UniProt UniProt http://www.uniprot.org shibano-2014-1 xenla-embryo Xenopus laevis embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shibano T., Mamada H., Hakuno F., Takahashi S., Taira M. Origin of Nemp1 and its constructs according Mamada et al., 2009 (PMID 19167377). Origin of Ran(delta)C contrsuct according Villa Braslavsky et al., 2000 (PMID 10995230). Use of Nemp1a or Nemp1b constructs in experimental set-ups confirmed by the author (T. Shibano) by e-mail. m_taira@bs.s.u-tokyo.ac.jp PloS one 2014 imex curation Only protein-protein interactions shibano-2014-3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Shibano T., Mamada H., Hakuno F., Takahashi S., Taira M. m_taira@bs.s.u-tokyo.ac.jp mouse 11-day embryo MatchMaker cDNA library PloS one 2014 imex curation Only protein-protein interactions shibano-2014-4 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Shibano T., Mamada H., Hakuno F., Takahashi S., Taira M. Association between GST-RanQ69L and importin beta1 (and the inhibitory effect of Nemp1; fig 3F) omitted as origin of importin beta1 cannot be established from the publication. m_taira@bs.s.u-tokyo.ac.jp PloS one 2014 imex curation Only protein-protein interactions shibano-2014-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Shibano T., Mamada H., Hakuno F., Takahashi S., Taira M. m_taira@bs.s.u-tokyo.ac.jp PloS one 2014 imex curation Only protein-protein interactions shibano-2014-5 chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 confocal microscopy confocal microscopy immunostaining immunostaining Shibano T., Mamada H., Hakuno F., Takahashi S., Taira M. m_taira@bs.s.u-tokyo.ac.jp PloS one 2014 imex curation Only protein-protein interactions nmp1b_xenla Nuclear envelope integral membrane protein 1b nemp1b tmem194a-b protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAGEVEGRGCGFSLGVLVTLLVLPLPSLCTLSTEKELHVIKLYEGRMVRYNESRNFCYQRTYEPKWSDVWTKIQIRINSTKMIRVTQVDNEEKLKEMETFNMFDFFSSFLKEKLNDTFIYVNLYSNKTCVKVHLTDTDTYYSVALSRGFDPRLFFVFLCGLLLFFYGDTLSRSQLFFYSTGITVGMLASMLILVFMLSKLMPKKSPFFALLLGGWSVSIYVIQLVFRNLQAICSEYWQYLIVYLGIVGFVSFAFCYIYGPLENERSINILNWTLQLIGLLLMYVSVQIQHIAVTIVVIAFCTKQIEYPVQWIYILYRKIKLKRAKPGPPRLLTEEEYRKQADVETRKALEELRECCSSPDFAAWKTISRIQSPKRFADFVEGSSHLTPNEVSVHEHEYGLGGSFLEDELFGEDSDVEEEMEIEPPLYPIPRSVF emd_human Emerin EDMD STA EMD protein protein human Homo sapiens Human MDNYADLSDTELTTLLRRYNIPHGPVVGSTRRLYEKKIFEYETQRRRLSPPSSSAASSYSFSDLNSTRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSAYQSITHYRPVSASRSSLDLSYYPTSSSTSFMSSSSSSSSWLTRRAIRPENRAPGAGLGQDRQVPLWGQLLLFLVFVIVLFFIYHFMQAEEGNPF ran_mouse GTP-binding nuclear protein Ran GTPase Ran Ras-related nuclear protein Ras-like protein TC4 Ran Rasl2-8 protein protein mouse Mus musculus Mouse MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL nemp1_mouse Nuclear envelope integral membrane protein 1 Nemp1 Kiaa0286 Tmem194 Tmem194a protein protein mouse Mus musculus Mouse MAGGIKVSVWSAVGPGPRCWGAGGGGGATWLLLVVAGCVVCGSADVNVVMLQESQVDMNSSQQFCYKNVLIPKWHDIWTRIQVRVNSSKLVRVTQVDNEEKLKELEQFSIWNFFSSFLKEKLNDTYVNVGLYSTKTCLKVEMIEKDTTYSVTVTRRFDPKLFLVFLLGLTLFFCGDLLSRSQIFYYSTGMSVGIVASLLIVIFMISKFMPKRSPIYVILVGGWSFSLYLIQLVFKNLQEIWRSYWHYLLSYILTVGFMSFAVCYKYGPLENERSINLLTWTLQLLGLGLMYSSIQIPHVAFALIVIALCTKNLEYPIHWLCSTYRRMCKASGKPVPPRLLTEEEYRIQGEVETQKALQELREFCNSPECSAWKTISRIQSPKRFADFVEGSFHLTPNEVSVHEQEYGLGSIFTQDEELSSEEEGSEYPTFTQNNFLT ran_xenla GTP-binding nuclear protein Ran ran GTPase Ran Ras-like protein TC4 Ras-related nuclear protein protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEQDLQNAQATALPDEDDDL nmp1a_xenla Nuclear envelope integral membrane protein 1a nemp1a tmem194a-a protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAGDVEGGGCRVSWGALLTLLLLPLPSLCTLASGKEPHVIKLYEGKVVRYNESKNFCYQRTYEPKWSDVWTKIQIRVNSTKMIRVTQVENEEKLKEMETFNMFDLFSSFLKEKLNDTFIYVDLYSNKTCIKVHVIDTDTYYSVALSRGFDPRLCFLFLCGLLLFFYGDALSRSQLFFYSTGITIGMLASMLILVFMLSKLMPKKSPFVALLLGGWSVSIYIIQLVFKNLQAICSEYWQYLLGYLGIVGFVSFAFCYKYGPLENDRSINILTWTLQLIGLLLMYISVQIQHIAVTMVVIAFCTKQIEYPVQWIYILYRKIKRKRAKPSPPRLLTEEEYRKQGEIETRKALEELRGYCSSPDFATWKMISRIQSPKRFADFVEGSSHLTPNEVSVHEHEYGLGGSFLEDELFGEDSDIEVEMDIEQPLYLVPRSCF ran2_arath GTP-binding nuclear protein Ran-2 RAN2 Ras-related nuclear protein 2 F28I16.170 At5g20020 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MALPNQQTVDYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDQNLHFVESPALAPPEVHLDIAAQQQNEADLAAAAAQPLPDDDDDAFE q9shq6_arath F1K23.6 F1K23_6 At1g28760 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTTSSSFLRLPHRIWFLLPLIVFLLLSYAAALKSVGPGEPALNISLRSCERIQVSGYSRTKLGKFANSLRVNLVPIPEKLHLKIQVCVHRNATLGMCHCEKSNWKNLQSSSVISPYDKQYIDVRFTGSEYQSSRSMITVNVTEEFHQWRIFCLVAGLIVILLAPVVSSWLPFYYTSSMAVGVFLVVLIIIFQVMRLLPTGRKNVMYLAFYGSVVGAGSFILHQFSMMVNMILVNFGLSEDMYNPVAILVLVGVVITGAAFGFWTVRKFVVSKDGGGVDASVAQFVKWAMRSVAATFILQSSLDTPMAMGAFVLASLFGFLVSKTKSFVGKSHWLVSAGERRPIMHGRAEFLSRPGGGGLWKSAKSVPSYSGSPSYDVIVSSPSSVNRRIPIGNQDYYSTFHTTPNRKRMSKREYDELTQETTREAMAGLAASPGFSDWLVEHADRIKLLPTDSSYDDELGSESDSTGEQSWTRFFW f4k567_arath K9I9.18 K9I9_18 At5g67610 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGDYRLLRNSGVVIGIILLHLFTFASLVSSDELQFGVVVNFLWPYGTVVGESGELQVTPSLEVKGSPGLKPDRTSLCERIHIHGLGRFKHLDKYAHSLKLIVNASISGKTNNIDVCFHRNLSRGIGMCPHSRWEKASKGSWVQTMSPFDHKILDVRVPSSNKVSLEVSAVEELFMHRIVFLLLGAVLLASASTLSQSLAFYYSSAMAVGIILVVLLVLFQGMKLLPTGRSSFALFIYSTLLGLGGFLLRYLPGLFESLLTEMGIDEEMYTPAAIFVGAFLSLGGAFFGFWTVRKLILTEDGSIDVSTSLFVSWSIRIMAAVLILQSSVDPLLAGGALISVILMSSTLKKITRLKFLLRLFEIPLNLLLGIWEAIRDTDIPSVPGYLHDFMQKSPDASGFRNRVTSASPSGGINNGMRESPPSESDTFPSSFHKTPERSQLTKEEWKKLTKDSTTKAVQELVSSPDFGKWAAVNADRINVTPRKGSSSKNQPRKWMRWF q7ztb4_xenla lemd3.S XMAN1 lemd3 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAAAQLTDEELFSELKRSGIMPGPVTDSTRPVYLKKLKKLREEQRLRHGKVRVITNNNTVAAPSESEGILKSGGDVTHKRYSRQSLAREAGKPSPILGFSSDESDVDAGLGAHGSRRSRTNTPQPERKSPVQHQPGTADANADRRKVPGWWEMRRPRGDSEEDDDDEEEGTEEASNCRTMNGSRHSYCRDYSDTDEEEQLGQDEQASRRSRRGSSKQAKEQSLDRDELAAWPGWSREVAGVSHRHRGAARKSHTAADTDGAGNLKRKSNNHLESGYNKDPPRVYFPSNSLPPRMPQSNHTAAPNHTKYSHYHYPDSKRQHHKVAEPEEALLQQFKRDEVSAAGRFSAHYLSMFLLTAACLFFLLLGLTYLRMRGTSLPANENLTSTEDLNVDSKKNLMNTLQKLHEKLSKTAGDYICGNTDHRNISVQEAKQYLRTLGQEYENEFNNTLAWILEHSATRDIGIKCVSDPGSILNITSVKYLESTLPKMSFMCRIKRAVITVAHRSSILLVGVGVLFGVWRYIKYRWAKEEEETKQMYDMVVKIIDVLKSHNEACQENKGLEPYTPIPHVRDSLILPQDRKKMKKVWDRAVEFLDANESRVRTETQKIGGADFQVWKWIQPSSTCDKISVMPSKVWQGQAFHLDRRNSPPNSLTPCLKIRNMFDPVMEIGDHWDLAIQEAILEKCSDNEGIVHIAVDKNSREGCVYVKCLSPEFAGKAFKALHGSWFDGKLVTVKYLRLDRYHHRFPQALTCSTPLKPSNTHMNSLLHLNLRTGTSNSQSCC ubc9_mouse SUMO-conjugating enzyme UBC9 Ube2i Ubc9 Ubce9 Ubce2i SUMO-protein ligase Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I Ubiquitin carrier protein I Ubiquitin carrier protein 9 RING-type E3 SUMO transferase UBC9 protein protein mouse Mus musculus Mouse MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS emd-nemp1a-1 2866280 N/A 2866285 unspecified role unspecified role prey prey microinjection microinjection micro-injection c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 2866286 unspecified role unspecified role bait bait microinjection microinjection micro-injection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2A nemp1-ran-3 2866280 N/A 2866287 unspecified role unspecified role prey prey microinjection microinjection micro-injection P62827:p.Thr42Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P62827:p.Gln69Leu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P62827:p.Thr24Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false N/A 2866288 unspecified role unspecified role bait bait microinjection microinjection micro-injection Q6ZQE4:p.[Ser366Glu;Ser376Glu;Ser380Glu;Ser419Glu;Ser420Glu] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q6ZQE4:p.[Ser366Ala;Ser376Ala;Ser380Ala;Ser419Ala;Ser420Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3B and E, 5A, S3, 6D, 9B nemp1-ran-6 2866280 N/A 2866287 unspecified role unspecified role prey prey microinjection microinjection micro-injection c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 2866288 unspecified role unspecified role bait bait microinjection microinjection micro-injection c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 4A nemp1a-ran-1 2866280 N/A 2866289 unspecified role unspecified role prey prey microinjection microinjection micro-injection n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 2866290 unspecified role unspecified role bait bait microinjection microinjection micro-injection c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 4B q9shq6_arath-ran2-1 2866280 N/A 2866291 unspecified role unspecified role prey prey microinjection microinjection micro-injection n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 2866292 unspecified role unspecified role bait bait microinjection microinjection micro-injection n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 9A and B f4k567_arath-ran2-1 2866280 N/A 2866293 unspecified role unspecified role bait bait microinjection microinjection micro-injection n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 2866291 unspecified role unspecified role prey prey microinjection microinjection micro-injection n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 9A nemp1a-nemp1b-1 2866280 N/A 2866285 unspecified role unspecified role prey prey microinjection microinjection micro-injection c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 2866290 unspecified role unspecified role bait bait microinjection microinjection micro-injection c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2A and B lemd3-nemp1b-1 2866280 N/A 2866294 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2866285 unspecified role unspecified role prey prey microinjection microinjection micro-injection c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 2A nemp1-ran-2 2866281 N/A 2866287 unspecified role unspecified role prey prey N/A 2866288 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false no figure nemp1-ran-8 2866281 N/A 2866295 unspecified role unspecified role prey prey N/A 2866288 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false no figure nemp1-ran-4 2866282 N/A 2866287 unspecified role unspecified role prey prey microinjection microinjection micro-injection c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P62827:p.Gln69Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false P62827:p.Thr24Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false xenla-embryo Xenopus laevis embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. N/A 2866288 unspecified role unspecified role bait bait microinjection microinjection micro-injection n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 3C nemp1-ran-5 2866282 N/A 2866287 unspecified role unspecified role prey prey microinjection microinjection micro-injection purified purified n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P62827:p.Gln69Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 2866288 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 3D GTP was present in the buffer. nemp1-ran-1 2866283 N/A 2866287 unspecified role unspecified role prey prey N/A 2866288 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3A nemp1-ran-7 2866284 N/A 2866287 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 2866288 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false 5B