UniProt UniProt http://www.uniprot.org zhu-2005-6 yeast Saccharomyces cerevisiae Baker's yeast affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Zhu Y., Fraering P., Vionnet C., Conzelmann A. andreas.conzelmann@unifr.ch Biochimica et biophysica acta 2005 imex curation Only protein-protein interactions imex curation zhu-2005-7 yeast Saccharomyces cerevisiae Baker's yeast pull down pull down western blot western blot Immuno blot Immuno blot Zhu Y., Fraering P., Vionnet C., Conzelmann A. andreas.conzelmann@unifr.ch Biochimica et biophysica acta 2005 imex curation Only protein-protein interactions imex curation gpi16_yeast GPI transamidase component GPI16 GPI16 YHR188C protein protein yeast Saccharomyces cerevisiae Baker's yeast MILTLAYFMLGTLLLGVFAEDTVSQIGINDSLWYPYDEALVLKPLPNNDLLLSFAFQLQSEPFDPAVSSMSYDAYEHYTTFPRAIPPLLESTATRQFHLRFTRGFWDALSWGQLPHAGKEAGASGVELWSQVQAMDQEQAFHNWKKLSNSLSGLFCSSLNFIDESRTTFPRRSYASDIGAPLFNSTEKLYLMRASLPNEPICTENLTPFIKLLPTRGKSGLTSLLDGHKLFDSLWNSISLDIATICSEDEDALCHYEMDARIEMVTHVPSALARGERPIPKPLDGNTLRCDTDKPFDSYQCFPLPEPSQTHFKLSQLFARPINNGNLFANRPTRICAEVDRSTWTAFLSVDDTIFSTHDNCFDLSNDQNEGGSGYDFILESTDTTKVTPIVPVPIHVSRSLTGNGQDRGGMRIVFHNDNDTPVKLIYFESLPWFMRVYLSSLQITSTTSPQLQENDIILDKYYLQAADRKRPGHLEFTMLIPANTDIVMTYQFDKALLQFAEYPPDANHGFEIDAAVITVLSLESSSSLYEMRTSTLLLSLSTPDFSMPYNVIILTSTIMGLIFGMLYNLMVKRMVTVEEADKITLQSGLKYKLLKLKEKFLGKKKTKTD E9613CE0EA99F963 gpi8_yeast GPI-anchor transamidase GPI8 D9798.2 YDR331W protein protein yeast Saccharomyces cerevisiae Baker's yeast MRIAMHLPLLLLYIFLLPLSGANNTDAAHEVIATNTNNWAVLVSTSRFWFNYRHMANVLSMYRTVKRLGIPDSQIILMLSDDVACNSRNLFPGSVFNNKDHAIDLYGDSVEVDYRGYEVTVENFIRLLTDRWTEDHPKSKRLLTDENSNIFIYMTGHGGDDFLKFQDAEEIASEDIADAFQQMYEKKRYNEIFFMIDTCQANTMYSKFYSPNILAVGSSEMDESSYSHHSDVEIGVAVIDRFTYYCLDFLEQIDKNSTLTLQDLFDSFTFEKIHSHVGVRTDLFDRNPSEVLITDFFANVQNVIPDDSKPLSVSHYHHYKDHIDTAQYELNNNVLDLALETYRKNNQSSKIEKKIKDIKSTSVLDVDIDSNECFFTSFKQSATIILALIVTILWFMLRGNTAKATYDLYTN gpi17_yeast GPI transamidase component GPI17 GPI17 D9461.20 YDR434W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNANLRKWVGFCFVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIPVYVKSDTYRFPDVHDAIQVQVNHLLNSQEQRVPWSLQVLPYNETIEQMESEGNQFHVVTLKLDEFIGYSSAYDTKETLVYYDDAAVLSNDLPFFVAQTLVEHTFQLEWTHLNKTCEGVSTNNDVAISYDPNIHLSVTLLSGDGNPVAWEIEPTLTDYFSPFRKFLSPLVNFTVDSSIVYHNDLNLHSLNGSCTSVTWFDLSHTIDLSELSSMAYYPEDSALNLAIVFPSASSSPDGLAFINGTRISDEITTLDWNSYLVPQWGVIIINKMPLKPNSVISEDYLEPMMYRFATDIFQLLGLTEGSQDLLSPYITIDSFKRLTILQNLDKATETLWSLVKLTQQFQGMSIPREVSDNVIEALDLRLQIIDLLNDPGKGGDIVWNNALHLSNELVKLCEKAFFNGEMVQQNFFPQEHMIAVYLPLLGPISAVMFFGFYNVMKEKNQKSKKNGTEREVAKEKLELKEAQKLHAIDGEDEL gpi17-gpi8-1 3314404 N/A 3314406 unspecified role unspecified role prey prey N/A 3314407 unspecified role unspecified role prey prey N/A 3314408 unspecified role unspecified role bait bait c_terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false association association false false false Fig. 3C gpi17-gpi16-1 3314405 N/A 3314407 unspecified role unspecified role prey prey N/A 3314408 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 3B