DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ khan-2005-1 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Khan JA., Dunn BM., Tong L. DIP original experiment: DIP-132851X Structure (London, England : 1993) 2005 IMEx Crystal structure of human Taspase1, a crucial protease regulating the function of MLL. Khan JA., Dunn BM., Tong L. Only protein-protein interactions imex curation khan-2005-2 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Khan JA., Dunn BM., Tong L. DIP original experiment: DIP-132856X Structure (London, England : 1993) 2005 IMEx Crystal structure of human Taspase1, a crucial protease regulating the function of MLL. Khan JA., Dunn BM., Tong L. Only protein-protein interactions imex curation khan-2005-3 ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Khan JA., Dunn BM., Tong L. DIP original experiment: DIP-132854X Structure (London, England : 1993) 2005 IMEx Crystal structure of human Taspase1, a crucial protease regulating the function of MLL. Khan JA., Dunn BM., Tong L. Only protein-protein interactions imex curation khan-2005-4 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Khan JA., Dunn BM., Tong L. DIP original experiment: DIP-132852X Structure (London, England : 1993) 2005 IMEx Crystal structure of human Taspase1, a crucial protease regulating the function of MLL. Khan JA., Dunn BM., Tong L. Only protein-protein interactions imex curation khan-2005-5 in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Khan JA., Dunn BM., Tong L. DIP original experiment: DIP-132857X Structure (London, England : 1993) 2005 IMEx Crystal structure of human Taspase1, a crucial protease regulating the function of MLL. Khan JA., Dunn BM., Tong L. Only protein-protein interactions imex curation khan-2005-6 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Khan JA., Dunn BM., Tong L. DIP original experiment: DIP-132855X Structure (London, England : 1993) 2005 IMEx Crystal structure of human Taspase1, a crucial protease regulating the function of MLL. Khan JA., Dunn BM., Tong L. Only protein-protein interactions imex curation khan-2005-7 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Khan JA., Dunn BM., Tong L. DIP original experiment: DIP-132853X Structure (London, England : 1993) 2005 IMEx Crystal structure of human Taspase1, a crucial protease regulating the function of MLL. Khan JA., Dunn BM., Tong L. Only protein-protein interactions imex curation tasp1_human Threonine aspartase 1 TASP1 C20orf13 protein protein human Homo sapiens Human MTMEKGMSSGEGLPSRSSQVSAGKITAKELETKQSYKEKRGGFVLVHAGAGYHSESKAKEYKHVCKRACQKAIEKLQAGALATDAVTAALVELEDSPFTNAGMGSNLNLLGEIECDASIMDGKSLNFGAVGALSGIKNPVSVANRLLCEGQKGKLSAGRIPPCFLVGEGAYRWAVDHGIPSCPPNIMTTRFSLAAFKRNKRKLELAERVDTDFMQLKKRRQSSEKENDSGTLDTVGAVVVDHEGNVAAAVSSGGLALKHPGRVGQAALYGCGCWAENTGAHNPYSTAVSTSGCGEHLVRTILARECSHALQAEDAHQALLETMQNKFISSPFLASEDGVLGGVIVLRSCRCSAEPDSSQNKQTLLVEFLWSHTTESMCVGYMSAQDGKAKTHISRLPPGAVAGQSVAIEGGVCRLESPVN substrate Substrate peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) GKGQVDGADDK tasp1-1 3139092 N/A 3139099 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false tasp1-tasp1-1 3139093 N/A 3139099 unspecified role unspecified role prey prey Beta-subunit Region 234-420 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3139099 unspecified role unspecified role bait bait Alpha-subunit Region 1-206 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false tasp1-tasp1-2 3139094 N/A 3139099 unspecified role unspecified role unspecified role unspecified role Beta-subunit Region 234-420 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3139099 unspecified role unspecified role unspecified role unspecified role Alpha-subunit Region 1-206 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tasp1-tasp1-3 3139095 N/A 3139099 unspecified role unspecified role unspecified role unspecified role Beta-subunit Region 234-420 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3139099 unspecified role unspecified role unspecified role unspecified role Alpha-subunit Region 1-206 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false substrate-tasp1-1 3139096 N/A 3139100 enzyme target enzyme target substrate substrate unspecified role unspecified role in vitro In vitro N/A 3139099 enzyme enzyme unspecified role unspecified role Q9H6P5:p.Thr234Val T234V mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9H6P5:p.Thr234Ala T234A/S mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9H6P5:p.His47Ala H47A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9H6P5:p.Tyr61Ala Y61A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9H6P5:p.Asn100Ala N100A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9H6P5:p.Asp116Ala D116A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9H6P5:p.Ser252Ala S252A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9H6P5:p.Gln265Ala Q265A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9H6P5:p.Ser291Ala S291A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9H6P5:p.Thr234Ser mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli cleavage cleavage reaction cleavage false false false tasp1-2 3139097 N/A 3139099 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false tasp1-tasp1-4 3139098 N/A 3139099 unspecified role unspecified role unspecified role unspecified role Beta-subunit Region 234-420 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3139099 unspecified role unspecified role unspecified role unspecified role Alpha-subunit Region 1-206 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false