DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ margarit-2006-1 in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Margarit SM., Davidson W., Frego L., Stebbins CE. DIP original experiment: DIP-66357X Structure (London, England : 1993) 2006 IMEx A steric antagonism of actin polymerization by a salmonella virulence protein. Margarit SM., Davidson W., Frego L., Stebbins CE. Only protein-protein interactions imex curation margarit-2006-2 in vitro In vitro fluorescence fluorescence technology fluorescence predetermined predetermined participant predetermined Margarit SM., Davidson W., Frego L., Stebbins CE. DIP original experiment: DIP-66356X Structure (London, England : 1993) 2006 IMEx A steric antagonism of actin polymerization by a salmonella virulence protein. Margarit SM., Davidson W., Frego L., Stebbins CE. Only protein-protein interactions imex curation acts_rabit Actin, alpha skeletal muscle ACTA1 ACTA Alpha-actin-1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF spvb_saltm Mono(ADP-ribosyl)transferase SpvB spvB 65 kDa virulence protein NAD(+)--arginine ADP-ribosyltransferase Toxin SpvB mkaA protein protein saltm Salmonella typhimurium MLILNGFSSATLALITPPFLPKGGKALSQSGPDGLASITLPLPISAERGFAPALALPYSSGGGNGPFGVGWSCATMSIARRTSHGVPQYNDSDEFLGPDGEVLVQTLSTGDAPNPVTCFAYGDVSFPQSYTVTRYQPRTESSFYRLEYWVGNSNGDDFWLLHDSNGILHLLGKTAAARLSDPQAASHTAQWLVEESVTPAGEHIYYSYLAENGDNVDLNGNEAGRDRSAMRYLSKVQYGNATPAADLYLWTSATPAVQWLFTLVFDYGERGVDPQVPPAFTAQNSWLARQDPFSLYNYGFEIRLLRLCRQVLMFHHFPDELGEADTLVSRLLLEYDENPIRTQLCAARTLAYEGDGYRRAPVNNMMPPPPPPPMMGGNSSRPKSKWAIVEESKQIQALRYYSAQGYSVINKYLRGDDYPETQAKETLLSRDYLSTNEPSDEEFKNAMSVYINDIAEGLSSLPETDHRVVYRGLKLDKPALSDVLKEYTTIVNIIIDKAFMSTSPDKAWINDTILNIYLEKGHKGRILGDVAHFKGEAEMLFPPNTKLKIESIVNCGSQDFASQLSKLRLSDDATADTNRIKRIINMRVLNS 2B933EAA4C8075E3 acta1-spvb-1 3146781 N/A 3146783 enzyme target enzyme target substrate substrate unspecified role unspecified role ADP-Ribosylated ADP-Ribosylated adprib1res monoadenosine diphosphoribosyl (ADP-ribosyl) modified residue ADP-rybosylation (from NAD) ADPRib1Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false rabit Oryctolagus cuniculus (Rabbit) Rabbit N/A 3146784 enzyme enzyme unspecified role unspecified role Region 390-591 Region 390-591 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false acta1-spvb-2 3146782 N/A 3146783 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit N/A 3146784 unspecified role unspecified role unspecified role unspecified role Region 390-591 Region 390-591 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false