatrick TB., Tews I. Only protein-protein interactions imex curation strohmeier-2006-6 ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Strohmeier M., Raschle T., Mazurkiewicz J., Rippe K., Sinning I., Fitzpatrick TB., Tews I. DIP original experiment: DIP-264284X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structure of a bacterial pyridoxal 5'-phosphate synthase complex. Strohmeier M., Raschle T., Mazurkiewicz J., Rippe K., Sinning I., Fitzpatrick TB., Tews I. Only protein-protein interactions imex curation strohmeier-2006-7 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Strohmeier M., Raschle T., Mazurkiewicz J., Rippe K., Sinning I., Fitzpatrick TB., Tews I. DIP original experiment: DIP-264281X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structure of a bacterial pyridoxal 5'-phosphate synthase complex. Strohmeier M., Raschle T., Mazurkiewicz J., Rippe K., Sinning I., Fitzpatrick TB., Tews I. Only protein-protein interactions imex curation strohmeier-2006-8 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Strohmeier M., Raschle T., Mazurkiewicz J., Rippe K., Sinning I., Fitzpatrick TB., Tews I. DIP original experiment: DIP-264285X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structure of a bacterial pyridoxal 5'-phosphate synthase complex. Strohmeier M., Raschle T., Mazurkiewicz J., Rippe K., Sinning I., Fitzpatrick TB., Tews I. Only protein-protein interactions imex curation pdxt_bacsu Pyridoxal 5'-phosphate synthase subunit PdxT Pdx2 Pyridoxal 5'-phosphate synthase glutaminase subunit pdxT yaaE BSU00120 protein protein bacsu Bacillus subtilis MLTIGVLGLQGAVREHIHAIEACGAAGLVVKRPEQLNEVDGLILPGGESTTMRRLIDTYQFMEPLREFAAQGKPMFGTCAGLIILAKEIAGSDNPHLGLLNVVVERNSFGRQVDSFEADLTIKGLDEPFTGVFIRAPHILEAGENVEVLSEHNGRIVAAKQGQFLGCSFHPELTEDHRVTQLFVEMVEEYKQKALV mint pdxs_bacsu Pyridoxal 5'-phosphate synthase subunit PdxS Pdx1 pdxS Superoxide-inducible protein 7 yaaD BSU00110 protein protein bacsu Bacillus subtilis MAQTGTERVKRGMAEMQKGGVIMDVINAEQAKIAEEAGAVAVMALERVPADIRAAGGVARMADPTIVEEVMNAVSIPVMAKARIGHIVEARVLEAMGVDYIDESEVLTPADEEFHLNKNEYTVPFVCGCRDLGEATRRIAEGASMLRTKGEPGTGNIVEAVRHMRKVNAQVRKVVAMSEDELMTEAKNLGAPYELLLQIKKDGKLPVVNFAAGGVATPADAALMMQLGADGVFVGSGIFKSDNPAKFAKAIVEATTHFTDYKLIAELSKELGTAMKGIEISNLLPEQRMQERGW mint pdxt-1 3151155 N/A 3151163 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false pdxs-1 3151156 N/A 3151164 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false pdxs-2 3151157 N/A 3151164 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false pdxs-pdxt-1 3151158 N/A 3151163 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3151164 unspecified role unspecified role unspecified role unspecified role Region 1-18 Region 1-18 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pdxs-pdxt-2 3151159 N/A 3151163 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3151164 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false pdxs-pdxt-3 3151160 N/A 3151163 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3151164 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false pdxs-pdxt-4 3151161 N/A 3151163 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3151164 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false pdxs-pdxt-5 3151162 N/A 3151163 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3151164 unspecified role unspecified role unspecified role unspecified role Region1-18 Region1-18 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false