DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ schmitt-2005-1 in vitro In vitro ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Schmitt E., Panvert M., Blanquet S., Mechulam Y. DIP original experiment: DIP-132849X Structure (London, England : 1993) 2005 IMEx Structural basis for tRNA-dependent amidotransferase function. Schmitt E., Panvert M., Blanquet S., Mechulam Y. Only protein-protein interactions imex curation schmitt-2005-2 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Schmitt E., Panvert M., Blanquet S., Mechulam Y. DIP original experiment: DIP-132846X Structure (London, England : 1993) 2005 IMEx Structural basis for tRNA-dependent amidotransferase function. Schmitt E., Panvert M., Blanquet S., Mechulam Y. Only protein-protein interactions imex curation schmitt-2005-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Schmitt E., Panvert M., Blanquet S., Mechulam Y. DIP original experiment: DIP-132847X Structure (London, England : 1993) 2005 IMEx Structural basis for tRNA-dependent amidotransferase function. Schmitt E., Panvert M., Blanquet S., Mechulam Y. Only protein-protein interactions imex curation schmitt-2005-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Schmitt E., Panvert M., Blanquet S., Mechulam Y. DIP original experiment: DIP-132848X Structure (London, England : 1993) 2005 IMEx Structural basis for tRNA-dependent amidotransferase function. Schmitt E., Panvert M., Blanquet S., Mechulam Y. Only protein-protein interactions imex curation gate_pyrab Glutamyl-tRNA(Gln) amidotransferase subunit E gatE PAB1902 PYRAB06990 protein protein pyrab Pyrococcus abyssi (strain GE5 / Orsay) MMVMTDKFNYEELGLKVGLEIHRQLDTKKLFSPVPSELSDKVEFTFQRRLRPTMSELGEIDPAALEEFKKGRVYVYEGNYELTDLVYMDEEPPRGPDREALEVALQIAYLLNAKPVDEVYYMRKIVIDGSNVSGFQRTAIIATDGKVETPWGAVGIPTICLEEDAARIIERKDKEVIYRLDRLGIPLIEISTTPDIHHPEQAKVVAKFIGDALRATKKVKRGLGTIRQDLNVSIKGGARIEIKGVQELDMIPIIIEREVERQLNLLKIRDELRKRGVKPKDIKEEFYDVTDIFENTKSKIIARVIKKGGKVLAIKLPKFRGLIGREIQPGRRLGTEFADRAKKYVPGIFHIDELPNYGISQEEVNKVIERLNLSEEDAFVLVAAEEEKAKNALREVIKRAREAIEGVPEETRRALPDGNTEYMRPLPGKARMYPETDIPPLRIPDDLKKKIKENLPELPQAKVERYVKEYKLDRSLAQTLVDDERDELFEELVSMGVKPSLAASILVVVLKGLRKEVPIENVTDEHIREAFQLYLEGKIAKEAFEEIFKELARNPSKSAREVAEEKGLTLLSEEEVTRIIEEVIQQNIEVVKAKGMGAMGLIMGRVMAKVRGKADGKLVSQIVRRKLQEISGG 3F1BE701DBA6214A gatd_pyrab Glutamyl-tRNA(Gln) amidotransferase subunit D gatD PAB1901 PYRAB07000 protein protein pyrab Pyrococcus abyssi (strain GE5 / Orsay) MRVDEFLKERNINVGDFVRITKEEDGEEVTYEGYIMPPYELSAGDTLVLKLENGYNIGIALEKIRRIEVLERAKVKPEVHFEALIEGKPGLPEVTIIGTGGTIASRIDYETGAVYPAFTAEELAKAVPEIFEVANVKPKLLFNIFSEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSSDRPSSDAAMNLICSVRMATSEVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEEKVALVKVYPGISSEIIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMMLTNYAGEITPYTRFDTYLR 39C616E89496D61C gatd-gate-1 3139071 N/A 3139075 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3139076 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false gatd-2 3139072 N/A 3139076 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false gatd-gate-2 3139073 N/A 3139075 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3139076 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false gatd-gate-3 3139074 N/A 3139075 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3139076 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false