DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ kikuchi-2006-1 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kikuchi H., Almer G., Yamashita S., Guégan C., Nagai M., Xu Z., Sosunov AA., McKhann GM., Przedborski S. DIP original experiment: DIP-262827X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Spinal cord endoplasmic reticulum stress associated with a microsomal accumulation of mutant superoxide dismutase-1 in an ALS model. Kikuchi H., Almer G., Yamashita S., Guégan C., Nagai M., Xu Z., Sosunov AA., McKhann GM., Przedborski S. Only protein-protein interactions imex curation kikuchi-2006-2 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Kikuchi H., Almer G., Yamashita S., Guégan C., Nagai M., Xu Z., Sosunov AA., McKhann GM., Przedborski S. DIP original experiment: DIP-262828X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Spinal cord endoplasmic reticulum stress associated with a microsomal accumulation of mutant superoxide dismutase-1 in an ALS model. Kikuchi H., Almer G., Yamashita S., Guégan C., Nagai M., Xu Z., Sosunov AA., McKhann GM., Przedborski S. Only protein-protein interactions imex curation kikuchi-2006-3 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kikuchi H., Almer G., Yamashita S., Guégan C., Nagai M., Xu Z., Sosunov AA., McKhann GM., Przedborski S. DIP original experiment: DIP-262830X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Spinal cord endoplasmic reticulum stress associated with a microsomal accumulation of mutant superoxide dismutase-1 in an ALS model. Kikuchi H., Almer G., Yamashita S., Guégan C., Nagai M., Xu Z., Sosunov AA., McKhann GM., Przedborski S. Only protein-protein interactions imex curation kikuchi-2006-4 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kikuchi H., Almer G., Yamashita S., Guégan C., Nagai M., Xu Z., Sosunov AA., McKhann GM., Przedborski S. DIP original experiment: DIP-262829X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Spinal cord endoplasmic reticulum stress associated with a microsomal accumulation of mutant superoxide dismutase-1 in an ALS model. Kikuchi H., Almer G., Yamashita S., Guégan C., Nagai M., Xu Z., Sosunov AA., McKhann GM., Przedborski S. Only protein-protein interactions imex curation kikuchi-2006-5 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Kikuchi H., Almer G., Yamashita S., Guégan C., Nagai M., Xu Z., Sosunov AA., McKhann GM., Przedborski S. DIP original experiment: DIP-262831X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Spinal cord endoplasmic reticulum stress associated with a microsomal accumulation of mutant superoxide dismutase-1 in an ALS model. Kikuchi H., Almer G., Yamashita S., Guégan C., Nagai M., Xu Z., Sosunov AA., McKhann GM., Przedborski S. Only protein-protein interactions imex curation bip_mouse Endoplasmic reticulum chaperone BiP Immunoglobulin heavy chain-binding protein Hspa5 Grp78 78 kDa glucose-regulated protein Binding-immunoglobulin protein Heat shock protein 70 family protein 5 Heat shock protein family A member 5 protein protein mouse Mus musculus Mouse MMKFTVVAAALLLLGAVRAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSGGPPPTGEEDTSEKDEL sodc_human Superoxide dismutase [Cu-Zn] Superoxide dismutase 1 SOD1 protein protein human Homo sapiens Human MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ atf6b_mouse Cyclic AMP-dependent transcription factor ATF-6 beta Crebl1 Activating transcription factor 6 beta cAMP-responsive element-binding protein-like 1 cAMP response element-binding protein-related protein Atf6b protein protein mouse Mus musculus Mouse MAELMLLSEIADPTRFFTDNLLSPEDWDSTLYSGLDEVAEEQAQLFRCVEQDVPFDSSSLDVGMDVSPPEPPWDPLPIFPDLQVKSEPSSPCSSSSLSSESSHLSTEPPSQVPGVGEVLHVKMESLAPPLCLLGDDPASPFETVQITVGSASDDLSDIQTKLEPASPSSSVHSEASLLSADSPSQPFIGEEVLEVKTESPSPPGCLLWDVPASSLGAVQISMGPSPDSSSGKAPATRKPPLQPKPVVLTTVPVPPRAGPTSAAVLLQPLVQQPAVSPVVLIQGAIRVQPEGPAPAAPRPERKSIVPAPMPGNSCPPEVDAKLLKRQQRMIKNRESACQSRRKKKEYLQGLEARLQAVLADNQQLRRENAALRRRLEALLAENSGLKLGSGNRKVVCIMVFLLFIAFNFGPVSISEPPPAPMSPRMSREEPRPQRHLLGFSEPGPAHGMEPLREAAQSPGEQQPSSAGRPSFRNLTAFPGGAKELLLRDLDQLFLSSDCRHFNRTESLRLADELSGWVQRHQRGRRKIPHRAQERQKSQLRKKSPPVKPVPTQPPGPPERDPVGQLQLYRHPGRSQPEFLDAIDRREDTFYVVSFRRDHLLLPAISHNKTSRPKMSLVMPAMAPNETVSGRGPPGDYEEMMQIECEVMDTRVIHIKTSTVPPSLRKQPSPSPGNTTGGPLPGSAASPAHQASQPLYLNHP mint Transcriptional factor that acts in the unfolded protein response (UPR) pathway by activating UPR target genes induced during ER stress. Binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) (5'-CCAATN(9)CCAC[GA]-3') when NF-Y is bound to ERSE sod1-hspa5-1 3143251 N/A 3143256 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3143257 unspecified role unspecified role bait bait P00441:p.Gly94Ala G94A mutation increasing strength mutation increasing interaction strength mutation increasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false mouse Mus musculus Mouse physical association physical association false false false hspa5-sod1-1 3143252 N/A 3143256 unspecified role unspecified role bait bait mouse Mus musculus Mouse N/A 3143257 unspecified role unspecified role prey prey P00441:p.Gly94Ala G94A mutation increasing strength mutation increasing interaction strength mutation increasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false mouse Mus musculus Mouse physical association physical association false false false hspa5-atf6b-1 3143253 N/A 3143258 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3143256 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association false false false hspa5-atf6b-2 3143254 N/A 3143258 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3143256 unspecified role unspecified role bait bait mouse Mus musculus Mouse N/A 3143257 unspecified role unspecified role prey prey P00441:p.Gly94Ala G94A mutation mutation certain certain sequence position certain certain certain sequence position certain false mouse Mus musculus Mouse association association false false false hspa5-sod1-2 3143255 N/A 3143256 unspecified role unspecified role bait bait Region 391-636 Region 391-636 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-509 Region 1-509 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3143257 unspecified role unspecified role prey prey P00441:p.Gly94Ala G94A mutation mutation certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false Deletion of region 391-509 decreased the interaction rate/strength.