DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ mcmahon-2005-1 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined McMahon SA., Miller JL., Lawton JA., Kerkow DE., Hodes A., Marti-Renom MA., Doulatov S., Narayanan E., Sali A., Miller JF., Ghosh P. DIP original experiment: DIP-254797X Nature structural & molecular biology 2005 IMEx The C-type lectin fold as an evolutionary solution for massive sequence variation. McMahon SA., Miller JL., Lawton JA., Kerkow DE., Hodes A., Marti-Renom MA., Doulatov S., Narayanan E., Sali A., Miller JF., Ghosh P. Only protein-protein interactions imex curation mcmahon-2005-2 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie McMahon SA., Miller JL., Lawton JA., Kerkow DE., Hodes A., Marti-Renom MA., Doulatov S., Narayanan E., Sali A., Miller JF., Ghosh P. DIP original experiment: DIP-254798X Nature structural & molecular biology 2005 IMEx The C-type lectin fold as an evolutionary solution for massive sequence variation. McMahon SA., Miller JL., Lawton JA., Kerkow DE., Hodes A., Marti-Renom MA., Doulatov S., Narayanan E., Sali A., Miller JF., Ghosh P. Only protein-protein interactions imex curation mcmahon-2005-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined McMahon SA., Miller JL., Lawton JA., Kerkow DE., Hodes A., Marti-Renom MA., Doulatov S., Narayanan E., Sali A., Miller JF., Ghosh P. DIP original experiment: DIP-254800X Nature structural & molecular biology 2005 IMEx The C-type lectin fold as an evolutionary solution for massive sequence variation. McMahon SA., Miller JL., Lawton JA., Kerkow DE., Hodes A., Marti-Renom MA., Doulatov S., Narayanan E., Sali A., Miller JF., Ghosh P. Only protein-protein interactions imex curation mcmahon-2005-4 in vitro In vitro sls static light scattering sls predetermined predetermined participant predetermined McMahon SA., Miller JL., Lawton JA., Kerkow DE., Hodes A., Marti-Renom MA., Doulatov S., Narayanan E., Sali A., Miller JF., Ghosh P. DIP original experiment: DIP-254801X Nature structural & molecular biology 2005 IMEx The C-type lectin fold as an evolutionary solution for massive sequence variation. McMahon SA., Miller JL., Lawton JA., Kerkow DE., Hodes A., Marti-Renom MA., Doulatov S., Narayanan E., Sali A., Miller JF., Ghosh P. Only protein-protein interactions imex curation mcmahon-2005-5 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined McMahon SA., Miller JL., Lawton JA., Kerkow DE., Hodes A., Marti-Renom MA., Doulatov S., Narayanan E., Sali A., Miller JF., Ghosh P. DIP original experiment: DIP-254799X Nature structural & molecular biology 2005 IMEx The C-type lectin fold as an evolutionary solution for massive sequence variation. McMahon SA., Miller JL., Lawton JA., Kerkow DE., Hodes A., Marti-Renom MA., Doulatov S., Narayanan E., Sali A., Miller JF., Ghosh P. Only protein-protein interactions imex curation fibd_bpbpp Tail fiber receptor-binding protein Major tropism determinant protein mtd protein protein bpbpp Bordetella phage BPP-1 MSTAVQFRGGTTAQHATFTGAAREITVDTDKNTVVVHDGATAGGFPLARHDLVKTAFIKADKSAVAFTRTGNATASIKAGTIVEVNGKLVQFTADTAITMPALTAGTDYAIYVCDDGTVRADSNFSAPTGYTSTTARKVGGFHYAPGSNAAAQAGGNTTAQINEYSLWDIKFRPAALDPRGMTLVAGAFWADIYLLGVNHLTDGTSKYNVTIADGSASPKKSTKFGGDGSAAYSDGAWYNFAEVMTHHGKRLPNYNEFQALAFGTTEATSSGGTDVPTTGVNGTGATSAWNIFTSKWGVVQASGCLWTWGNEFGGVNGASEYTANTGGRGSVYAQPAAALFGGAWNGTSLSGSRAALWYSGPSFSFAFFGARGVCDHLILE pert_borbr Pertactin autotransporter P.94 BB1366 prn protein protein borbr Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) Alcaligenes bronchisepticus MNMSLSRIVKAAPLRRTTLAMALGALGALGAAPAAHADWNNQSIIKAGERQHGIHIKQSDGAGVRTATGTTIKVSGRQAQGVLLENPAAELRFQNGSVTSSGQLFDEGVRRFLGTVTVKAGKLVADHATLANVSDTRDDDGIALYVAGEQAQASIADSTLQGAGGVRVERGANVTVQRSTIVDGGLHIGTLQPLQPEDLPPSRVVLGDTSVTAVPASGAPAAVSVFGANELTVDGGHITGGRAAGVAAMDGAIVHLQRATIRRGDAPAGGAVPGGAVPGGAVPGGFGPLLDGWYGVDVSDSTVDLAQSIVEAPQLGAAIRAGRGARVTVSGGSLSAPHGNVIETGGGARRFPPPASPLSITLQAGARAQGRALLYRVLPEPVKLTLAGGAQGQGDIVATELPPIPGASSGPLDVALASQARWTGATRAVDSLSIDNATWVMTDNSNVGALRLASDGSVDFQQPAEAGRFKVLMVDTLAGSGLFRMNVFADLGLSDKLVVMRDASGQHRLWVRNSGSEPASANTMLLVQTPRGSAATFTLANKDGKVDIGTYRYRLAANGNGQWSLVGAKAPPAPKPAPQPGPQPGPQPPQPPQPPQRQPEAPAPQPPAGRELSAAANAAVNTGGVGLASTLWYAESNALSKRLGELRLNPDAGGAWGRGFAQRQQLDNRAGRRFDQKVAGFELGADHAVAVAGGRWHLGGLAGYTRGDRGFTGDGGGHTDSVHVGGYATYIANSGFYLDATLRASRLENDFKVAGSDGYAVKGKYRTHGVGASLEAGRRFAHADGWFLEPQAELAVFRVGGGAYRAANGLRVRDEGGSSVLGRLGLEVGKRIELAGGRQVQPYIKASVLQEFDGAGTVRTNGIAHRTELRGTRAELGLGMAAALGRGHSLYASYEYSKGPKLAMPWTFHAGYRYSW mtd-1 3138864 N/A 3138869 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false prn-mtd-1 3138865 N/A 3138870 unspecified role unspecified role bait bait Region 38-640 Region 38-640 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3138869 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false mtd-prn-1 3138866 N/A 3138870 unspecified role unspecified role unspecified role unspecified role Region 38-640 Region 38-640 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3138869 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false mtd-prn-2 3138867 N/A 3138870 unspecified role unspecified role unspecified role unspecified role Region 38-640 Region 38-640 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3138869 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false mtd-prn-3 3138868 N/A 3138870 unspecified role unspecified role unspecified role unspecified role Region 38-640 Region 38-640 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3138869 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false