UniProt UniProt http://www.uniprot.org lahuna-2005-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lahuna O., Quellari M., Achard C., Nola S., Méduri G., Navarro C., Vitale N., Borg JP., Misrahi M. Binding sites for hScrib either according Audebert et al 2004 (PMID 15182672) or UniProt micheline.misrahi@bct.ap-hop-paris.fr The EMBO journal 2005 IMEx imex curation Only protein-protein interactions lahuna-2005-2 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Lahuna O., Quellari M., Achard C., Nola S., Méduri G., Navarro C., Vitale N., Borg JP., Misrahi M. Binding sites for hScrib either according Audebert et al 2004 (PMID 15182672) or UniProt micheline.misrahi@bct.ap-hop-paris.fr The EMBO journal 2005 IMEx imex curation Only protein-protein interactions lahuna-2005-3 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lahuna O., Quellari M., Achard C., Nola S., Méduri G., Navarro C., Vitale N., Borg JP., Misrahi M. Binding sites for hScrib either according Audebert et al 2004 (PMID 15182672) or UniProt micheline.misrahi@bct.ap-hop-paris.fr The EMBO journal 2005 imex curation Only protein-protein interactions lahuna-2005-4 rat-frtl_5 Rattus norvegicus thyroid cell line FRTL-5 frtl_5 Rat thyroid cell line FRTL-5 This thyroid cell line has been established from normal thyroid glands from 5 to 6-week-old Fischer rats. FRTL-5 is a clonal line of the continuously cultured FRTL cells derived by successive clonal isolation. The cells are characterised by maintaining highly differentiated thyroid features such as secretion of thyroglobulin and concentration of iodide. They display a sensitivity to thyroid-stimulating hormone (TSH)-induced increase in cAMP levels detecting TSH as absolute growth requirement. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lahuna O., Quellari M., Achard C., Nola S., Méduri G., Navarro C., Vitale N., Borg JP., Misrahi M. Binding sites for hScrib either according Audebert et al 2004 (PMID 15182672) or UniProt micheline.misrahi@bct.ap-hop-paris.fr The EMBO journal 2005 imex curation Only protein-protein interactions lahuna-2005-5 rat-thyroid rat thyroid gland Rat thyroid_gland confocal microscopy confocal microscopy immunostaining immunostaining Lahuna O., Quellari M., Achard C., Nola S., Méduri G., Navarro C., Vitale N., Borg JP., Misrahi M. Binding sites for hScrib either according Audebert et al 2004 (PMID 15182672) or UniProt micheline.misrahi@bct.ap-hop-paris.fr The EMBO journal 2005 imex curation Only protein-protein interactions lahuna-2005-6 canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line confocal microscopy confocal microscopy immunostaining immunostaining Lahuna O., Quellari M., Achard C., Nola S., Méduri G., Navarro C., Vitale N., Borg JP., Misrahi M. Binding sites for hScrib either according Audebert et al 2004 (PMID 15182672) or UniProt micheline.misrahi@bct.ap-hop-paris.fr The EMBO journal 2005 imex curation Only protein-protein interactions scrib_human Protein scribble homolog SCRIB CRIB1 KIAA0147 LAP4 SCRB1 VARTUL Protein LAP4 protein protein human Homo sapiens Human MLKCIPLWRCNRHVESVDKRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDVSRNDIPEIPESIKFCKALEIADFSGNPLSRLPDGFTQLRSLAHLALNDVSLQALPGDVGNLANLVTLELRENLLKSLPASLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENRLEELPAELGGLVLLTDLLLSQNLLRRLPDGIGQLKQLSILKVDQNRLCEVTEAIGDCENLSELILTENLLMALPRSLGKLTKLTNLNVDRNHLEALPPEIGGCVALSVLSLRDNRLAVLPPELAHTTELHVLDVAGNRLQSLPFALTHLNLKALWLAENQAQPMLRFQTEDDARTGEKVLTCYLLPQQPPPSLEDAGQQGSLSETWSDAPPSRVSVIQFLEAPIGDEDAEEAAAEKRGLQRRATPHPSELKVMKRSIEGRRSEACPCQPDSGSPLPAEEEKRLSAESGLSEDSRPSASTVSEAEPEGPSAEAQGGSQQEATTAGGEEDAEEDYQEPTVHFAEDALLPGDDREIEEGQPEAPWTLPGGRQRLIRKDTPHYKKHFKISKLPQPEAVVALLQGMQPDGEGPVAPGGWHNGPHAPWAPRAQKEEEEEEEGSPQEEEVEEEEENRAEEEEASTEEEDKEGAVVSAPSVKGVSFDQANNLLIEPARIEEEELTLTILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVALQGAEHHEAVEALRGAGTAVQMRVWRERMVEPENAVTITPLRPEDDYSPRERRGGGLRLPLLPPESPGPLRQRHVACLARSERGLGFSIAGGKGSTPYRAGDAGIFVSRIAEGGAAHRAGTLQVGDRVLSINGVDVTEARHDHAVSLLTAASPTIALLLEREAGGPLPPSPLPHSSPPTAAVATTSITTATPGVPGLPSLAPSLLAAALEGPYPVEEIRLPRAGGPLGLSIVGGSDHSSHPFGVQEPGVFISKVLPRGLAARSGLRVGDRILAVNGQDVRDATHQEAVSALLRPCLELSLLVRRDPAPPGLRELCIQKAPGERLGISIRGGARGHAGNPRDPTDEGIFISKVSPTGAAGRDGRLRVGLRLLEVNQQSLLGLTHGEAVQLLRSVGDTLTVLVCDGFEASTDAALEVSPGVIANPFAAGIGHRNSLESISSIDRELSPEGPGKEKELPGQTLHWGPEATEAAGRGLQPLKLDYRALAAVPSAGSVQRVPSGAAGGKMAESPCSPSGQQPPSPPSPDELPANVKQAYRAFAAVPTSHPPEDAPAQPPTPGPAASPEQLSFRERQKYFELEVRVPQAEGPPKRVSLVGADDLRKMQEEEARKLQQKRAQMLREAAEAGAEARLALDGETLGEEEQEDEQPPWASPSPTSRQSPASPPPLGGGAPVRTAKAERRHQERLRVQSPEPPAPERALSPAELRALEAEKRALWRAARMKSLEQDALRAQMVLSRSQEGRGTRGPLERLAEAPSPAPTPSPTPVEDLGPQTSTSPGRLSPDFAEELRSLEPSPSPGPQEEDGEVALVLLGRPSPGAVGPEDVALCSSRRPVRPGRRGLGPVPS tshr_human Thyrotropin receptor TSHR LGR3 Thyroid-stimulating hormone receptor protein protein human Homo sapiens Human MRPADLLQLVLLLDLPRDLGGMGCSSPPCECHQEEDFRVTCKDIQRIPSLPPSTQTLKLIETHLRTIPSHAFSNLPNISRIYVSIDVTLQQLESHSFYNLSKVTHIEIRNTRNLTYIDPDALKELPLLKFLGIFNTGLKMFPDLTKVYSTDIFFILEITDNPYMTSIPVNAFQGLCNETLTLKLYNNGFTSVQGYAFNGTKLDAVYLNKNKYLTVIDKDAFGGVYSGPSLLDVSQTSVTALPSKGLEHLKELIARNTWTLKKLPLSLSFLHLTRADLSYPSHCCAFKNQKKIRGILESLMCNESSMQSLRQRKSVNALNSPLHQEYEENLGDSIVGYKEKSKFQDTHNNAHYYVFFEEQEDEIIGFGQELKNPQEETLQAFDSHYDYTICGDSEDMVCTPKSDEFNPCEDIMGYKFLRIVVWFVSLLALLGNVFVLLILLTSHYKLNVPRFLMCNLAFADFCMGMYLLLIASVDLYTHSEYYNHAIDWQTGPGCNTAGFFTVFASELSVYTLTVITLERWYAITFAMRLDRKIRLRHACAIMVGGWVCCFLLALLPLVGISSYAKVSICLPMDTETPLALAYIVFVLTLNIVAFVIVCCCYVKIYITVRNPQYNPGDKDTKIAKRMAVLIFTDFICMAPISFYALSAILNKPLITVSNSKILLVLFYPLNSCANPFLYAIFTKAFQRDVFILLSKFGICKRQAQAYRGQRVPPKNSTDIQVQKVTHDMRQGLHNMEDVYELIENSHLTPKKQGQISEEYMQTVL d3zws0_rat Scrib RGD1565055_predicted rCG_60094 protein protein rat Rattus norvegicus (Rat) Rat MLKCIPLWRCNRHVESVDKRHCSLQVVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDVSRNDIPEIPESIKFCKALEIADFSGNPLSRLPDGFTQLRSLAHLALNDVSLQALPGDVGNLANLVTLELRENLLKSLPASLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENRLEELPVELGGLALLTDLLLSQNLLQRLPEGIGQLKQLSILKVDQNRLCEVTEAIGDCENLSELILTENLLTALPRSLGKLTKLTNLNVDRNHLEVLPPEIGGCVALSVLSLRDNRLAVLPPELAHTAELHVLDVAGNRLRSLPFALTHLNLKALWLAENQAQPMLRFQTEDDAQTGEKVLTCYLLPQQPLPSLEDPGQQSSPSESCSDAPLSRVSVIQFEDPLEGEEDAEEAAAEKRGLQRRATPHPSELKVMKRGIEERRNEAFVCKPDPSPPSPSEEERRLSAESALSGNSVPSASTASDGEPETLPAEVQGLGQQEAMPGQEEYPEEDYNEPTVHFAEDTLIPREDGEGEEGQPEAPWPLPSGRQRLIRKDTPHYKKHFKISKLPQPEAVVALLQGVQTDREGPTAGWHNGPHTPWAPRAHEEEEEEENRDEEEGEASMEEDDKEEAVASAPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAAHAGVRVGDKLLEVNGVALQDAEHHEAVEALRGAGAAVQMRVWRERMVEPENAVTITPLRPEDDYSPREWRGGGLRLPLLQPETPVPLRQRHAACLVRSEKGLGFSIAGGKGSTPYRAGDAGIFISRIAEGGAAHRAGTLQVGDRVLSINGVDMTEARHDHAVSLLTAASTTISLLLERETGGAYPPSPPPHSSSTPTATVAATMTTAIPGEPLLPRLSPSLLATALEGPYPVEEICLPRAGGPLGLSIVGGSDHSSHPFGVQDPGVFISKVLPRGLAARCGLRVGDRILAVNGQDVREATHQEAVSALLRPCLELCLLVRRDPPPPGMRELCIQKAPGEKLGISIRGGAKGHAGNPCDPTDEGIFISKVSPTGAAGRDGRLRVGLRLLEVNQQSLLGLTHAEAVQLLRSVGDTLTVLVCDGFDTSTTTALEVSPGVIANPFAAGLGHRNSLESISSIDRELSPEGPGKEKELASQTLPWEPESAETTGRNQEPLKLDYRVLAAVPSAGSLQRGPCATTGGKMTEAPSSPGSQQTKPGVIQPLAQAWPRDSPAPRGRDGPCSPPSPDELPANVKQAYRAFAAVPTVHPPENSATQPPTPGPAASPEQLSFRERQKYFELEVRVPQAEGPPKRVSLVGADDLRKMQEEEARKLQQKRAQMLREEAVTSGPDTGLASDRESLDDEQEAEQPWAGPSHAGGLSPSSPTPLGGSAPVRTAKAERRHQERLRMQSPELPAPERALSPAERRALEAEKRALWRAARMKSLEQDALRAQMVLSKSQEGRGKRGPLERLAEAPSPAPTPSPTPLEDFGLQTSTSPGRLPLSGKKFDYRAFAALPSSRPVYDIQSPDFVEELRTLDPSPSPGPQEEDGEVALVLLGRPSPGTVGPEDMTLCSSRRAMRPGRRGLGPVPS tshr_rat Thyrotropin receptor Tshr Thyroid-stimulating hormone receptor protein protein rat Rattus norvegicus (Rat) Rat MRPGSLLQLTLLLALPRSLWGRGCTSPPCECHQEDDFRVTCKELHQIPSLPPSTQTLKLIETHLKTIPSLAFSSLPNISRIYLSIDATLQRLEPHSFYNLSKMTHIEIRNTRSLTYIDPDALTELPLLKFLGIFNTGLRIFPDLTKIYSTDVFFILEITDNPYMTSVPENAFQGLCNETLTLKLYNNGFTSIQGHAFNGTKLDAVYLNKNKYLTAIDKDAFGGVYSGPTLLDVSSTSVTALPSKGLEHLKELIAKNTWTLKKLPLSLSFLHLTRADLSYPSHCCAFKNQKKIRGILESLMCNESSIRNLRQRKSVNVMRGPVYQEYEEGLGDNHVGYKQNSKFQEGPSNSHYYVFFEEQEDEIIGFGQELKNPQEETLQAFDSHYDYTVCGDNEDMVCTPKSDEFNPCEDIMGYKFLRIVVWFVSPMALLGNVFVLFVLLTSHYKLTVPRFLMCNLAFADFCMGVYLLLIASVDLYTHTEYYNHAIDWQTGPGCNTAGFFTVFASELSVYTLTVITLERWYAITFAMRLDRKIRLRHAYTIMAGGWVSCFLLALLPMVGISSYAKVSICLPMDTDTPLALAYIALVLLLNVVAFVIVCSCYVKIYITVRNPQYNPRDKDTKIAKRMAVLIFTDFMCMAPISFYALSALMNKPLITVTNSGVLLVLFYPLNSCANPFLYAIFTKAFQRDVFILLSKFGLCKHQAQAYQAQRVCPNNNTGIQIQKIPQDTRQSLPNVQDTYEPLGSSHLTPKLQGRISEEYTQTAL f1pky2_canlf SCRIB protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MQLVELDVSRNDIPEIPESIKFCKALEIADFSGNPLSRLPEGFTQLRSLAHLALNDVSLQALPGDVGNLANLVTLELRENLLKSLPSSLSFLVKLEQLDLGGNELEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENRLEELPAELGGLLLLTDLLLSQNLLQRLPDGIGQLKQLSILKVDQNRLCEVTEAIGDCENLSELILTENLLTALPRSLGKLTKLTNLNADRNRLEVLPPEIGGCAALSVLSLRDNRLATLPAELAHTAELHVLDVAGNRLQSLPFALTHLNLKALWLAENQAQPMLRFQTEDDAQTGEKVLTCYLLPQQPPPSLEESGLQSSPSESWSDAPPSRVSVIQFLEVPMCSDDAEGAAAEKRGLQRRATPHPSELKVMKRGVEERRGEAYSWKPESRLPSPLEEDKRLSTESGLSEDSQPSTGTASQGEPEGSLADTQGLSQQEAAPNAQEEAVEEETYEEPTVRFAEDTLLLPPAGEDGESEEGQLEAPWPLPGGRQRLIRKDTPHYKKHFKISKLPQPEAVVALLQGAQPDGEGPAGAGGWHNGPHTPWAPRAEEEDEEDEEEDEEEEEVAVAEEDKEEAVVSAPSIKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAAQAGVRVGDKLLEVNGVALHGAEHHQAVEALRGAGTTVQMRLWRERMVEPENAVTVTPLRPEDDYSPRERRGAGLRLPLLQPEAAGPLRQRHVACLVRSEKGLGFSIAGGKGSTPYRAGDGGIFISRIAEGGAAHRAGTLQVGDRVLSINGVDMTEARHDHAVSLLTAASPTIALLLEREAGGPLPPSPLPHSPPPPVTAPSTVVTASPGESGPLRLAPSLLAATLEGPYPVEEICLPRAGGPLGLSIVGGSDHSSHPFGIQEPGVFISKVLPRGLAARSGLRVGDRILAVNGQDIREATHQEAVSALLRPCLELVLLVRRDPPPPGMRELCIQKAPGEKLGISVRGGAKGHAGNPCDPTDEGIFISKVSPTGAAGRDGRLRVGLRLLEVNQQSLLGLTHGEAVQLLRSVGDTLTVLVCDGFDTSTVAPAEVSPGVIANPFAAGVGRRNSLESVSSIDRELSPEGCGKEKEPPGPTPQWGLEAMVPPGTTGGKMAEGPHSSSCQQPPSPPSPDTLPTNVKQAYRTFAAVPGPHPLQDTPAQPPTPGPMASPEQLSFRERQKYFELEVRMPQAEGPPKRVSLVGADDLRKMQEEEARKLQQKRAQLMREAEDGALSLDLDGEAPDDEEPEEPPPWAGPAAGLSPSSPQPLGGGAPVRTAKAERRHQERLRVQSPELSTPLPDRALSPAERRALEAEKRALWRAARMKSLEQDALRAQMVLSKSQEGRSRRGPLERLAEAPSPAPTPSPTPVEDLGLQTSTSPGRLALSGRKFDYRVFAALPSSRPVCELQSPDFAEELRSLEPSPSPGLQEEDGEVAMVLLGRPSPGTVGPEEVTLCSSRRPVRPGRRGLGPVPS tshr-scrib-1 2960209 N/A 2960215 unspecified role unspecified role prey prey pdz domain 1 sufficient to bind sufficient binding region sufficient to bind range range range range false pdz domain 3 sufficient to bind sufficient binding region sufficient to bind range range range range false N/A 2960216 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2c and d tshr-scrib-2 2960210 N/A 2960216 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 2960215 unspecified role unspecified role prey prey cell lysate cell lysate human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association false false false 2e tshr-scrib-5 2960211 N/A 2960216 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 2960215 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection physical association physical association false false false 3A TSH tshr-scrib-6 2960212 N/A 2960217 unspecified role unspecified role prey prey N/A 2960218 unspecified role unspecified role bait bait physical association physical association false false false 3C tshr-scrib-7 2960213 N/A 2960217 unspecified role unspecified role neutral component neutral component N/A 2960218 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4B scrib-tshr-1 2960214 N/A 2960219 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection N/A 2960216 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection colocalization colocalization false false false 4A