UniProt UniProt http://www.uniprot.org rho-2000-2 yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Rho SB., Martinis SA. smartinis@uh.edu RNA (New York, N.Y.) 2000 imex curation Only protein-protein interactions imex curation rho-2000-1 yeast Saccharomyces cerevisiae Baker's yeast rna three hybrid rna three hybrid Three hybrid system rna 3-hybrid rna tri hybrid rna-three hybrid predetermined predetermined participant predetermined Rho SB., Martinis SA. The DNA binding domain -LexADB was fused to an RNA binding protein -MS2-coat protein rather than Hiv-1 RevM10. A third component is hybrid RNA comprised of MS2 RNA fused to the RNA of interest (Bait). The protein prey fused to B42-AD. smartinis@uh.edu RNA (New York, N.Y.) 2000 imex curation Only protein-protein interactions imex curation rho-2000-3 yeast Saccharomyces cerevisiae Baker's yeast 3 hybrid three hybrid 3-hybrid tri hybrid tri-hybrid assay 3 hybrid predetermined predetermined participant predetermined Rho SB., Martinis SA. The DNA binding domain -LexADB was fused to an RNA binding protein -MS2-coat protein rather than Hiv-1 RevM10. A third component is hybrid RNA comprised of MS2 RNA fused to the RNA of interest (Bait). The protein prey fused to B42-AD. smartinis@uh.edu RNA (New York, N.Y.) 2000 imex curation Only protein-protein interactions imex curation mbi4_yeast Intron-encoded RNA maturase bI4 RNA maturase SCbI4 BI4 SCBI4 Q0120 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAFRKSNVYLSLVNSYIIDSPQPSSINYWWNMGSLLGLCLVIQIVTGIFMAMHYSSNIELAFSSVEHIMRDVHNGYILRYLHANGASFFFMVMFMHMAKGLYYGSYRSPRVTLWNVGVIIFILTIATAFLGYCCVYGQMSHWGATVITNLFSAIPFVGNDIVSWLWGGFSVSNPTIQRFFALHYLVPFIIAAMVIMHLMALHIHGSSNPLGITGNLDRIPMHSYFIFKDLVTVFLFMLILALFVFYSPNTLGQNMALLLITYVINILCAVCWKSLFIKYQWKIYNKTTYYFIIQNILNTKQLNNFVLKFNWTKQYNKMNIVSDLFNPNRVKYYYKEDNQQVTNMNSSNTHLTSNKKNLLVDTSETTRTTKNKFNYLLNIFNMKKMNQIITKRHYSIYKDSNIRFNQWLAGLIDGDGYFCITKNKYASCEITVELKDEKMLRQIQDKFGGSVKLRSGVKAIRYRLQNKEGMIKLINAVNGNIRNSKRLVQFNKVCILLNIDFKEPIKLTKDNAWFMGFFDADGTINYYYSGKLKIRPQLTISVTNKYLHDVEYYREVFGGNIYFDKAKNGYFKWSINNKELHNIFYTYNKSCPSKSNKGKRLFLIDKFYYLYDLLAFKAPHNTALYKAWLKFNEKWNNN bi4_intron_yeast_mrna bI4 intron RNA mrna messenger rna mRNA mrna yeast Saccharomyces cerevisiae Baker's yeast UCAAAAUAUGGCCUUAUUAUUAAUUACAUAUGUAAUUAAUAUUUUAUGUGCUGUAUGCUGGAAAUCUUUAUUUAUUAAAUAUCAAUGAAAAAUUUAUAAUAAAACUCUAUAUUAUUUUAUUAUUCAAAAUAUUUUAAAUACAAAACAAUUAAAUAAUUUCGUAUUAAAAUUUAAUUGAACAAAGCAAUAUAAUAAAAUAAAUAUUGUAAGUGAUUUAUUUAAUCCCAAUAGAGUAAAAUAUUAUUAUAAAGAAGAUAAUCAGCAGGUAACCAAUAUAAAUUCUUCUAAUACUCACUUAACGAGUAAUAAAAAGAAUUUAUUAGUAGAUACUUCAGAGACUACACGCACACUAAAAAAUAAAUUUAAUUAUUUAUUAAAUAUUUUUAAUAUAAAAAAAAUAAAUCAAAUUAUUCUUAAAAGACAUUAUAGUAUUUAUAAAGAUAGUAAUAUUAGAUUUAACCAAUGAUUGGCCGGUUUAAUUGACGGAGAUGGUUAUUUUUGUAUUACUAAAAAUAAAUAUGCAUCUUGUGAAAUUCUUGUAGAAUUAAAAGAUGAAAAAAUGUUAAGACAAAUCCAAGAUAAAUUUGGUGGUUCUGUAAAAUUAAGAUCAGGUGUUAAGGCUAUUAGAUAUAGAUUACAAAAUAAAGAAGGUAUAAUUAAAUUAAUUAAUGCCGUUAAUGGUAAUAUUCGUAAUAGUAAAAGAUUAGUACAAUUUAAUAAAGUAUGUAUUUUAUUAAAUAUCGAUUUUAAAGAACCUAUUAAAUUAACUAAAGAUAAUGCUUGAUUUAUAGGGUUCUUUGAUGCUGAUGGUACUAUUAAUUAUUAUUAUUCCGGUAAAUUAAAAAUUAGACCUCAAUUAACUAUUAGCGUUACAAAUAAAUAUUUACAUGAUGUUGAAUACUAUAGAGAAGUAUUUGGUGGUAAUAUUUAUUUUGAUAAAGCUAAAAAUGGUUAUUUUAAAUGAUCUAUUAAUAAUAAAGAAUUACAUAAUAUUUUUUAUCUUUAUAAUAAAAGUUGUCCUUCUAAAUCUAAUAAAGGUAAACGUUUAUUUUUAAUUGAUAAAUUUUAUUAUUUAUAUGAUUUAUUAGCUUUUAAAGCACCUCAUAAUACUGCUUUAUAUAAAGCUUGAUUAAAAUUUAAUGAAAAAUGAAAUAAUAAUUAAAUUUUCUCCGUAUUCAUUAUUAUAUUAUCUAAUUUAUAAAAUAUUUAAAGAUUCCUUAUAAUAAUAUAACAUCUUUGUAAAUUAUUGUUAAAGAUAAUAUAAAUUAUUAUGAAUCGGUAGAUUAUAUUUUUACAAUCUUAUUAAAUAAAAUUCUGAUCAUUAAACAUGAUUGAAGAAAUAAUAAUAGUUUAUGAAAUAAGAUAGUGUAAUAUAAAUUUUUAUGAAGAUAUAGUCCAUUUUAUAUUUAUUAUAAAAG sylm_yeast Leucine--tRNA ligase, mitochondrial NAM2 MSL1 YLR382C L3502.6 Leucyl-tRNA synthetase protein protein yeast Saccharomyces cerevisiae Baker's yeast MLSRPSSRFLSTKRGPGPAVKKLIAIGEKWKQKTTRGLPKQDTLNSGSKYILCQFPYPSGALHIGHLRVYVISDSLNRFYKQKGYNVIHPMGWDAFGLPAENAAIERSINPAIWTRDNIAKMKQQMQSMLANFDWDREITTCDPEYYKFTQWIFLKLFENGLAYRKEAEINWDPVDMTVLANEQVDAQGRSWRSGAIVEKKQLKQWFLGITKFAPKLKKHLNQLKDWPSNVKQMQKNWIGESVGAELVFKVADPKFENLIVFTTRPETLFAVQYVALALDHPIVQKYCEEMPDLKEFIQKSDQLPNDTKEGFQLPNIKAVNPLTKEEVPIFAAPYVVSSYGSAPSAVMGCPGHDNRDFEFWQTNCPGEHIKTCIAPFFDDASKVTEQERQRIIDTVPFTSTDGVLTKECGEHSGVLTVVARKSIMGMLNSEGLSKSVVRYKIRDWLISRQRYWGTPIPIIHCDNCGPVPVPESDLPVKLPELEGLDTKGNPLSTIDEFVNVACPSCGSPAKRETDTMDTFIDSSWYYFRFLDPKNTSKPFDREIASKNMPVDIYIGGVEHAILHLLYSRFIAKFLGSINAWSDPAGIFEPFKKLVTQGMVQGKTYVDPDSGKFLKPDELTFVNDSPDGNTVIIKSNGKVPVVSYEKMSKSKYNGADPNECILRHGPDATRAHILFQSPIADALNWDESKIVGIERWLQKVLHLTKNILSLEKDLAISKDYKTPTDLNDAEVKFHNDFQRFLKSITESFEVNLSLNTVISDYMKLTNILESALKKGEVRNEMIVQNLQKLVTVIYPAVPSISEEAAEMINSQMEWNQYRWPEVERTTESKFKKFQIVVNGRVKFMYTADKNFLKLGRDAVIETLMNLPEGRMYLMNKKIKKFVMKFNVISFLFHK syic_human Isoleucine--tRNA ligase, cytoplasmic IARS1 IARS Isoleucyl-tRNA synthetase protein protein human Homo sapiens Human MLQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQCRAIVMRYSAEWKSTVSRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVFVTFPLEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGRLLILMEARLSALYKLESDYEILERFPGAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGAEDYRVCMDFNIIRKDSLPVCPVDASGCFTTEVTDFAGQYVKDADKSIIRTLKEQGRLLVATTFTHSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLRNNDLCYWVPELVREKRFGNWLKDARDWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEELSGAKISDLHRESVDHLTIPSRCGKGSLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLATALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFLYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMTSIKQLSSEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKAKSEGTYLNSVIESHTEFIFTTIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITLTRGSSLPGPACAYVNLNICANGSEQGGVLLLENPKGDNRLDLLKLKSVVTSIFGVKNTELAVFHDETEIQNQTDLLSLSGKTLCVTAGSAPSLINSSSTLLCQYINLQLLNAKPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF syep_human Bifunctional glutamate/proline--tRNA ligase EPRS1 Bifunctional aminoacyl-tRNA synthetase Cell proliferation-inducing gene 32 protein EPRS GLNS Glutamatyl-prolyl-tRNA synthetase PARS QARS QPRS PIG32 protein protein human Homo sapiens Human MATLSLTVNSGDPPLGALLAVEHVKDDVSISVEEGKENILHVSENVIFTDVNSILRYLARVATTAGLYGSNLMEHTEIDHWLEFSATKLSSCDSFTSTINELNHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLKQKKAPVHVKRWFGFLEAQQAFQSVGTKWDVSTTKARVAPEKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYAEKLIQEGKAYVDDTPAEQMKAEREQRIDSKHRKNPIEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPYIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYVALLKKEVIPVNVPEAQEEMKEVAKHPKNPEVGLKPVWYSPKVFIEGADAETFSEGEMVTFINWGNLNITKIHKNADGKIISLDAKLNLENKDYKKTTKVTWLAETTHALPIPVICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCKEAPCVLIYIPDGHTKEMPTSGSKEKTKVEATKNETSAPFKERPTPSLNNNCTTSEDSLVLYNRVAVQGDVVRELKAKKAPKEDVDAAVKQLLSLKAEYKEKTGQEYKPGNPPAEIGQNISSNSSASILESKSLYDEVAAQGEVVRKLKAEKSPKAKINEAVECLLSLKAQYKEKTGKEYIPGQPPLSQSSDSSPTRNSEPAGLETPEAKVLFDKVASQGEVVRKLKTEKAPKDQVDIAVQELLQLKAQYKSLIGVEYKPVSATGAEDKDKKKKEKENKSEKQNKPQKQNDGQRKDPSKNQGGGLSSSGAGEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKDFFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY bi4_intron_yeast_mrna-nam2-2 3315769 N/A 3315772 unspecified role unspecified role bait bait N/A 3315773 unspecified role unspecified role ancillary ancillary N/A 3315774 unspecified role unspecified role prey prey physical association physical association false false false Fig. 5 bi4_intron_yeast_mrna-bi4-2 3315769 N/A 3315774 unspecified role unspecified role bait bait N/A 3315773 unspecified role unspecified role ancillary ancillary N/A 3315772 unspecified role unspecified role prey prey physical association physical association false false false Fig. 5 eprs-iars-1 3315769 N/A 3315775 unspecified role unspecified role bait bait N/A 3315776 unspecified role unspecified role prey prey physical association physical association false false false Fig. 5 bi4_intron_yeast_mrna-nam2-1 3315770 N/A 3315773 unspecified role unspecified role bait bait N/A 3315774 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3 bi4_intron_yeast_mrna-bi4-1 3315770 N/A 3315773 unspecified role unspecified role bait bait N/A 3315772 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3 bi4-nam2-1 3315771 N/A 3315774 unspecified role unspecified role prey prey N/A 3315773 unspecified role unspecified role bait bait N/A 3315772 unspecified role unspecified role bait bait physical association physical association false false false Fig. 7 bi4_intron_yeast_mrna-bi4-6 3315771 N/A 3315774 unspecified role unspecified role bait bait N/A 3315773 unspecified role unspecified role bait bait N/A 3315772 unspecified role unspecified role prey prey physical association physical association false false false Fig. 7