IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ dutta-2014-5 human-t47d-a18 ER- and PR-positive hormone-responsive clone of t47d t47d-a18 ER- and PR-positive hormone-responsive clone of t47d fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Dutta S., Bandyopadhyay C., Bottero V., Veettil MV., Wilson L., Pins MR., Johnson KE., Warshall C., Chandran B. "rANG" is of Human origin - see Sadagopan et al., 2009. sujoy.dutta@rosalindfranklin.edu Molecular oncology 2014 Only protein-protein interactions imex curation imex curation dutta-2014-2 human-t47d-a18 ER- and PR-positive hormone-responsive clone of t47d t47d-a18 ER- and PR-positive hormone-responsive clone of t47d anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Dutta S., Bandyopadhyay C., Bottero V., Veettil MV., Wilson L., Pins MR., Johnson KE., Warshall C., Chandran B. "rANG" is of Human origin - see Sadagopan et al., 2009. sujoy.dutta@rosalindfranklin.edu Molecular oncology 2014 Only protein-protein interactions imex curation imex curation dutta-2014-3 human-t47d-a18 ER- and PR-positive hormone-responsive clone of t47d t47d-a18 ER- and PR-positive hormone-responsive clone of t47d pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Dutta S., Bandyopadhyay C., Bottero V., Veettil MV., Wilson L., Pins MR., Johnson KE., Warshall C., Chandran B. "rANG" is of Human origin - see Sadagopan et al., 2009. sujoy.dutta@rosalindfranklin.edu Molecular oncology 2014 Only protein-protein interactions imex curation imex curation dutta-2014-1 human-t47d-a18 ER- and PR-positive hormone-responsive clone of t47d t47d-a18 ER- and PR-positive hormone-responsive clone of t47d anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Dutta S., Bandyopadhyay C., Bottero V., Veettil MV., Wilson L., Pins MR., Johnson KE., Warshall C., Chandran B. Cell surface biotinylated proteins were isolated and purified by passing cellular lysates through a monomeric avidin column prior to IP. "rANG" is of Human origin - see Sadagopan et al., 2009. sujoy.dutta@rosalindfranklin.edu Molecular oncology 2014 Only protein-protein interactions imex curation imex curation dutta-2014-4 human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Dutta S., Bandyopadhyay C., Bottero V., Veettil MV., Wilson L., Pins MR., Johnson KE., Warshall C., Chandran B. "rANG" is of Human origin - see Sadagopan et al., 2009. sujoy.dutta@rosalindfranklin.edu Molecular oncology 2014 Only protein-protein interactions imex curation imex curation s10aa_human Protein S100-A10 S100 calcium-binding protein A10 Calpactin-1 light chain Calpactin I light chain p10 protein p11 Cellular ligand of annexin II S100A10 ANX2LG CAL1L CLP11 protein protein human Homo sapiens Human MPSQMEHAMETMMFTFHKFAGDKGYLTKEDLRVLMEKEFPGFLENQKDPLAVDKIMKDLDQCRDGKVGFQSFFSLIAGLTIACNDYFVVHMKQKGKK angi_human Angiogenin Ribonuclease 5 ANG RNASE5 protein protein human Homo sapiens Human MVMGLGVLLLVFVLGLGLTPPTLAQDNSRYTHFLTQHYDAKPQGRDDRYCESIMRRRGLTSPCKDINTFIHGNKRSIKAICENKNGNPHRENLRISKSSFQVTTCKLHGGSPWPPCQYRATAGFRNVVVACENGLPVHLDQSIFRRP anxa2_human Annexin A2 ANXA2 ANX2 ANX2L4 CAL1H LPC2D Annexin-2 Annexin II Lipocortin II Calpactin I heavy chain Chromobindin-8 p36 Protein I Placental anticoagulant protein IV Calpactin-1 heavy chain protein protein human Homo sapiens Human MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD upar_human Urokinase plasminogen activator surface receptor Monocyte activation antigen Mo3 PLAUR MO3 UPAR protein protein human Homo sapiens Human MGHPPLLPLLLLLHTCVPASWGLRCMQCKTNGDCRVEECALGQDLCRTTIVRLWEEGEELELVEKSCTHSEKTNRTLSYRTGLKITSLTEVVCGLDLCNQGNSGRAVTYSRSRYLECISCGSSDMSCERGRHQSLQCRSPEEQCLDVVTHWIQEGEEGRPKDDRHLRGCGYLPGCPGSNGFHNNDTFHFLKCCNTTKCNEGPILELENLPQNGRQCYSCKGNSTHGCSSEETFLIDCRGPMNQCLVATGTHEPKNQSYMVRGCATASMCQHAHLGDAFSMNHIDVSCCTKSGCNHPDLDVQYRSGAAPQPGPAHLSLTITLLMTARLWGGTLLWT urok_human Urokinase-type plasminogen activator PLAU protein protein human Homo sapiens Human MRALLARLLLCVLVVSDSKGSNELHQVPSNCDCLNGGTCVSNKYFSNIHWCNCPKKFGGQHCEIDKSKTCYEGNGHFYRGKASTDTMGRPCLPWNSATVLQQTYHAHRSDALQLGLGKHNYCRNPDNRRRPWCYVQVGLKPLVQECMVHDCADGKKPSSPPEELKFQCGQKTLRPRFKIIGGEFTTIENQPWFAAIYRRHRGGSVTYVCGGSLISPCWVISATHCFIDYPKKEDYIVYLGRSRLNSNTQGEMKFEVENLILHKDYSADTLAHHNDIALLKIRSKEGRCAQPSRTIQTICLPSMYNDPQFGTSCEITGFGKENSTDYLYPEQLKMTVVKLISHRECQQPHYYGSEVTTKMLCAADPQWKTDSCQGDSGGPLVCSLQGRMTLTGIVSWGRGCALKDKPGVYTRVSHFLPWIRSHTKEENGLAL rata5yea4-1 2825363 N/A 2825368 unspecified role unspecified role neutral component neutral component N/A 2825369 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3B,4B, 4C. ang-s100a10-1 2825363 N/A 2825370 unspecified role unspecified role neutral component neutral component N/A 2825368 unspecified role unspecified role neutral component neutral component N/A 2825369 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4B ang-anxa2-5 2825363 N/A 2825370 unspecified role unspecified role neutral component neutral component N/A 2825369 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4Bi ang-anxa2-6 2825363 N/A 2825370 unspecified role unspecified role neutral component neutral component N/A 2825368 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4Bii ang-anxa2-7 2825363 N/A 2825371 unspecified role unspecified role neutral component neutral component N/A 2825368 unspecified role unspecified role neutral component neutral component N/A 2825369 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4C ang-plaur-1 2825363 N/A 2825371 unspecified role unspecified role neutral component neutral component N/A 2825369 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4Ci ang-plaur-2 2825363 N/A 2825371 unspecified role unspecified role neutral component neutral component N/A 2825368 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4Cii ang-anxa2-1 2825364 N/A 2825371 unspecified role unspecified role prey prey N/A 2825370 unspecified role unspecified role prey prey N/A 2825368 unspecified role unspecified role prey prey N/A 2825369 unspecified role unspecified role bait bait association association false false false 6Ai s100a10-ang-1 2825364 N/A 2825370 unspecified role unspecified role prey prey N/A 2825368 unspecified role unspecified role bait bait N/A 2825369 unspecified role unspecified role prey prey association association false false false 6Aii ang-anxa2-2 2825364 N/A 2825370 unspecified role unspecified role bait bait N/A 2825368 unspecified role unspecified role prey prey N/A 2825369 unspecified role unspecified role prey prey association association false false false 6Aiii ang-anxa2-3 2825364 N/A 2825371 unspecified role unspecified role bait bait N/A 2825369 unspecified role unspecified role prey prey physical association physical association false false false 6Aiv plau-plaur-1 2825364 N/A 2825369 unspecified role unspecified role ancillary ancillary N/A 2825372 unspecified role unspecified role bait bait N/A 2825371 unspecified role unspecified role prey prey physical association physical association false false false 11B ang-anxa2-4 2825365 N/A 2825369 unspecified role unspecified role neutral component neutral component N/A 2825368 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 6C-4 s100a10-ang-2 2825365 N/A 2825369 unspecified role unspecified role neutral component neutral component N/A 2825368 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 6C-2 anxa2-ang-1 2825365 N/A 2825370 unspecified role unspecified role neutral component neutral component N/A 2825369 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 6C-3 plaur-ang-1 2825365 N/A 2825371 unspecified role unspecified role neutral component neutral component N/A 2825369 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 6C-1 76876yuoio-1 2825366 N/A 2825370 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2825368 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2825369 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5C e8ditcg-1 2825367 N/A 2825369 unspecified role unspecified role ancillary ancillary N/A 2825372 unspecified role unspecified role bait bait N/A 2825371 unspecified role unspecified role prey prey physical association physical association false false false 11A, 13D The addition of recombinant ANG restores the interaction lost in ANG-/- cells.