DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ kanelis-2006-1 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Kanelis V., Bruce MC., Skrynnikov NR., Rotin D., Forman-Kay JD. DIP original experiment: DIP-66164X Structure (London, England : 1993) 2006 IMEx Structural determinants for high-affinity binding in a Nedd4 WW3* domain-Comm PY motif complex. Kanelis V., Bruce MC., Skrynnikov NR., Rotin D., Forman-Kay JD. Only protein-protein interactions imex curation kanelis-2006-2 in vitro In vitro fluorescence fluorescence technology fluorescence predetermined predetermined participant predetermined Kanelis V., Bruce MC., Skrynnikov NR., Rotin D., Forman-Kay JD. DIP original experiment: DIP-66165X Structure (London, England : 1993) 2006 IMEx Structural determinants for high-affinity binding in a Nedd4 WW3* domain-Comm PY motif complex. Kanelis V., Bruce MC., Skrynnikov NR., Rotin D., Forman-Kay JD. Only protein-protein interactions imex curation kanelis-2006-3 in vitro In vitro fluorescence fluorescence technology fluorescence predetermined predetermined participant predetermined Kanelis V., Bruce MC., Skrynnikov NR., Rotin D., Forman-Kay JD. DIP original experiment: DIP-66166X Structure (London, England : 1993) 2006 IMEx Structural determinants for high-affinity binding in a Nedd4 WW3* domain-Comm PY motif complex. Kanelis V., Bruce MC., Skrynnikov NR., Rotin D., Forman-Kay JD. Only protein-protein interactions imex curation comm_drome Protein commissureless 1 comm Protein commissureless CG17943 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MISTTDYPTVETTTTAEELYAEYISAPASSMSPAAIAEHLQQNQITFEIPSAHDLRHIDALNSFNALLQRIGNAAVSYDPAPPSGWSPDGSISTEQLSKSVVLDLADLRDRSEESGESSWWSQIFGDADMHVIINYLWIGVVSSLVILSLVFILFSCYFYRKFRTWKKCNKDIRAQIHAASDSYSSHLVGCDASRLLLHQQMQHPHHRSSEAGFYQIESPPCYTIATGLPSYDEALHHQPRHFAYGMKFVYPSLAAVHHHHHCISNWEKQEPLNKLQKCKLSAAAAVEEDKADSSSSTSASASPSSSESSNLATATPAICINMPSGRQDEEVDNSDSDSAIAVAVAVAQSLQPAAPADDDCASLVVVVAA nedd4_drome E3 ubiquitin-protein ligase Nedd-4 HECT-type E3 ubiquitin transferase NEDD4 CG7555 Nedd4 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSARSSGLVAAAALPVPSSSSSVAGGDVPRPPPRRRAASVAGQQQTRQEFGNGYTPRRSLAAVNDSGDSCHLRIVVLTGQSLAKKDIFGASDPYVRIDLNTINGDINIDSVLTKTKKKTLNPTWNEEFIFRVKPSEHKLVFQVFDENRLTRDDFLGMVELTLVNLPTEQEGRTIGEQSYTLRPRRSVGAKSRIKGTLRIYHAFIRETREQSEPSSGNSDGEWEHVEATNAGETSAQPHPFPTGGHDALPAGWEERQDANGRTYYVNHTARTTQWDRPTVLNSHSSQSTDDQLASDFQRRFHISVDDTESGRSADSISHNSIEDNNNAAGLAYTPKTAATSSAPPNTPTNNNGILAQIAMQYRAEEDQDPTVDHTSFVYNSLRHPVAHRQPEISATSLQNDLRPVREAPGVPDIAITNPFTRRAAGNMAGGAGWQQERRRQQMQLHIQQHQQRQQQQQQNRILLDVDHRQQEPQHRGQRHQQQHRPSNEDTDHTDSHNPSDISAPSTRRNSEEDNAAVPPMEQNTGGEEEPLPPRWSMQVAPNGRTFFIDHASRRTTWIDPRNGRASPMPNQTRRVEDDLGPLPEGWEERVHTDGRVFYIDHNTRTTQWEDPRLSNPNIAGQAVPYSRDYKQKYEYFKSHIRKPTNVPNKFEIRIRRTSILEDSYRIISSVTKTDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNYTLQINNGSGLCNEEHLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDMESVDTEYYNSLMWIKENDPRILELTFCLDEDVFGQKSQHELKPGGANIDVTNENKDEYIKLVIEWRFVARVKEQMSSFLDGFGSIIPLNLIKIFDEHELELLMCGIQNIDVKDWRENTLYKGDYHMNHIIIQWFWRAVLSFSNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQMFTIEKWGTPNNFPRAHTCFNRLDLPPYEGYLQLKDKLIKAIEGSQGFAGVD comm-nedd4-1 3142245 N/A 3142248 unspecified role unspecified role unspecified role unspecified role Binding Region 227-237 Binding Region 227-237 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3142249 unspecified role unspecified role unspecified role unspecified role Binding Region 526-566 Binding Region 526-566 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 13C Label 13C Label 13c label 13c label C13 13C C13 13C nmr nuclear magnetic resonance NMR nmr NMR n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 15N Label 15N Label 15n label 15n label 15N N15 15N N15 nmr nuclear magnetic resonance NMR nmr NMR n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false comm-nedd4-2 3142246 N/A 3142248 unspecified role unspecified role unspecified role unspecified role Binding Region 227-237 Binding Region 227-237 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3142249 unspecified role unspecified role unspecified role unspecified role Binding Region 527-571 Binding Region 527-571 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false comm-nedd4-3 3142247 N/A 3142248 unspecified role unspecified role unspecified role unspecified role Region 227-237 Region 227-237 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3142249 unspecified role unspecified role unspecified role unspecified role Region 577-612 Region 577-612 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false