DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ siwanowicz-2005-1 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Siwanowicz I., Popowicz GM., Wisniewska M., Huber R., Kuenkele KP., Lang K., Engh RA., Holak TA. DIP original experiment: DIP-132735X Structure (London, England : 1993) 2005 IMEx Structural basis for the regulation of insulin-like growth factors by IGF binding proteins. Siwanowicz I., Popowicz GM., Wisniewska M., Huber R., Kuenkele KP., Lang K., Engh RA., Holak TA. Only protein-protein interactions imex curation siwanowicz-2005-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Siwanowicz I., Popowicz GM., Wisniewska M., Huber R., Kuenkele KP., Lang K., Engh RA., Holak TA. DIP original experiment: DIP-132737X Structure (London, England : 1993) 2005 IMEx Structural basis for the regulation of insulin-like growth factors by IGF binding proteins. Siwanowicz I., Popowicz GM., Wisniewska M., Huber R., Kuenkele KP., Lang K., Engh RA., Holak TA. Only protein-protein interactions imex curation siwanowicz-2005-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Siwanowicz I., Popowicz GM., Wisniewska M., Huber R., Kuenkele KP., Lang K., Engh RA., Holak TA. DIP original experiment: DIP-132733X Structure (London, England : 1993) 2005 IMEx Structural basis for the regulation of insulin-like growth factors by IGF binding proteins. Siwanowicz I., Popowicz GM., Wisniewska M., Huber R., Kuenkele KP., Lang K., Engh RA., Holak TA. Only protein-protein interactions imex curation siwanowicz-2005-4 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Siwanowicz I., Popowicz GM., Wisniewska M., Huber R., Kuenkele KP., Lang K., Engh RA., Holak TA. DIP original experiment: DIP-132736X Structure (London, England : 1993) 2005 IMEx Structural basis for the regulation of insulin-like growth factors by IGF binding proteins. Siwanowicz I., Popowicz GM., Wisniewska M., Huber R., Kuenkele KP., Lang K., Engh RA., Holak TA. Only protein-protein interactions imex curation siwanowicz-2005-5 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Siwanowicz I., Popowicz GM., Wisniewska M., Huber R., Kuenkele KP., Lang K., Engh RA., Holak TA. DIP original experiment: DIP-132734X Structure (London, England : 1993) 2005 IMEx Structural basis for the regulation of insulin-like growth factors by IGF binding proteins. Siwanowicz I., Popowicz GM., Wisniewska M., Huber R., Kuenkele KP., Lang K., Engh RA., Holak TA. Only protein-protein interactions imex curation igf1_human Insulin-like growth factor I IGF1 IBP1 Somatomedin-C Mechano growth factor protein protein human Homo sapiens Human MGKISSLPTQLFKCCFCDFLKVKMHTMSSSHLFYLALCLLTFTSSATAGPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSARSVRAQRHTDMPKTQKYQPPSTNKNTKSQRRKGWPKTHPGGEQKEGTEASLQIRGKKKEQRREIGSRNAECRGKKGK The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation mint ibp4_human Insulin-like growth factor-binding protein 4 IGFBP4 IBP4 protein protein human Homo sapiens Human MLPLCLVAALLLAAGPGPSLGDEAIHCPPCSEEKLARCRPPVGCEELVREPGCGCCATCALGLGMPCGVYTPRCGSGLRCYPPRGVEKPLHTLMHGQGVCMELAEIEAIQESLQPSDKDEGDHPNNSFSPCSAHDRRCLQKHFAKIRDRSTSGGKMKVNGAPREDARPVPQGSCQSELHRALERLAASQSRTHEDLYIIPIPNCDRNGNFHPKQCHPALDGQRGKCWCVDRKTGVKLPGGLEPKGELDCHQLADSFRE ibp5_human Insulin-like growth factor-binding protein 5 IGFBP5 IBP5 protein protein human Homo sapiens Human MVLLTAVLLLLAAYAGPAQSLGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRIISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE igf1-igfbp4-1 3137598 N/A 3137603 unspecified role unspecified role unspecified role unspecified role Mature protein Region 49-118 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3137604 unspecified role unspecified role unspecified role unspecified role N-terminal Domain Region 24-103 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false The range was changed because the region in the paper corresponded to the N-terminal of the mature protein, ecolx Escherichia coli direct interaction direct interaction false false false igf1-igfbp4-2 3137599 N/A 3137603 unspecified role unspecified role unspecified role unspecified role Mature protein Region 49-118 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3137604 unspecified role unspecified role unspecified role unspecified role N-terminal Domain Region 24-103 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false The range was changed because the region in the paper corresponded to the N-terminal of the mature protein, C-terminal domain Region 172-253 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false The range was changed because the region in the paper corresponded to the C-terminal of the mature protein, ecolx Escherichia coli direct interaction direct interaction false false false igf1-igfbp4-3 3137600 N/A 3137603 unspecified role unspecified role unspecified role unspecified role Mature protein Region 49-118 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3137604 unspecified role unspecified role unspecified role unspecified role N-terminal Domain Region 24-103 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false The range was changed because the region in the paper corresponded to the N-terminal of the mature protein, ecolx Escherichia coli direct interaction direct interaction false false false igf1-igfbp5-1 3137601 N/A 3137603 unspecified role unspecified role unspecified role unspecified role Mature protein Region 49-118 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3137605 unspecified role unspecified role unspecified role unspecified role N-terminal Domain Region 60-103 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false The range was changed because the region in the paper corresponded to the N-terminal of the mature protein, P24593:p.Leu94Met L94M mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false igf1-igfbp4-4 3137602 N/A 3137603 unspecified role unspecified role unspecified role unspecified role Mature protein Region 49-118 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3137604 unspecified role unspecified role unspecified role unspecified role N-terminal Domain Region 24-103 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false The range was changed because the region in the paper corresponded to the N-terminal of the mature protein, ecolx Escherichia coli direct interaction direct interaction false false false