DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ sivaram-2006-1 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Sivaram MV., Furgason ML., Brewer DN., Munson M. DIP original experiment: DIP-66259X Nature structural & molecular biology 2006 IMEx The structure of the exocyst subunit Sec6p defines a conserved architecture with diverse roles. Sivaram MV., Furgason ML., Brewer DN., Munson M. Only protein-protein interactions imex curation sivaram-2006-2 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Sivaram MV., Furgason ML., Brewer DN., Munson M. DIP original experiment: DIP-66261X Nature structural & molecular biology 2006 IMEx The structure of the exocyst subunit Sec6p defines a conserved architecture with diverse roles. Sivaram MV., Furgason ML., Brewer DN., Munson M. Only protein-protein interactions imex curation sivaram-2006-3 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Sivaram MV., Furgason ML., Brewer DN., Munson M. DIP original experiment: DIP-66260X Nature structural & molecular biology 2006 IMEx The structure of the exocyst subunit Sec6p defines a conserved architecture with diverse roles. Sivaram MV., Furgason ML., Brewer DN., Munson M. Only protein-protein interactions imex curation sec6_yeast Exocyst complex component SEC6 YIL068C SEC6 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSDPLQQVCDLIKGDLSLERVRDIKEQLLKEKSVVEYQLNKESDKYYGEVEESLKLLNLSKNSVTSIKQQINEVNKLGNDNRFAINRYDILFRATKLYETVNTTSSIYDRIYNFVALMEHIERLLVAELAEDALETGCPHLLEIHFLLTSARDFQEQVVVMAKEATEDAQRTVMKLFSRLSGIISKFDKLLDGLTYDIVEMARAEQISLAIRLFKIYDLEEREDLRIEAIRNIIKKKEIEIEKSSIKKLPNSKNTARLQDETPKVIEYPTNKGLYQEIMSGTISTRTAPRGYKHFLINGINNSISEMFGEMREKYVGDQKFDVLDNMDWIFNELIIVKEHIANCCPPHWNIFEVYFDQYYKELHSLITDLVESEPETIIILDILAFDKTFQDTLKQDFGFTKSEVKSVIGDKEKETLFKDYLNLIVVKMTEWIGNLEKAEFDVFLERSTPPHSDSDGLLFLDGTKTCFQMFTQQVEVAAGTNQAKILVGVVERFSDLLTKRQKNWISKISEEIKKQINYNHKYDIDPESITPEDECPGGLVEYLIAVSNDQMKAADYAVAISSKYGKLVSKVYEKQITNHLEGTLDGFAEVAQCSSLGLITLMFDDLRKPYQEIFSKTWYMGSQAQQIADTLDEYLLDIKPQMNSVLFVNFIDNVIGETIIKFLTALSFEHSFKNKNNKFLEAMKRDFEIFYQLFVKVLDGNESKDTLITQNFTVMEFFMDLSCEPIDSILDIWQKYLEVYWDSRIDLLVGILKCRKDVSSSERKKIVQQATEMLHEYRRNMEANGVDREPTLMRRFVLEFEKQ 2B09BBBE15389239 sec8_yeast Exocyst complex component SEC8 SEC8 YP9499.11 YPR055W protein protein yeast Saccharomyces cerevisiae Baker's yeast MDYLKPAQKGRRRGLSINSLSETQQSAMNSSLDHLQNDLNRINLQWNRILSDNTNPLELALAFLDDTSVGLGHRYEEFNQLKSQIGSHLQDVVNEHSQVFNTNVASYGKAVSSIMQAQEQTLNLKNCLKEANEKITTDKGSLQELNDNNLKYTKMIDVLVNIEELLQIPEKIEENIRKENFHQVQILLERGFILMNNKSLKTVEILKPINQQLELQEHLLFNNLIEEIHDIMYSKSNKTNFTRVTNNDIFKIISISHNGFTSLENYLYNIVNIDIMEHSKTINKNLEQFIHDQSLNKGNIMLQENAATQAPLAPSRNQENEGFNRIGFLLKTINNINKLPVAFNIITERAKEEIHNIIVKSTESIRSKHPSLLKMATSLKNDNHFGLPVQDILSIILRECFWEIFLKLLYAIQCHRAIFEMSNILQPTSSAKPAFKFNKIWGKLLDEIELLLVRYINDPELISSNNGSIKPINGATNNAPTLPKRKNPKIFSLEYNIEDNSSVKDQAFELKALLKDIFPGFSVSSNMDLDSIYVKDESFEQDEPLVPPSVFNMKVILDPFLLFTQSTSTIVPSVLTQNTISSLTFFDDYMNKSFLPKIQMTMDYLFTVEVESNNPYALELSDENHNIFKTALDFQRLFYNLLNVFNTANTFREKISYCILDLLNHFYNYYLGLFNSLIGTSDRHLTRKIITAWLQNGILMDQEQKILNGDETLFHEESIELFKEIPHFYQAGKGLSKSDLFNNLTLDTILQFSASVLWILNWLPGLKKAINIDEVSQEPMLDADRLRSSWTFSESMDLNYSNPSSSPNSLGNLKILLDDKASKKFDETIDGFKTLKFKLITILRFNIRALCIYDIGSFFQNTKIWNMDVGSIELDQNIASLISELRRTESKLKQQLPEKEKNSIFIGLDIVNNYALIKGAKSIKVLNHNGIKKMLRNVNVLQHAYRNLSSEPSKINMNVTMNFYSLCGSSEAELFEYIKDNELPHCSVEDLKTILRLQFSEEMHRQLKRQSTSSTKGSIKPSNKRYTEALEKLSNLEKEQSKEGARTKIGKLKSKLNAVHTANEK 96FC3139850C07F3 sec10_yeast Exocyst complex component SEC10 SEC10 L9362.12 YLR166C protein protein yeast Saccharomyces cerevisiae Baker's yeast MNSLYELDPKWKKLLKTDNFLGGLTVNEFVQELSKDHRNDVLIDANTKNLPTNEKDQDAIREAIWKQLDPKPYIRTFESTLKELKNLNEETLNKRQYFSEQVATQEVIHSENVIKLSKDLHTTLLTFDKLDDRLTNVTQVVSPLGDKLETAIKKKQNYIQSVELIRRYNDFYSMGKSDIVEQLRLSKNWKLNLKSVKLMKNLLILSSKLETSSIPKTINTKLVIEKYSEMMENELLENFNSAYRENNFTKLNEIAIILNNFNGGVNVIQSFINQHDYFIDTKQIDLENEFENVFIKNVKFKEQLIDFENHSVIIETSMQNLINDVETVIKNESKIVKRVFEEKATHVIQLFIQRVFAQKIEPRFEVLLRNSLSISNLAYVRILHGLFTLFGKFTKSLIDYFQLLEIDDSNQILSTTLEQCFADLFSHYLYDRSKYFGIEKRSLEAILVDMTSKFTVNYDKEINKRVLLDKYKEKLSTNVDAFMHSPRGNTHSRQDSTSRSKLSQFNSFLKTHLDKDHLSLNRTNTLSDSFNNSSSSTQYDVANNSSSLVNSSFTASDIDNSPNSPANYSLNDVDSMLKCVVESTARVMELIPNKAHLYILEILKIMFLGIVDSYMEIALEVAYWKICKVDINKTAGVVNLNFLKFISMSTEILDLLSISIKSIFLPLLNNSPEIKAQIIEMTNSQIQKMEILINIILQETITVISTKFSAILCKQKKKDFVPKSQELLDQDTLPAIEIVNILNLIFEQSSKFLKGKNLQTFLTLIGEELYGLLLSHYSHFQVNSIGGVVVTKDIIGYQTAIEDWGVASLIDKFATLRELANLFTVQPELLESLTKEGHLADIGRDIIQSYISNREDFNHDNFINSVKLNFR A32073099815388D exo70_yeast Exocyst complex component EXO70 EXO70 YJL085W J0932 Exocyst complex protein of 70 kDa protein protein yeast Saccharomyces cerevisiae Baker's yeast MPAEIDIDEADVLVLSQELQKTSKLTFEINKSLKKIAATSNQSSQLFTPILARNNVLTTLQRNIESTLNSVASVKDLANEASKYEIILQKGINQVGLKQYTQVVHKLDDMLEDIQSGQANREENSEFHGILTHLEQLIKRSEAQLRVYFISILNSIKPFDPQINITKKMPFPYYEDQQLGALSWILDYFHGNSEGSIIQDILVGERSKLILKCMAFLEPFAKEISTAKNAPYEKGSSGMNSYTEALLGFIANEKSLVDDLYSQYTESKPHVLSQILSPLISAYAKLFGANLKIVRSNLENFGFFSFELVESINDVKKSLRGKELQNYNLLQDCTQEVRQVTQSLFRDAIDRIIKKANSISTIPSNNGVTEATVDTMSRLRKFSEYKNGCLGAMDNITRENWLPSNYKEKEYTLQNEALNWEDHNVLLSCFISDCIDTLAVNLERKAQIALMPNQEPDVANPNSSKNKHKQRIGFFILMNLTLVEQIVEKSELNLMLAGEGHSRLERLKKRYISYMVSDWRDLTANLMDSVFIDSSGKKSKDKEQIKEKFRKFNEGFEDLVSKTKQYKLSDPSLKVTLKSEIISLVMPMYERFYSRYKDSFKNPRKHIKYTPDELTTVLNQLVR 277D89176FC2837D sec6p-sec8p-1 3144191 N/A 3144194 unspecified role unspecified role bait bait MBP Tag MBP Tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3144195 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false sec6p-sec10p-1 3144192 N/A 3144196 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3144194 unspecified role unspecified role bait bait binding region (411-805) binding region (411-805) sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false exo70p-sec6p-2 3144193 N/A 3144197 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3144194 unspecified role unspecified role prey prey binding region (411-805) binding region (411-805) sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false MBP Tag MBP Tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false