DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ zamborlini-2006-1 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zamborlini A., Usami Y., Radoshitzky SR., Popova E., Palu G., Göttlinger H. DIP original experiment: DIP-264249X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Release of autoinhibition converts ESCRT-III components into potent inhibitors of HIV-1 budding. Zamborlini A., Usami Y., Radoshitzky SR., Popova E., Palu G., Göttlinger H. Only protein-protein interactions imex curation zamborlini-2006-2 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zamborlini A., Usami Y., Radoshitzky SR., Popova E., Palu G., Göttlinger H. DIP original experiment: DIP-264254X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Release of autoinhibition converts ESCRT-III components into potent inhibitors of HIV-1 budding. Zamborlini A., Usami Y., Radoshitzky SR., Popova E., Palu G., Göttlinger H. Only protein-protein interactions imex curation zamborlini-2006-3 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zamborlini A., Usami Y., Radoshitzky SR., Popova E., Palu G., Göttlinger H. DIP original experiment: DIP-264251X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Release of autoinhibition converts ESCRT-III components into potent inhibitors of HIV-1 budding. Zamborlini A., Usami Y., Radoshitzky SR., Popova E., Palu G., Göttlinger H. Only protein-protein interactions imex curation zamborlini-2006-4 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zamborlini A., Usami Y., Radoshitzky SR., Popova E., Palu G., Göttlinger H. DIP original experiment: DIP-264253X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Release of autoinhibition converts ESCRT-III components into potent inhibitors of HIV-1 budding. Zamborlini A., Usami Y., Radoshitzky SR., Popova E., Palu G., Göttlinger H. Only protein-protein interactions imex curation zamborlini-2006-5 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zamborlini A., Usami Y., Radoshitzky SR., Popova E., Palu G., Göttlinger H. DIP original experiment: DIP-264250X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Release of autoinhibition converts ESCRT-III components into potent inhibitors of HIV-1 budding. Zamborlini A., Usami Y., Radoshitzky SR., Popova E., Palu G., Göttlinger H. Only protein-protein interactions imex curation zamborlini-2006-6 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zamborlini A., Usami Y., Radoshitzky SR., Popova E., Palu G., Göttlinger H. DIP original experiment: DIP-264252X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Release of autoinhibition converts ESCRT-III components into potent inhibitors of HIV-1 budding. Zamborlini A., Usami Y., Radoshitzky SR., Popova E., Palu G., Göttlinger H. Only protein-protein interactions imex curation q9y3e7-1 Charged multivesicular body protein 3 CHMP3 CGI149 NEDF VPS24 Chromatin-modifying protein 3 Neuroendocrine differentiation factor Vacuolar protein sorting-associated protein 24 CGI-149 Vps24alpha protein protein human Homo sapiens Human MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSKVTDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLRS chm4b_mouse Charged multivesicular body protein 4b Chmp4b Chromatin-modifying protein 4b protein protein mouse Mus musculus Mouse MSVFGKLFGAGGGKAGKGGPTPQEAIQRLRDTEEMLSKKQEFLEKKIEQELTAAKKHGTKNKRAALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGYAAKAMKAAHDNMDIDKVDELMQDIADQQELAEEISTAISKPVGFGEEFDEDELMAELEELEQEELDKNLLEISGPETVPLPNVPSVALPSKPAKKKEEEDDDMKELENWAGSM mint Component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In the ESCRT-III complex, it probably serves as an acceptor for ESCRT-I complex on endosomal membranes vps4a_human Vacuolar protein sorting-associated protein 4A VPS4-1 Protein SKD2 VPS4A VPS4 protein protein human Homo sapiens Human MTTSTLQKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCVQYLDRAEKLKDYLRSKEKHGKKPVKENQSEGKGSDSDSEGDNPEKKKLQEQLMGAVVMEKPNIRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGTLVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGSTPHNLTDANIHELARKTEGYSGADISIIVRDSLMQPVRKVQSATHFKKVCGPSRTNPSMMIDDLLTPCSPGDPGAMEMTWMDVPGDKLLEPVVCMSDMLRSLATTRPTVNADDLLKVKKFSEDFGQES stabp_human STAM-binding protein AMSH STAMBP Associated molecule with the SH3 domain of STAM Endosome-associated ubiquitin isopeptidase protein protein human Homo sapiens Human MSDHGDVSLPPEDRVRALSQLGSAVEVNEDIPPRRYFRSGVEIIRMASIYSEEGNIEHAFILYNKYITLFIEKLPKHRDYKSAVIPEKKDTVKKLKEIAFPKAEELKAELLKRYTKEYTEYNEEKKKEAEELARNMAIQQELEKEKQRVAQQKQQQLEQEQFHAFEEMIRNQELEKERLKIVQEFGKVDPGLGGPLVPDLEKPSLDVFPTLTVSSIQPSDCHTTVRPAKPPVVDRSLKPGALSNSESIPTIDGLRHVVVPGRLCPQFLQLASANTARGVETCGILCGKLMRNEFTITHVLIPKQSAGSDYCNTENEEELFLIQDQQGLITLGWIHTHPTQTAFLSSVDLHTHCSYQMMLPESVAIVCSPKFQETGFFKLTDHGLEEISSCRQKGFHPHSKDPPLFCSCSHVTVVDRAVTITDLR chmp3-chmp3-3 3150921 N/A 3150927 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Region 1-150 Region 1-150 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3150927 unspecified role unspecified role bait bait Region 151-179 Region 151-179 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false chmp3-chmp3-4 3150922 N/A 3150927 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Region 1-113 Region 1-113 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3150927 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 151-222 Region 151-222 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false chmp4b-chmp4b-7 3150923 N/A 3150928 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Region 1-153 Region 1-153 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3150928 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 154-224 Region 154-224 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false chmp3-vps4a-1 3150924 N/A 3150929 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3150927 unspecified role unspecified role bait bait Region 151-222 Region 151-222 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false chmp3-chmp3-5 3150925 N/A 3150927 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3150927 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 151-222 Region 151-222 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false chmp3-stambp-12 3150926 N/A 3150930 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3150927 unspecified role unspecified role bait bait Region 151-222 Region 151-222 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q9Y3E7-1:p.[Arg216Ala;Leu217Ala] R216A/L217A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false