DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ sheng-2006-1 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. DIP original experiment: DIP-66187X Nature structural & molecular biology 2006 IMEx Molecular recognition of p53 and MDM2 by USP7/HAUSP. Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. Only protein-protein interactions imex curation sheng-2006-2 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. DIP original experiment: DIP-66191X Nature structural & molecular biology 2006 IMEx Molecular recognition of p53 and MDM2 by USP7/HAUSP. Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. Only protein-protein interactions imex curation sheng-2006-3 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. DIP original experiment: DIP-66182X Nature structural & molecular biology 2006 IMEx Molecular recognition of p53 and MDM2 by USP7/HAUSP. Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. Only protein-protein interactions imex curation sheng-2006-4 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. DIP original experiment: DIP-66184X Nature structural & molecular biology 2006 IMEx Molecular recognition of p53 and MDM2 by USP7/HAUSP. Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. Only protein-protein interactions imex curation sheng-2006-5 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. DIP original experiment: DIP-66189X Nature structural & molecular biology 2006 IMEx Molecular recognition of p53 and MDM2 by USP7/HAUSP. Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. Only protein-protein interactions imex curation sheng-2006-6 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. DIP original experiment: DIP-66190X Nature structural & molecular biology 2006 IMEx Molecular recognition of p53 and MDM2 by USP7/HAUSP. Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. Only protein-protein interactions imex curation sheng-2006-7 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. DIP original experiment: DIP-66193X Nature structural & molecular biology 2006 IMEx Molecular recognition of p53 and MDM2 by USP7/HAUSP. Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. Only protein-protein interactions imex curation sheng-2006-8 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. DIP original experiment: DIP-66194X Nature structural & molecular biology 2006 IMEx Molecular recognition of p53 and MDM2 by USP7/HAUSP. Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. Only protein-protein interactions imex curation sheng-2006-9 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. DIP original experiment: DIP-66185X Nature structural & molecular biology 2006 IMEx Molecular recognition of p53 and MDM2 by USP7/HAUSP. Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. Only protein-protein interactions imex curation sheng-2006-10 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. DIP original experiment: DIP-66186X Nature structural & molecular biology 2006 IMEx Molecular recognition of p53 and MDM2 by USP7/HAUSP. Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. Only protein-protein interactions imex curation sheng-2006-11 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. DIP original experiment: DIP-66183X Nature structural & molecular biology 2006 IMEx Molecular recognition of p53 and MDM2 by USP7/HAUSP. Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. Only protein-protein interactions imex curation sheng-2006-12 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. DIP original experiment: DIP-66188X Nature structural & molecular biology 2006 IMEx Molecular recognition of p53 and MDM2 by USP7/HAUSP. Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. Only protein-protein interactions imex curation sheng-2006-13 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. DIP original experiment: DIP-66192X Nature structural & molecular biology 2006 IMEx Molecular recognition of p53 and MDM2 by USP7/HAUSP. Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. Only protein-protein interactions imex curation sheng-2006-14 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. DIP original experiment: DIP-217317X Nature structural & molecular biology 2006 IMEx Molecular recognition of p53 and MDM2 by USP7/HAUSP. Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. Only protein-protein interactions imex curation sheng-2006-15 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. DIP original experiment: DIP-217318X Nature structural & molecular biology 2006 IMEx Molecular recognition of p53 and MDM2 by USP7/HAUSP. Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. Only protein-protein interactions imex curation sheng-2006-16 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. DIP original experiment: DIP-217319X Nature structural & molecular biology 2006 IMEx Molecular recognition of p53 and MDM2 by USP7/HAUSP. Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. Only protein-protein interactions imex curation sheng-2006-17 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. DIP original experiment: DIP-217320X Nature structural & molecular biology 2006 IMEx Molecular recognition of p53 and MDM2 by USP7/HAUSP. Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. Only protein-protein interactions imex curation sheng-2006-18 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. DIP original experiment: DIP-217321X Nature structural & molecular biology 2006 IMEx Molecular recognition of p53 and MDM2 by USP7/HAUSP. Sheng Y., Saridakis V., Sarkari F., Duan S., Wu T., Arrowsmith CH., Frappier L. Only protein-protein interactions imex curation ebna1_ebvb9 Epstein-Barr nuclear antigen 1 EBNA1 BKRF1 protein protein ebvb9 Epstein-Barr virus (strain B95-8) (HHV-4) Human herpesvirus 4 HHV-4 MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGRGRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAGAGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGGGAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAGGGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRGGSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPPPGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDEGEEGQE 4D161653E16FC341 ubp7_human Ubiquitin carboxyl-terminal hydrolase 7 USP7 HAUSP Ubiquitin thioesterase 7 Ubiquitin-specific-processing protease 7 Deubiquitinating enzyme 7 Herpesvirus-associated ubiquitin-specific protease protein protein human Homo sapiens Human MNHQQQQQQQKAGEQQLSEPEDMEMEAGDTDDPPRITQNPVINGNVALSDGHNTAEEDMEDDTSWRSEATFQFTVERFSRLSESVLSPPCFVRNLPWKIMVMPRFYPDRPHQKSVGFFLQCNAESDSTSWSCHAQAVLKIINYRDDEKSFSRRISHLFFHKENDWGFSNFMAWSEVTDPEKGFIDDDKVTFEVFVQADAPHGVAWDSKKHTGYVGLKNQGATCYMNSLLQTLFFTNQLRKAVYMMPTEGDDSSKSVPLALQRVFYELQHSDKPVGTKKLTKSFGWETLDSFMQHDVQELCRVLLDNVENKMKGTCVEGTIPKLFRGKMVSYIQCKEVDYRSDRREDYYDIQLSIKGKKNIFESFVDYVAVEQLDGDNKYDAGEHGLQEAEKGVKFLTLPPVLHLQLMRFMYDPQTDQNIKINDRFEFPEQLPLDEFLQKTDPKDPANYILHAVLVHSGDNHGGHYVVYLNPKGDGKWCKFDDDVVSRCTKEEAIEHNYGGHDDDLSVRHCTNAYMLVYIRESKLSEVLQAVTDHDIPQQLVERLQEEKRIEAQKRKERQEAHLYMQVQIVAEDQFCGHQGNDMYDEEKVKYTVFKVLKNSSLAEFVQSLSQTMGFPQDQIRLWPMQARSNGTKRPAMLDNEADGNKTMIELSDNENPWTIFLETVDPELAASGATLPKFDKDHDVMLFLKMYDPKTRSLNYCGHIYTPISCKIRDLLPVMCDRAGFIQDTSLILYEEVKPNLTERIQDYDVSLDKALDELMDGDIIVFQKDDPENDNSELPTAKEYFRDLYHRVDVIFCDKTIPNDPGFVVTLSNRMNYFQVAKTVAQRLNTDPMLLQFFKSQGYRDGPGNPLRHNYEGTLRDLLQFFKPRQPKKLYYQQLKMKITDFENRRSFKCIWLNSQFREEEITLYPDKHGCVRDLLEECKKAVELGEKASGKLRLLEIVSYKIIGVHQEDELLECLSPATSRTFRIEEIPLDQVDIDKENEMLVTVAHFHKEVFGTFGIPFLLRIHQGEHFREVMKRIQSLLDIQEKEFEKFKFAIVMMGRHQYINEDEYEVNLKDFEPQPGNMSHPRPWLGLDHFNKAPKRSRYTYLEKAIKIHN p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP ebna1-usp7-2 3141411 N/A 3141429 unspecified role unspecified role bait bait 395-450 binding region 395-450 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag full dna sequence full identification by DNA sequencing full dna sequence n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3141430 unspecified role unspecified role prey prey 62-205 binding region 62-205 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ebna1-usp7-3 3141412 N/A 3141429 unspecified role unspecified role neutral component neutral component 441-450 binding region 441-450 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3141430 unspecified role unspecified role neutral component neutral component 1-205 binding region 1-205 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tp53-usp7-8 3141413 N/A 3141431 unspecified role unspecified role bait bait 356-365 binding region 356-365 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag full dna sequence full identification by DNA sequencing full dna sequence n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3141430 unspecified role unspecified role prey prey 62-205 binding region 62-205 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tp53-usp7-9 3141414 N/A 3141431 unspecified role unspecified role neutral component neutral component 358-367 binding region 358-367 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3141430 unspecified role unspecified role neutral component neutral component 62-205 binding region 62-205 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tp53-usp7-10 3141415 N/A 3141431 unspecified role unspecified role neutral component neutral component 356-365 binding region 356-365 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3141430 unspecified role unspecified role neutral component neutral component gion gion sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false mdm2-usp7-5 3141416 N/A 3141432 unspecified role unspecified role neutral component neutral component 143-150 binding region 143-150 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3141430 unspecified role unspecified role neutral component neutral component 1-205 binding region 1-205 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false mdm2-usp7-6 3141417 N/A 3141432 unspecified role unspecified role neutral component neutral component 141-150 binding region 141-150 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3141430 unspecified role unspecified role neutral component neutral component 54-205 binding region 54-205 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ebna1-usp7-4 3141418 N/A 3141429 unspecified role unspecified role neutral component neutral component 441-450 binding region 441-450 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3141430 unspecified role unspecified role neutral component neutral component 1-205 binding region 1-205 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false mdm2-usp7-7 3141419 N/A 3141432 unspecified role unspecified role unspecified role unspecified role 1-160 binding region 1-160 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag full dna sequence full identification by DNA sequencing full dna sequence n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3141430 unspecified role unspecified role unspecified role unspecified role 62-205 binding region 62-205 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false mdm2-usp7-8 3141420 N/A 3141432 unspecified role unspecified role unspecified role unspecified role 1-160 binding region 1-160 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag full dna sequence full identification by DNA sequencing full dna sequence n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3141430 unspecified role unspecified role unspecified role unspecified role 62-205 binding region 62-205 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tp53-usp7-11 3141421 N/A 3141431 unspecified role unspecified role neutral component neutral component 358-367 binding region 358-367 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3141430 unspecified role unspecified role neutral component neutral component 54-205 binding region 54-205 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ebna1-usp7-5 3141422 N/A 3141429 unspecified role unspecified role neutral component neutral component 395-450 binding region 395-450 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3141430 unspecified role unspecified role neutral component neutral component 62-205 binding region 62-205 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false mdm2-usp7-9 3141423 N/A 3141432 unspecified role unspecified role neutral component neutral component 152-161 binding region 152-161 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3141430 unspecified role unspecified role neutral component neutral component 1-205 binding region 1-205 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false mdm2-usp7-10 3141424 N/A 3141432 unspecified role unspecified role unspecified role unspecified role 1-160 binding region 1-160 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag full dna sequence full identification by DNA sequencing full dna sequence n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3141430 unspecified role unspecified role unspecified role unspecified role 62-205 binding region 62-205 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false 1 nodeMerge04 2011-10-21 mdm2-usp7-11 3141425 N/A 3141432 unspecified role unspecified role neutral component neutral component 143-150 binding region 143-150 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3141430 unspecified role unspecified role neutral component neutral component 1-205 binding region 1-205 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false 1 nodeMerge04 2011-10-21 mdm2-usp7-12 3141426 N/A 3141432 unspecified role unspecified role unspecified role unspecified role 1-160 binding region 1-160 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag full dna sequence full identification by DNA sequencing full dna sequence n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3141430 unspecified role unspecified role unspecified role unspecified role 62-205 binding region 62-205 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false 1 nodeMerge04 2011-10-21 mdm2-usp7-13 3141427 N/A 3141432 unspecified role unspecified role neutral component neutral component 152-161 binding region 152-161 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3141430 unspecified role unspecified role neutral component neutral component 1-205 binding region 1-205 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false 1 nodeMerge04 2011-10-21 mdm2-usp7-14 3141428 N/A 3141432 unspecified role unspecified role neutral component neutral component 141-150 binding region 141-150 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3141430 unspecified role unspecified role neutral component neutral component 54-205 binding region 54-205 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false 1 nodeMerge04 2011-10-21