UniProt UniProt http://www.uniprot.org wang-2005-4 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Wang D., De Deken X., Milenkovic M., Song Y., Pirson I., Dumont JE., Miot F. CoIP experiments with human thyrocytes using anti-Duox for immunoprecipitation omitted as the antibody recognizes DUOX1 and DUOX2 proteins (here as baits, fig 6D) dawang@ulb.ac.be The Journal of biological chemistry 2005 imex curation Only protein-protein interactions wang-2005-6 chlae-cos Chlorocebus aethiops cos cos anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Wang D., De Deken X., Milenkovic M., Song Y., Pirson I., Dumont JE., Miot F. CoIP experiments with human thyrocytes using anti-Duox for immunoprecipitation omitted as the antibody recognizes DUOX1 and DUOX2 proteins (here as baits, fig 6D) dawang@ulb.ac.be The Journal of biological chemistry 2005 imex curation Only protein-protein interactions wang-2005-3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Wang D., De Deken X., Milenkovic M., Song Y., Pirson I., Dumont JE., Miot F. CoIP experiments with human thyrocytes using anti-Duox for immunoprecipitation omitted as the antibody recognizes DUOX1 and DUOX2 proteins (here as baits, fig 6D) dawang@ulb.ac.be dog thyroid cDNA The Journal of biological chemistry 2005 imex curation Only protein-protein interactions wang-2005-5 chlae-cos Chlorocebus aethiops cos cos confocal microscopy confocal microscopy immunostaining immunostaining Wang D., De Deken X., Milenkovic M., Song Y., Pirson I., Dumont JE., Miot F. CoIP experiments with human thyrocytes using anti-Duox for immunoprecipitation omitted as the antibody recognizes DUOX1 and DUOX2 proteins (here as baits, fig 6D) dawang@ulb.ac.be The Journal of biological chemistry 2005 imex curation Only protein-protein interactions f1pld0_canlf TXNDC11 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MRAFLGLTIICPHVLCAEHSSSRAKDVIIPAKPPVSFFSSRSPVLDLFQGQLDYAEHVRRDSEVVLLFFYAPWCGQSIAARSEIEQAASQLSDQVLFVAINCWWNQGKCRKQKHFFYFPVIYLYHRSFGPIEYKGPMSAVYIEKFVRRVMKPLLYIPSQSELLDFLSNYEPGVLGYFEFSGSPQPPGYLTFFTSALHSLKKDYLGTVRFGVITNKHLAKLVSLVHSGSVYLHRHFNTSLVFPREAVNYTAENIHKWALENREALLRWLRPHGGKSLLLNNELKKGPALLVFLPFNPLAESHPLIDEITEVALEYNNCHGDQVVEQLLQHLRRADAPLLRPLAPEPPEQLPEPPLITASPCCNTVVLPQWHSVSRTHNVCELCVNQSTGGVRPSSVGMPQCSFFEMAAALDSFYLKEQTFYHVASDSIECSNFLSSYSPFSYYTACCRTINRAVTGFIDSGQSVFETPTITFSSLEKKCEVDPPGSVPHIEENRYLFPEVDVSSTNFTGLSCRTNKTLNIYLLDSNLFWLYAERLGAPSATRVKEFATIVDVKEESHYILDPKQALMKFTLESFIQNFSVLYSPLKRHLIGSDSAQFPSQHLITEVTTDTFWEVVLQKQDVLLLYYAQWCGFCPSLNHVFIQLARLLPSDTFTVARIDVSQNDLPWEFMVDRLPTVLFFPCNRKDRSVKYPEDLPVTLPNLLRFILHHSDPASTPQNLANPPTKECLQSEAVFQQGHISHLEREIQKLRAEISTLQRAQVQVEAQLSSARRDEHRLLRQKQTLEKQHSLLQLHSEQLQTLYEQKTHELEEVAQKLQELADASENLLTENTWLKILVATMERKLEGKDGAENLTPHKEAGSGHPNPSGVPRLPGGSPPPSNSSSTLASERSNENRTD duox1_canlf Dual oxidase 1 DUOX1 THOX1 NADPH thyroid oxidase 1 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MGFCLALTWTFLVGSWTSMGAQKPISWEVQRFDGWYNNLMEHKWGSKGSRLQRLVPASYADGVYQPLGEPHLPNPRDLSNAAMRGPAGQASLRNRTVLGVFFGYHVLSDLVSVETPGCPAEFLNIRIPPGDPVFDPNGRGDVVLPFQRSRWDPESGQSPSNPRDLTNAVTGWLDGSAIYGSSHSWSDALRSFSGGQLASGPDPAFPRNAQPPLLMWSAPDPASGQRGPGGLYAFGAERGNRDPFLQALGLLWFRYHNLCAQRLARQHPHWGDEELFQHARKRVIATYQNIALYEWLPSFLQQAPVKYAGYNPFLDPSISPEFLVASEQFFSTMVPPGIYMRNASCHFQEVINRNSSISRALRVCNSYWSRKHPNLRRAEDVDALLLGMASQIAEREDHVVVEDVLDFWPGSLKFSRTDHVAGCLQRGRDLGLPSYTKARAALGLPPITRWQDINPALSQNNHTVLEATAALYNQDLSQLELLPGGLLESHGDPGPLFSAIVLNQFVRLRDGDRYWFENTRNGLFSEEEIAEIRNTSLRDVLVAVTNMNPSTLQPNVFFWHMGDPCPQPRQLSTQGLPACAPSTMQDYFEGSGFGFGVTIGTLCCFPLVSLLSAWIVARLRKKNFKKLQGQDRKSVMSEKLVGGMEALEWQGHKEPCRPVLVHLQPGQICVVDGRLSVLRTIQLRPPQQVNLILSGNRGRRALLLKIPKEYDLVLLFNLEEERQVLVENLRGALKESGLKFQEWELREQELMRTAVTRQQRSHLLETFFRHLFSQVLDIDQADAGTLPLDSSQKVQEALTCELSRAEFAESLGLKPQDMFVESMFSLADKDGNGYLSFREFLDILVVFMKGSPEEKSRLMFRMYDFDGNGLISKDEFIRMLRSFIEISNNCLSKAQLTEVVESMFRESGFQDKEELTWEDFHFMLRDHDSELRFTQLCVRGVEVPEVIKDLCRRASYISQEKICPSPRVSARCPHSNTEVEWTPQRLQCPVDTDPPQEIRRRFGKKVTSFQPLLFTEAQREKFQRSRRHQTLQQFKRFIENYRRHIGCVAVFYAITGGLFLERAYYYAFGAHHMGITDTTRVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFLNRYVPFDAAVDFHRLIASTAIVLTVLHSAGHVVNVYLFSISPLSVLSCLFPGLFHNDGSEFPQKYYWWFFQTVPGLTGVMLLLVLAIMYVFASHHFRRHSFRGFWLTHHLYILLYVLLIIHGSFGLIQLPRFHIFFLVPALIYVGDKLVSLSRKKVEISVVKAELLPSGVTHLQFQRPQGFEYKSGQWVQIACLALGTTEYHPFTLTSAPHEDTLSLHIRAAGPWTTRLREIYSPPTGDGCAKYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSVSCQVFCKKIYFIWVTRTQRQFEWLADIIREVEENDCQDLVSVHIYITQLAEKFDLRTTMLYICERHFQKVLNRSLFTGLRSITHFGRPPFEPFFKSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLINRQDRTHFSHHYENF duox1_human Dual oxidase 1 DUOX1 DUOX LNOX1 THOX1 Large NOX 1 Long NOX 1 NADPH thyroid oxidase 1 Thyroid oxidase 1 protein protein human Homo sapiens Human MGFCLALAWTLLVGAWTPLGAQNPISWEVQRFDGWYNNLMEHRWGSKGSRLQRLVPASYADGVYQPLGEPHLPNPRDLSNTISRGPAGLASLRNRTVLGVFFGYHVLSDLVSVETPGCPAEFLNIRIPPGDPMFDPDQRGDVVLPFQRSRWDPETGRSPSNPRDPANQVTGWLDGSAIYGSSHSWSDALRSFSRGQLASGPDPAFPRDSQNPLLMWAAPDPATGQNGPRGLYAFGAERGNREPFLQALGLLWFRYHNLWAQRLARQHPDWEDEELFQHARKRVIATYQNIAVYEWLPSFLQKTLPEYTGYRPFLDPSISSEFVAASEQFLSTMVPPGVYMRNASCHFQGVINRNSSVSRALRVCNSYWSREHPSLQSAEDVDALLLGMASQIAEREDHVLVEDVRDFWPGPLKFSRTDHLASCLQRGRDLGLPSYTKARAALGLSPITRWQDINPALSRSNDTVLEATAALYNQDLSWLELLPGGLLESHRDPGPLFSTIVLEQFVRLRDGDRYWFENTRNGLFSKKEIEEIRNTTLQDVLVAVINIDPSALQPNVFVWHKGDPCPQPRQLSTEGLPACAPSVVRDYFEGSGFGFGVTIGTLCCFPLVSLLSAWIVARLRMRNFKRLQGQDRQSIVSEKLVGGMEALEWQGHKEPCRPVLVYLQPGQIRVVDGRLTVLRTIQLQPPQKVNFVLSSNRGRRTLLLKIPKEYDLVLLFNLEEERQALVENLRGALKESGLSIQEWELREQELMRAAVTREQRRHLLETFFRHLFSQVLDINQADAGTLPLDSSQKVREALTCELSRAEFAESLGLKPQDMFVESMFSLADKDGNGYLSFREFLDILVVFMKGSPEEKSRLMFRMYDFDGNGLISKDEFIRMLRSFIEISNNCLSKAQLAEVVESMFRESGFQDKEELTWEDFHFMLRDHNSELRFTQLCVKGVEVPEVIKDLCRRASYISQDMICPSPRVSARCSRSDIETELTPQRLQCPMDTDPPQEIRRRFGKKVTSFQPLLFTEAHREKFQRSCLHQTVQQFKRFIENYRRHIGCVAVFYAIAGGLFLERAYYYAFAAHHTGITDTTRVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFLNRYVPFDAAVDFHRLIASTAIVLTVLHSVGHVVNVYLFSISPLSVLSCLFPGLFHDDGSELPQKYYWWFFQTVPGLTGVVLLLILAIMYVFASHHFRRRSFRGFWLTHHLYILLYVLLIIHGSFALIQLPRFHIFFLVPAIIYGGDKLVSLSRKKVEISVVKAELLPSGVTHLRFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAPHEDTLSLHIRAAGPWTTRLREIYSAPTGDRCARYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSVSCQVFCKKIYFIWVTRTQRQFEWLADIIREVEENDHQDLVSVHIYITQLAEKFDLRTTMLYICERHFQKVLNRSLFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLINRQDRTHFSHHYENF txd11_human Thioredoxin domain-containing protein 11 EF-hand-binding protein 1 TXNDC11 EFP1 protein protein human Homo sapiens Human MSECGGRGGGSSSSEDAEDEGGGGGGPAGSDCLSSSPTLATASSAGRLRRGLRGAFLMARQRPELLCGAVALGCALLLALKFTCSRAKDVIIPAKPPVSFFSLRSPVLDLFQGQLDYAEYVRRDSEVVLLFFYAPWCGQSIAARAEIEQAASRLSDQVLFVAINCWWNQGKCRKQKHFFYFPVIYLYHRSFGPIEYKGPMSAVYIEKFVRRVMKPLLYIPSQSELLDFLSNYEPGVLGYFEFSGSPQPPGYLTFFTSALHSLKKALESTSSPRALVSFTGEWHLETKIYVLDYLGTVRFGVITNKHLAKLVSLVHSGSVYLHRHFNTSLVFPREVLNYTAENICKWALENQETLFRWLRPHGGKSLLLNNELKKGPALFLFIPFNPLAESHPLIDEITEVALEYNNCHGDQVVERLLQHLRRVDAPVLESLALEVPAQLPDPPTITASPCCNTVVLPQWHSFSRTHNVCELCVNQTSGGMKPSSVSVPQCSFFEMAAALDSFYLKEQTFYHVASDSIECSNFLTSYSPFSYYTACCRTISRGVSGFIDSEQGVFEAPTVAFSSLEKKCEVDAPSSVPHIEENRYLFPEVDMTSTNFTGLSCRTNKTLNIYLLDSNLFWLYAERLGAPSSTQVKEFAAIVDVKEESHYILDPKQALMKLTLESFIQNFSVLYSPLKRHLIGSGSAQFPSQHLITEVTTDTFWEVVLQKQDVLLLYYAPWCGFCPSLNHIFIQLARNLPMDTFTVARIDVSQNDLPWEFMVDRLPTVLFFPCNRKDLSVKYPEDVPITLPNLLRFILHHSDPASSPQNVANSPTKECLQSEAVLQRGHISHLEREIQKLRAEISSLQRAQVQVESQLSSARRDEHRLRQQQRALEEQHSLLHAHSEQLQALYEQKTRELQELARKLQELADASENLLTENTWLKILVATMERKLEGRDGAESLAAQREVHPKQPEPSATPQLPGSSPPPANVSATLVSERNKENRTD duox2_human Dual oxidase 2 DUOX2 LNOX2 THOX2 Large NOX 2 Long NOX 2 NADH/NADPH thyroid oxidase p138-tox NADPH oxidase/peroxidase DUOX2 NADPH thyroid oxidase 2 Thyroid oxidase 2 p138 thyroid oxidase protein protein human Homo sapiens Human MLRARPEALMLLGALLTGSLGPSGNQDALSLPWEVQRYDGWFNNLRHHERGAVGCRLQRRVPANYADGVYQALEEPQLPNPRRLSNAATRGIAGLPSLHNRTVLGVFFGYHVLSDVVSVETPGCPAEFLNIRIPPGDPVFDPDQRGDVVLPFQRSRWDPETGRSPSNPRDLANQVTGWLDGSAIYGSSHSWSDALRSFSGGQLASGPDPAFPRDSQNPLLMWAAPDPATGQNGPRGLYAFGAERGNREPFLQALGLLWFRYHNLWAQRLARQHPDWEDEELFQHARKRVIATYQNIAVYEWLPSFLQKTLPEYTGYRPFLDPSISPEFVVASEQFFSTMVPPGVYMRNASCHFRKVLNKGFQSSQALRVCNNYWIRENPNLNSTQEVNELLLGMASQISELEDNIVVEDLRDYWPGPGKFSRTDYVASSIQRGRDMGLPSYSQALLAFGLDIPRNWSDLNPNVDPQVLEATAALYNQDLSQLELLLGGLLESHGDPGPLFSAIVLDQFVRLRDGDRYWFENTRNGLFSKKEIEDIRNTTLRDVLVAVINIDPSALQPNVFVWHKGAPCPQPKQLTTDGLPQCAPLTVLDFFEGSSPGFAITIIALCCLPLVSLLLSGVVAYFRGREHKKLQKKLKESVKKEAAKDGVPAMEWPGPKERSSPIIIQLLSDRCLQVLNRHLTVLRVVQLQPLQQVNLILSNNRGCRTLLLKIPKEYDLVLLFSSEEERGAFVQQLWDFCVRWALGLHVAEMSEKELFRKAVTKQQRERILEIFFRHLFAQVLDINQADAGTLPLDSSQKVREALTCELSRAEFAESLGLKPQDMFVESMFSLADKDGNGYLSFREFLDILVVFMKGSPEDKSRLMFTMYDLDENGFLSKDEFFTMMRSFIEISNNCLSKAQLAEVVESMFRESGFQDKEELTWEDFHFMLRDHDSELRFTQLCVKGGGGGGNGIRDIFKQNISCRVSFITRTPGERSHPQGLGPPAPEAPELGGPGLKKRFGKKAAVPTPRLYTEALQEKMQRGFLAQKLQQYKRFVENYRRHIVCVAIFSAICVGVFADRAYYYGFASPPSDIAQTTLVGIILSRGTAASVSFMFSYILLTMCRNLITFLRETFLNRYVPFDAAVDFHRWIAMAAVVLAILHSAGHAVNVYIFSVSPLSLLACIFPNVFVNDGSKLPQKFYWWFFQTVPGMTGVLLLLVLAIMYVFASHHFRRRSFRGFWLTHHLYILLYALLIIHGSYALIQLPTFHIYFLVPAIIYGGDKLVSLSRKKVEISVVKAELLPSGVTYLQFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAPHEDTLSLHIRAVGPWTTRLREIYSSPKGNGCAGYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSLGSQMLCKKIYFIWVTRTQRQFEWLADIIQEVEENDHQDLVSVHIYVTQLAEKFDLRTTMLYICERHFQKVLNRSLFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLVNRQDRAHFMHHYENF duox1-txndc11-2 2872022 N/A 2872026 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 2872027 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 2872035 2872033 physical association physical association false false false 1 duox1-txndc11-3 2872022 N/A 2872026 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 2872028 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 2872040 2872038 physical association physical association false false false 1 duox1-txndc11-5 2872023 N/A 2872029 anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2872028 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection physical association physical association false false false 6B abd C Presence of EGTA or Ca(2+) or both does not affect association duox2-txndc11-1 2872023 N/A 2872030 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection N/A 2872029 anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 6B duox1-txndc11-1 2872024 N/A 2872026 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 2872027 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 2872053 2872051 physical association physical association false false false no figure duox1-txndc11-4 2872025 N/A 2872029 anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2872028 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection colocalization colocalization false false false 5