MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint watanabe-2016-1 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Watanabe Y., Kawaguchi K., Okuyama N., Sugawara Y., Obita T., Mizuguchi M., Morita M., Imanaka T. imanaka@pha.u-toyama.ac.jp FEBS letters 2016 Only protein-protein interactions imex curation imex curation watanabe-2016-2 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Watanabe Y., Kawaguchi K., Okuyama N., Sugawara Y., Obita T., Mizuguchi M., Morita M., Imanaka T. imanaka@pha.u-toyama.ac.jp FEBS letters 2016 Only protein-protein interactions imex curation imex curation q57w55_tryb2 Tb927.5.1100 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MDCGAGFALGQQLAKDALHMQGGVRPGTTGNVEQDALMTGMMVPPTGPMEDWAQHFAAHQHHHQQHQQMMMQRQHNDALMIQQQHRDMEEAFRASARAGAPQQANAGPLMMPPGPMMMAGGMAPMMHAGGFMMGGMPQMMPCTPMGMNMGMAPVATMNPATTSTVSGAREGATAVSSAAPGVVDLGGDSAWAEKLHQAEWGQDYKDVEVHTVEGSTAQTVEEHAKTSKFYEFMDKIRKKELLVDEDSGEVVQGPGPDPDVEADTEYLARLAAMEGINVPPSVMDHMQGQDGVQRGTDEDMEGMMGDDVYDPSADVEQWAQEYAQMQAMQERLQNNTDYPFEANNPYMYHENPMEEGLSMLKLANLAEAALAFEAVCQKEPEREEAWRSLGLTQAENEKDGLAIIALNHARMLDPKDIAVHAALAVSHTNEHNANAALASLRAWLLSQPQYEQLGSVNLQADVDIDDLNVQSEDFFFAAPNEYRECRTLLHAALEMNPNDAQLHASLGVLYNLSNNYDSAAANLRRAVELRPDDAQLWNKLGATLANGNRPQEALDAYNRALDINPGYVRVMYNMAVSYSNMSQYDLAAKQLVRAIYMQVGGTTPTGEASREATRSMWDFFRMLLNVMNRPDLVELTYAQNVEPFAKEFGLQSMLL q8iew2_trybb Peroxin-14 PEX14 protein protein trybb Trypanosoma brucei brucei MSLLLSGVVDDGKSKPEVEHTHSEREKRVSNAVEFLLDSRVRRTPTSSKVHFLKSKGLSAEEICEAFTKVGQPKTLNEIKRILSERPYVPTGPNSQHMTQPLRDESADSVPTPHPNQSRRHTSLLYAPQAPPLPEAAAATRGVDWRDLVIGAGAAVIGGFAAFKAFQLYSPYEIRLKDEGSKPRSRRSRRGGRHASSDSEAERSLVHREVPALPVPAIPVASESHVDAKQAEIERLKTELKETQEALEAEKKGKAELSITLGKLRGQVTAYSRTNEKQESQIKSLQEEVNRLKSEIERKEDSAKVNANSNVEETLDSKEEGLPSSDTESSNPNGASSEVARDTQQQDGPAPLSTETDGTVVGGEAA pex14-1 1922983 N/A 1922985 unspecified role unspecified role prey prey purified purified Q57W55:p.[Trp200Ala;Tyr204Ala;Trp318Ala;Tyr322Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q57W55:p.[Trp318Ala;Tyr322Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false undefined mutation mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q57W55:p.[Trp52Ala;Phe56Ala;Trp200Ala;Tyr204Ala;Trp318Ala;Tyr322Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 1922986 unspecified role unspecified role bait bait purified purified gst tag gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false f3 q57w55_tryb2-pex14-2 1922983 N/A 1922985 unspecified role unspecified role prey prey purified purified Q57W55:p.Glu321Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q57W55:p.Glu321His mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1922986 unspecified role unspecified role bait bait purified purified gst tag gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false f5 q57w55_tryb2-pex14-1 1922984 N/A 1922985 unspecified role unspecified role prey prey purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1922986 unspecified role unspecified role bait bait purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1922984 f4 pex14-q57w55_tryb2-1 1922984 N/A 1922985 unspecified role unspecified role prey prey purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1922986 unspecified role unspecified role bait bait purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1922984 f4