DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ jozic-2005-1 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Jozic D., Cárdenes N., Deribe YL., Moncalián G., Hoeller D., Groemping Y., Dikic I., Rittinger K., Bravo J. DIP original experiment: DIP-255131X Nature structural & molecular biology 2005 IMEx Cbl promotes clustering of endocytic adaptor proteins. Jozic D., Cárdenes N., Deribe YL., Moncalián G., Hoeller D., Groemping Y., Dikic I., Rittinger K., Bravo J. Only protein-protein interactions imex curation jozic-2005-2 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Jozic D., Cárdenes N., Deribe YL., Moncalián G., Hoeller D., Groemping Y., Dikic I., Rittinger K., Bravo J. DIP original experiment: DIP-255129X Nature structural & molecular biology 2005 IMEx Cbl promotes clustering of endocytic adaptor proteins. Jozic D., Cárdenes N., Deribe YL., Moncalián G., Hoeller D., Groemping Y., Dikic I., Rittinger K., Bravo J. Only protein-protein interactions imex curation jozic-2005-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Jozic D., Cárdenes N., Deribe YL., Moncalián G., Hoeller D., Groemping Y., Dikic I., Rittinger K., Bravo J. DIP original experiment: DIP-255127X Nature structural & molecular biology 2005 IMEx Cbl promotes clustering of endocytic adaptor proteins. Jozic D., Cárdenes N., Deribe YL., Moncalián G., Hoeller D., Groemping Y., Dikic I., Rittinger K., Bravo J. Only protein-protein interactions imex curation jozic-2005-4 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Jozic D., Cárdenes N., Deribe YL., Moncalián G., Hoeller D., Groemping Y., Dikic I., Rittinger K., Bravo J. DIP original experiment: DIP-255133X Nature structural & molecular biology 2005 IMEx Cbl promotes clustering of endocytic adaptor proteins. Jozic D., Cárdenes N., Deribe YL., Moncalián G., Hoeller D., Groemping Y., Dikic I., Rittinger K., Bravo J. Only protein-protein interactions imex curation jozic-2005-5 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Jozic D., Cárdenes N., Deribe YL., Moncalián G., Hoeller D., Groemping Y., Dikic I., Rittinger K., Bravo J. DIP original experiment: DIP-255128X Nature structural & molecular biology 2005 IMEx Cbl promotes clustering of endocytic adaptor proteins. Jozic D., Cárdenes N., Deribe YL., Moncalián G., Hoeller D., Groemping Y., Dikic I., Rittinger K., Bravo J. Only protein-protein interactions imex curation jozic-2005-6 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Jozic D., Cárdenes N., Deribe YL., Moncalián G., Hoeller D., Groemping Y., Dikic I., Rittinger K., Bravo J. DIP original experiment: DIP-255132X Nature structural & molecular biology 2005 IMEx Cbl promotes clustering of endocytic adaptor proteins. Jozic D., Cárdenes N., Deribe YL., Moncalián G., Hoeller D., Groemping Y., Dikic I., Rittinger K., Bravo J. Only protein-protein interactions imex curation jozic-2005-7 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Jozic D., Cárdenes N., Deribe YL., Moncalián G., Hoeller D., Groemping Y., Dikic I., Rittinger K., Bravo J. DIP original experiment: DIP-255130X Nature structural & molecular biology 2005 IMEx Cbl promotes clustering of endocytic adaptor proteins. Jozic D., Cárdenes N., Deribe YL., Moncalián G., Hoeller D., Groemping Y., Dikic I., Rittinger K., Bravo J. Only protein-protein interactions imex curation pak Pak peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) DATPPPVIAPRPEHTKSVYTR arhg7_rat Rho guanine nucleotide exchange factor 7 Arhgef7 Pak3bp Pixb Beta-Pix PAK-interacting exchange factor beta protein protein rat Rattus norvegicus (Rat) Rat MTDNANSQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTHNGRTGWFPSNYVREIKPSEKPVSPKSGTLKSPPKGFDTTAINKSYYNVVLQNILETEHEYSKELQSVLSTYLWPLQTSEKLSSANTSYLMGNLEEISSFQQVLVQSLEECTKSPEAQQRVGGCFLSLMPQMRTLYLAYCANHPSAVSVLTEHSEDLGEFMETKGASSPGILVLTTGLSKPFMRLDKYPTLLKELERHMEDYHPDRQDIQKSMTAFKNLSAQCQEVRKRKELELQILTEPIRSWEGDDIKTLGSVTYMSQVTIQCAGSEEKNERYLLLFPNLLLMLSASPRMSGFIYQGKLPTTGMTITKLEDSENHRNAFEISGSMIERILVSCNNQQDLHEWVEHLQRQTKVTSVSNPTIKPHSVPSHTLPSHPLTPSSKHADSKPVALTPAYHTLPHPSHHGTPHTTISWGPLEPPKTPKPWSLSCLRPAPPLRPSAALCYKEDLSRSPKTMKKLLPKRKPERKPSDEEFAVRKSTAALEEDAQILKVIEAYCTSAKTRQTLNSSSRKESAPQVLLPEEEKIIVEETKSNGQTVIEEKSLVDTVYALKDEVQELRQDNKKMKKSLEEEQRARKDLEKLVRKVLKNMNDPAWDETNL q13191-1 E3 ubiquitin-protein ligase CBL-B Casitas B-lineage lymphoma proto-oncogene b RING finger protein 56 RING-type E3 ubiquitin transferase CBL-B SH3-binding protein CBL-B Signal transduction protein CBL-B Nbla00127 CBLB RNF56 protein protein human Homo sapiens Human MANSMNGRNPGGRGGNPRKGRILGIIDAIQDAVGPPKQAAADRRTVEKTWKLMDKVVRLCQNPKLQLKNSPPYILDILPDTYQHLRLILSKYDDNQKLAQLSENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQDRRNLTKLSLIFSHMLAEIKAIFPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKVFRQCLHEVHQISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWGSILRNWNFLAVTHPGYMAFLTYDEVKARLQKYSTKPGSYIFRLSCTRLGQWAIGYVTGDGNILQTIPHNKPLFQALIDGSREGFYLYPDGRSYNPDLTGLCEPTPHDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTAWQESDGQGCPFCRCEIKGTEPIIVDPFDPRDEGSRCCSIIDPFGMPMLDLDDDDDREESLMMNRLANVRKCTDRQNSPVTSPGSSPLAQRRKPQPDPLQIPHLSLPPVPPRLDLIQKGIVRSPCGSPTGSPKSSPCMVRKQDKPLPAPPPPLRDPPPPPPERPPPIPPDNRLSRHIHHVESVPSRDPPMPLEAWCPRDVFGTNQLVGCRLLGEGSPKPGITASSNVNGRHSRVGSDPVLMRKHRRHDLPLEGAKVFSNGHLGSEEYDVPPRLSPPPPVTTLLPSIKCTGPLANSLSEKTRDPVEEDDDEYKIPSSHPVSLNSQPSHCHNVKPPVRSCDNGHCMLNGTHGPSSEKKSNIPDLSIYLKGDVFDSASDPVPLPPARPPTRDNPKHGSSLNRTPSDYDLLIPPLGEDAFDALPPSLPPPPPPARHSLIEHSKPPGSSSRPSSGQDLFLLPSDPFVDLASGQVPLPPARRLPGENVKTNRTSQDYDQLPSCSDGSQAPARPPKPRPRRTAPEIHHRKPHGPEAALENVDAKIAKLMGEGYAFEEVKRALEIAQNNVEVARSILREFAFPPPVSPRLNL q96b97-1 SH3 domain-containing kinase-binding protein 1 Human Src family kinase-binding protein 1 SH3KBP1 CD2-binding protein 3 CIN85 Cbl-interacting protein of 85 kDa protein protein human Homo sapiens Human MVEAIVEFDYQAQHDDELTISVGEIITNIRKEDGGWWEGQINGRRGLFPDNFVREIKKEMKKDPLTNKAPEKPLHEVPSGNSLLSSETILRTNKRGERRRRRCQVAFSYLPQNDDELELKVGDIIEVVGEVEEGWWEGVLNGKTGMFPSNFIKELSGESDELGISQDEQLSKSSLRETTGSESDGGDSSSTKSEGANGTVATAAIQPKKVKGVGFGDIFKDKPIKLRPRSIEVENDFLPVEKTIGKKLPATTATPDSSKTEMDSRTKSKDYCKVIFPYEAQNDDELTIKEGDIVTLINKDCIDVGWWEGELNGRRGVFPDNFVKLLPPDFEKEGNRPKKPPPPSAPVIKQGAGTTERKHEIKKIPPERPEMLPNRTEEKERPEREPKLDLQKPSVPAIPPKKPRPPKTNSLSRPGALPPRRPERPVGPLTHTRGDSPKIDLAGSSLSGILDKDLSDRSNDIDLEGFDSVVSSTEKLSHPTTSRPKATGRRPPSQSLTSSSLSSPDIFDSPSPEEDKEEHISLAHRGVDASKKTSKTVTISQVSDNKASLPPKPGTMAAGGGGPAPLSSAAPSPLSSSLGTAGHRANSPSLFGTEGKPKMEPAASSQAAVEELRTQVRELRSIIETMKDQQKREIKQLLSELDEEKKIRLRLQMEVNDIKKALQSK Interacts with CBL. mint arhgef7-pak-1 3139148 N/A 3139155 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3139156 unspecified role unspecified role unspecified role unspecified role Region 5-66 Region 5-66 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false arhgef7-cblb-1 3139149 N/A 3139156 unspecified role unspecified role unspecified role unspecified role Region 5-66 Region 5-66 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3139157 unspecified role unspecified role unspecified role unspecified role Region 899-914 Region 899-914 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q13191-1:p.Arg904Ala R904A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false cblb-sh3kbp1-16 3139150 N/A 3139158 unspecified role unspecified role unspecified role unspecified role Region 1-58 Region 1-58 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3139157 unspecified role unspecified role unspecified role unspecified role Region 902-912 Region 902-912 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false sh3kbp1-cblb-8 3139151 N/A 3139158 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3139158 unspecified role unspecified role bait bait Region 1-328 Region 1-328 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human Homo sapiens Human N/A 3139157 unspecified role unspecified role prey prey human Homo sapiens Human association association false false false arhgef7-cblb-2 3139152 N/A 3139156 unspecified role unspecified role unspecified role unspecified role Region 5-66 Region 5-66 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3139157 unspecified role unspecified role unspecified role unspecified role Region 899-914 Region 899-914 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false pak-sh3kbp1-1 3139153 N/A 3139158 unspecified role unspecified role unspecified role unspecified role Region 1-58 Region 1-58 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3139155 unspecified role unspecified role unspecified role unspecified role in vitro In vitro direct interaction direct interaction false false false cblb-sh3kbp1-17 3139154 N/A 3139158 unspecified role unspecified role unspecified role unspecified role Region 1-58 Region 1-58 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3139157 unspecified role unspecified role unspecified role unspecified role Region 899-914 Region 899-914 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false