DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ gracheva-2006-1 in vitro In vitro affinity techniques affinity technology affinity techniques predetermined predetermined participant predetermined Gracheva EO., Burdina AO., Holgado AM., Berthelot-Grosjean M., Ackley BD., Hadwiger G., Nonet ML., Weimer RM., Richmond JE. DIP original experiment: DIP-66482X PLoS biology 2006 IMEx Tomosyn inhibits synaptic vesicle priming in Caenorhabditis elegans. Gracheva EO., Burdina AO., Holgado AM., Berthelot-Grosjean M., Ackley BD., Hadwiger G., Nonet ML., Weimer RM., Richmond JE. Only protein-protein interactions imex curation gracheva-2006-2 in vitro In vitro affinity techniques affinity technology affinity techniques anti tag western anti tag western blot anti tag western Gracheva EO., Burdina AO., Holgado AM., Berthelot-Grosjean M., Ackley BD., Hadwiger G., Nonet ML., Weimer RM., Richmond JE. DIP original experiment: DIP-66484X PLoS biology 2006 IMEx Tomosyn inhibits synaptic vesicle priming in Caenorhabditis elegans. Gracheva EO., Burdina AO., Holgado AM., Berthelot-Grosjean M., Ackley BD., Hadwiger G., Nonet ML., Weimer RM., Richmond JE. Only protein-protein interactions imex curation gracheva-2006-3 in vitro In vitro affinity techniques affinity technology affinity techniques predetermined predetermined participant predetermined Gracheva EO., Burdina AO., Holgado AM., Berthelot-Grosjean M., Ackley BD., Hadwiger G., Nonet ML., Weimer RM., Richmond JE. DIP original experiment: DIP-66481X PLoS biology 2006 IMEx Tomosyn inhibits synaptic vesicle priming in Caenorhabditis elegans. Gracheva EO., Burdina AO., Holgado AM., Berthelot-Grosjean M., Ackley BD., Hadwiger G., Nonet ML., Weimer RM., Richmond JE. Only protein-protein interactions imex curation gracheva-2006-4 in vitro In vitro affinity techniques affinity technology affinity techniques predetermined predetermined participant predetermined Gracheva EO., Burdina AO., Holgado AM., Berthelot-Grosjean M., Ackley BD., Hadwiger G., Nonet ML., Weimer RM., Richmond JE. DIP original experiment: DIP-66483X PLoS biology 2006 IMEx Tomosyn inhibits synaptic vesicle priming in Caenorhabditis elegans. Gracheva EO., Burdina AO., Holgado AM., Berthelot-Grosjean M., Ackley BD., Hadwiger G., Nonet ML., Weimer RM., Richmond JE. Only protein-protein interactions imex curation syb1_caeel Synaptobrevin-1 snb-1 Synaptobrevin-related protein 1 T10H9.4 protein protein caeel Caenorhabditis elegans MDAQGDAGAQGGSQGGPRPSNKRLQQTQAQVDEVVGIMKVNVEKVLERDQKLSQLDDRADALQEGASQFEKSAATLKRKYWWKNIKMMIIMCAIVVILIIIIVLWAGGK stx1a_caeel Syntaxin-1A homolog unc-64 Uncoordinated protein 64 F56A8.7 protein protein caeel Caenorhabditis elegans MTKDRLSALKAAQSEDEQDDDMHMDTGNAQYMEEFFEQVEEIRGSVDIIANNVEEVKKKHSAILSNPVNDQKTKEELDELMAVIKRAANKVRGKLKLIENAIDHDEQGAGNADLRIRKTQHSTLSRRFVEVMTDYNKTQTDYRERCKGRIQRQLDIAGKQVGDEDLEEMIESGNPGVFTQGIITDTQQAKQTLADIEARHNDIMKLESSIRELHDMFMDMAMLVESQGEMVDRIEYNVEHAKEFVDRAVADTKKAVQYQSKARRKKICILVTGVILITGLIIFILFYAKVL snp25_rat Synaptosomal-associated protein 25 Snap25 Snap Synaptosomal-associated 25 kDa protein Super protein protein protein rat Rattus norvegicus (Rat) Rat MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG tom1ct Tomosyn homolog protein 1, isoform c protein protein caeel Caenorhabditis elegans This sequence has been withdrawn from Uniprot. The sequence has been withdrawn from uniprot. o62414_caeel Synaptosomal-associated protein ric-4 CELE_Y22F5A.3 Y22F5A.3 protein protein caeel Caenorhabditis elegans MSGDDDIPEGLEAINLKMNATTDDSLESTRRMLALCEESKEAGIKTLVMLDDQGEQLERCEGALDTINQDMKEAEDHLKGMEKCCGLCVLPWNKTDDFEKTEFAKAWKKDDDGGVISDQPRITVGDSSMGPQGGYITKITNDAREDEMDENVQQVSTMVGNLRNMAIDMSTEVSNQNRQLDRIHDKAQSNEVRVESANKRAKNLITK unc_64-snb_1-1 3146693 N/A 3146697 unspecified role unspecified role prey prey His6t7 tag His6t7 tag t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag full dna sequence full identification by DNA sequencing full dna sequence n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3146698 unspecified role unspecified role bait bait GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag full dna sequence full identification by DNA sequencing full dna sequence n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Binding region 1- 266 Binding region 1- 266 sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false unc_64-snap25-1 3146694 N/A 3146699 unspecified role unspecified role prey prey His6 tag His6 tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag full dna sequence full identification by DNA sequencing full dna sequence c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3146698 unspecified role unspecified role bait bait GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag full dna sequence full identification by DNA sequencing full dna sequence n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Binding region 1- 266 Binding region 1- 266 sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false unc_64-tom1ct-1 3146695 N/A 3146700 unspecified role unspecified role prey prey His6T7 His6T7 t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag full dna sequence full identification by DNA sequencing full dna sequence n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false Binding region 1137-1211 Binding region 1137-1211 sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3146698 unspecified role unspecified role bait bait GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag full dna sequence full identification by DNA sequencing full dna sequence n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Binding region 1- 266 Binding region 1- 266 sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false unc_64-ric_4-1 3146696 N/A 3146701 unspecified role unspecified role prey prey His6 tag His6 tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag full dna sequence full identification by DNA sequencing full dna sequence c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3146698 unspecified role unspecified role bait bait GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag full dna sequence full identification by DNA sequencing full dna sequence n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Binding region 1- 266 Binding region 1- 266 sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false