UniProt UniProt http://www.uniprot.org buschmann-2015-5 tobac-leaf Nicotiana tabacum (tobacco) leaf leaf leaf confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Buschmann H., Dols J., Kopischke S., Peña EJ., Andrade-Navarro MA., Heinlein M., Szymanski DB., Zachgo S., Doonan JH., Lloyd CW. henrik.buschmann@biologie.uni-osnabrueck.de Journal of cell science 2015 imex curation Only protein-protein interactions buschmann-2015-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Buschmann H., Dols J., Kopischke S., Peña EJ., Andrade-Navarro MA., Heinlein M., Szymanski DB., Zachgo S., Doonan JH., Lloyd CW. henrik.buschmann@biologie.uni-osnabrueck.de Arabidopsis seedling library Journal of cell science 2015 imex curation Only protein-protein interactions buschmann-2015-3 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Buschmann H., Dols J., Kopischke S., Peña EJ., Andrade-Navarro MA., Heinlein M., Szymanski DB., Zachgo S., Doonan JH., Lloyd CW. henrik.buschmann@biologie.uni-osnabrueck.de Journal of cell science 2015 imex curation Only protein-protein interactions buschmann-2015-2 arath-cell-culture Arabidopsis thaliana cell culture plant_cell_culture plant cellular culture The culture conditions should always be cited in the experimental modifications annotation Either whole plant or undifferentiated plant cells maintained in culture. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Buschmann H., Dols J., Kopischke S., Peña EJ., Andrade-Navarro MA., Heinlein M., Szymanski DB., Zachgo S., Doonan JH., Lloyd CW. henrik.buschmann@biologie.uni-osnabrueck.de Journal of cell science 2015 imex curation Only protein-protein interactions buschmann-2015-4 tobac-by_2 Tobacco cell line by_2 Tobacco cell line An undifferentiated cell line derived from tobacco callus tissue. An undifferentiated cell line derived from tobacco callus tissue. confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Buschmann H., Dols J., Kopischke S., Peña EJ., Andrade-Navarro MA., Heinlein M., Szymanski DB., Zachgo S., Doonan JH., Lloyd CW. henrik.buschmann@biologie.uni-osnabrueck.de Journal of cell science 2015 imex curation Only protein-protein interactions kn14e_arath Kinesin-like protein KIN-14E KIN14E KCBP Kinesin-like calmodulin-binding protein Protein ZWICHEL ZWI K14B20.10 At5g65930 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEGQRGSNSSLSSGNGTEVATDVSSCFYVPNPSGTDFDAESSSLPPLSPAPQVALSIPAELAAAIPLIDRFQVEAFLRLMQKQIQSAGKRGFFYSKKSSGSNVRERFTFEDMLCFQKDPIPTSLLKINSDLVSRATKLFHLILKYMGVDSSDRSTPPSLDERIDLVGKLFKKTLKRVELRDELFAQISKQTRHNPDRQYLIKAWELMYLCASSMPPSKDIGGYLSEYIHNVAHDATIEPDAQVLAVNTLKALKRSIKAGPRHTTPGREEIEALLTGRKLTTIVFFLDETFEEISYDMATTVSDAVEELAGTIKLSAFSSFSLFECRKVVSSSKSSDPGNEEYIGLDDNKYIGDLLAEFKAIKDRNKGEILHCKLVFKKKLFRESDEAVTDLMFVQLSYVQLQHDYLLGNYPVGRDDAAQLCALQILVGIGFVNSPESCIDWTSLLERFLPRQIAITRAKREWELDILARYRSMENVTKDDARQQFLRILKALPYGNSVFFSVRKIDDPIGLLPGRIILGINKRGVHFFRPVPKEYLHSAELRDIMQFGSSNTAVFFKMRVAGVLHIFQFETKQGEEICVALQTHINDVMLRRYSKARSAANSLVNGDISCSSKPQNFEVYEKRLQDLSKAYEESQKKIEKLMDEQQEKNQQEVTLREELEAIHNGLELERRKLLEVTLDRDKLRSLCDEKGTTIQSLMSELRGMEARLAKSGNTKSSKETKSELAEMNNQILYKIQKELEVRNKELHVAVDNSKRLLSENKILEQNLNIEKKKKEEVEIHQKRYEQEKKVLKLRVSELENKLEVLAQDLDSAESTIESKNSDMLLLQNNLKELEELREMKEDIDRKNEQTAAILKMQGAQLAELEILYKEEQVLRKRYYNTIEDMKGKIRVYCRIRPLNEKESSEREKQMLTTVDEFTVEHPWKDDKRKQHIYDRVFDMRASQDDIFEDTKYLVQSAVDGYNVCIFAYGQTGSGKTFTIYGHESNPGLTPRATKELFNILKRDSKRFSFSLKAYMVELYQDTLVDLLLPKSARRLKLEIKKDSKGMVFVENVTTIPISTLEELRMILERGSERRHVSGTNMNEESSRSHLILSVVIESIDLQTQSAARGKLSFVDLAGSERVKKSGSAGCQLKEAQSINKSLSALGDVIGALSSGNQHIPYRNHKLTMLMSDSLGGNAKTLMFVNVSPAESNLDETYNSLLYASRVRTIVNDPSKHISSKEMVRLKKLVAYWKEQAGKKGEEEDLVDIEEDRTRKDEADS air9_arath 187-kDa microtubule-associated protein AIR9 Auxin-induced in root cultures protein 9 T29F13 At2g34680 AIR9 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEVAAKVEEETVETNVDAVKEDNATIANESRSPESVSAVSVVSNRAASTKKKPVISSNLIKPTASSSLRVSGTTPVTIRRNSTGGVTENLAGTSKVLPKQVSTTASRTDPVRRSLPELRKSSVSSLSAKTVSKPSLSESKKSVPVSPGSRSLTKSTGFSLSKPESSARPAMSVSVSSKRAPSSSVDSSGSRTSSGRLHSTLTSGRTVSKVSSPSAGSSPSVSSSIRSKSFSSPLDRTSNFSGRKKTSTPESRDSRLIILPKVEVKAGDDMRLDLRGHRIRSLTSGGLHLSPNLEFVYLRDNLLSTLEGIEILNRVKVLDLSFNDFKGPGFEPLENCKMLQQLYLAGNQITSLASLPQLPNLEFLSVAQNKLKSLAMASQPRLQVLAASKNKITTLKDFPYLPVLEHLRVEENPLLKISHLEAASILLVGPTLKKFNDRDLSREEVAIAKRYPPQTALCLREGWEFCKSDLAAESTFRFLVERWKDTLPSGYLIKEAHVDRPSEEAPCQCHFGLFQESPTATDQELALKFQWSVADRSLSNFVPILNATKEVYWPKREDIGKILKIECTPVMAETEYPSIFAISSPVQRGKGIPKVVSLELNGELVEGNIIKGQAVVAWCGGTPGKCITSWLRRKWNGSPVVIDGAEDEEYMLSLDDVGSSMVFMYTPVTEGGARGEPQYKYTEFVKAAPPSVSNVRITGDAVEGCVLKGVGDYFGGKEGPSKFEWLRKNKETGELSLISAGTSEYTLTQEDVGTHVTFVYIPANFEGLEGEPVSTSSSVVKPAPPKVTDAKIVGDLRENSKVTVTGTVTGGTEGSSRVQWFKSSCSILEGDNSLEELSTSKVAKSFRIPLGAVGYYIVAKYTPMTPDGECGEPVYVLSERAVETLPPSLNFLSITGDNIEGGILTASYGYIGGHEGKSKYEWHYHKAENDLPGALIPEASGLLQYTITKEAIGKFISFQCIPVRDDGIVGEPRSCMSQERVRPGNPSTVSLHVVGALVEGTMLSAEKEYWGGEEGASVFRWFRTNSDGTPCEIKGATTSSYLLSVGDIGYFISVSYEPVRNDRARGPTAISEIAGPIVAGHPNCQSLEFLGSMIEGQRLSFVASYTGGMKGNCYLEWVRVKNNGVKEILSSDEFLDLSLDDVGESIELIYTPVREDGIEGSPRSIRTDGIAPANPMGLELLIPDCCEKQEVVPHKTYFGGHEGVGEYIWYRTKVKLHGSALTEISYAGEEVVVCCRTLKYTPSLEDVGAYLVLYWIPTRVDGRSGKPVVVITNSPVAPADPEVSNVRVKKLFSDAYSGEGEYFGGHEGPSLFSWYRENDGTIDLIDGANSKTYEVTESDYNCRILFGYTPVRSDSVVGELKMSEPTEIILPEVPKVDMLAFTGKAVQGDVLTAVQVIPKTEIQQLVWSKYKGDIQYQWFRSPESGDKISYEALSSEISCSYKVRFEDIGRCLKCECVVHDVFGRSSELAYAETDPISPGFPRIEKLEIEGQGFHTNLYAVRGNYFGGKEGKSKIQWLRSMVGSPDLISIPGETGRMYEANVDDVGYRLVVVYTPIREDGVQGHPVSASTEPVAVEPDILKEVRQKLETGLVKFEVLCDKDPYPKKIVGEGNLERRMLEMNRKRIKVVKPGSKTSFATTEVRGSYGPPFHVETFRNDQRRLRIVVDSENEVDIVVQSRHLRDVIVLVIRGFAQRFNSTSLNSLLKIDT air9-kcbp-5 2846250 N/A 2846255 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2846256 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. 3E air9-kcbp-6 2846250 N/A 2846255 unspecified role unspecified role neutral component neutral component c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 2846256 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig S3F air9-kcbp-1 2846251 N/A 2846255 unspecified role unspecified role prey prey N/A 2846256 unspecified role unspecified role bait bait A9 repeats region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false (Fig.2A) air9-kcbp-3 2846252 N/A 2846255 unspecified role unspecified role prey prey purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 2846256 unspecified role unspecified role bait bait purified purified A9 repeats region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig.2C A smaller ∼50 kDa N-terminal truncation product of the His–AIR9 fragment also interacted with GST–KCBP. air9-kcbp-2 2846253 N/A 2846255 unspecified role unspecified role prey prey N/A 2846256 unspecified role unspecified role bait bait physical association physical association false false false Fig.2B air9-kcbp-4 2846254 N/A 2846255 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2846256 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig.2D