DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ yunus-2006-1 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Yunus AA., Lima CD. DIP original experiment: DIP-66235X Nature structural & molecular biology 2006 IMEx Lysine activation and functional analysis of E2-mediated conjugation in the SUMO pathway. Yunus AA., Lima CD. Only protein-protein interactions imex curation yunus-2006-2 in vitro In vitro comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Yunus AA., Lima CD. DIP original experiment: DIP-66238X Nature structural & molecular biology 2006 IMEx Lysine activation and functional analysis of E2-mediated conjugation in the SUMO pathway. Yunus AA., Lima CD. Only protein-protein interactions imex curation yunus-2006-3 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Yunus AA., Lima CD. DIP original experiment: DIP-66239X Nature structural & molecular biology 2006 IMEx Lysine activation and functional analysis of E2-mediated conjugation in the SUMO pathway. Yunus AA., Lima CD. Only protein-protein interactions imex curation yunus-2006-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yunus AA., Lima CD. DIP original experiment: DIP-66236X Nature structural & molecular biology 2006 IMEx Lysine activation and functional analysis of E2-mediated conjugation in the SUMO pathway. Yunus AA., Lima CD. Only protein-protein interactions imex curation yunus-2006-5 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Yunus AA., Lima CD. DIP original experiment: DIP-66237X Nature structural & molecular biology 2006 IMEx Lysine activation and functional analysis of E2-mediated conjugation in the SUMO pathway. Yunus AA., Lima CD. Only protein-protein interactions imex curation sumo1_human Small ubiquitin-related modifier 1 SUMO1 Sentrin Ubiquitin-like protein SMT3C Ubiquitin-homology domain protein PIC1 Ubiquitin-like protein UBL1 GAP-modifying protein 1 SMT3 homolog 3 SMT3C SMT3H3 UBL1 OK/SW-cl.43 protein protein human Homo sapiens Human MSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGHSTV ubc9_human SUMO-conjugating enzyme UBC9 UBE2I p18 SUMO-protein ligase UBC9 UBCE9 Ubiquitin carrier protein 9 Ubiquitin carrier protein I Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I RING-type E3 SUMO transferase UBC9 protein protein human Homo sapiens Human MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS ragp1_human Ran GTPase-activating protein 1 RANGAP1 KIAA1835 SD protein protein human Homo sapiens Human MASEDIAKLAETLAKTQVAGGQLSFKGKSLKLNTAEDAKDVIKEIEDFDSLEALRLEGNTVGVEAARVIAKALEKKSELKRCHWSDMFTGRLRTEIPPALISLGEGLITAGAQLVELDLSDNAFGPDGVQGFEALLKSSACFTLQELKLNNCGMGIGGGKILAAALTECHRKSSAQGKPLALKVFVAGRNRLENDGATALAEAFRVIGTLEEVHMPQNGINHPGITALAQAFAVNPLLRVINLNDNTFTEKGAVAMAETLKTLRQVEVINFGDCLVRSKGAVAIADAIRGGLPKLKELNLSFCEIKRDAALAVAEAMADKAELEKLDLNGNTLGEEGCEQLQEVLEGFNMAKVLASLSDDEDEEEEEEGEEEEEEAEEEEEEDEEEEEEEEEEEEEEPQQRGQGEKSATPSRKILDPNTGEPAPVLSSPPPADVSTFLAFPSPEKLLRLGPKSSVLIAQQTDTSDPEKVVSAFLKVSSVFKDEATVRMAVQDAVDALMQKAFNSSSFNSNTFLTRLLVHMGLLKSEDKVKAIANLYGPLMALNHMVQQDYFPKALAPLLLAFVTKPNSALESCSFARHSLLQTLYKV p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD sumo1-ube2i-6 3144381 N/A 3144386 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli N/A 3144387 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli covalent binding covalent binding false false false rangap1-sumo1-1 3144382 N/A 3144386 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli N/A 3144388 unspecified role unspecified role neutral component neutral component 419-587 binding region 419-587 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli covalent binding covalent binding false false false sumo1-tp53-1 3144383 N/A 3144389 unspecified role unspecified role neutral component neutral component 320-393 binding region 320-393 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3144386 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli covalent binding covalent binding false false false rangap1-ube2i-3 3144384 N/A 3144388 unspecified role unspecified role neutral component neutral component binding region (419-587) binding region (419-587) sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3144387 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction false false false rangap1-ube2i-4 3144385 N/A 3144388 unspecified role unspecified role neutral component neutral component binding region (419-587) binding region (419-587) sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3144387 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction false false false