UniProt UniProt http://www.uniprot.org oda-2013-2 tobac-leaf Nicotiana tabacum (tobacco) leaf leaf leaf bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Oda Y., Fukuda H. oda@biol.s.u-tokyo.ac.jp The Plant cell 2013 imex curation Only protein-protein interactions oda-2013-3 arath-cell-culture Arabidopsis thaliana cell culture plant_cell_culture plant cellular culture The culture conditions should always be cited in the experimental modifications annotation Either whole plant or undifferentiated plant cells maintained in culture. confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Oda Y., Fukuda H. Arabidopsis differentiating xylem cells (Fig. 3A). Arabidopsis non-xylem cultured cells (Fig.8). oda@biol.s.u-tokyo.ac.jp The Plant cell 2013 imex curation Only protein-protein interactions oda-2013-4 arath-cell-culture Arabidopsis thaliana cell culture plant_cell_culture plant cellular culture The culture conditions should always be cited in the experimental modifications annotation Either whole plant or undifferentiated plant cells maintained in culture. bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Oda Y., Fukuda H. Arabidopsis differentiating xylem cells oda@biol.s.u-tokyo.ac.jp The Plant cell 2013 imex curation Only protein-protein interactions icr5_arath Interactor of constitutive active ROPs 5 ICR5 MIDD1 Protein MICROTUBULE DEPLETION DOMAIN 1 RIP3 ROP-interactive partner 3 F4P12.50 At3g53350 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQTPKSRPGSLELPQKKSPLPAPKVVRRLKPSGAESDPKTKTISKTQIPKVVADRRSARIPLNEIQKKRTGRIPELESTISQLQEELKKAKEELNRSEALKREAQEEAEDAKHQLMDINASEDSRIEELRKLSQERDKTWQSELEAMQRQHGMDSTALSSAINEVQKLKSKLFESESELEQSKYEVRSLEKLVRQLEEERVNSRDSSSSMEVEELKEAMNLSRQEITQLKSAVEAAETRYQEEYIQSTLQIRSAYEQTEAVKSRYSQREAELTEELNRTKDEIEGLRKELMEKVKEDESTGDLKKLESDLMEVRGSLMDKEMELQILRSAMEKKVETANTEAMEAELKRVKIQCEQWRKAAETAASILNNDEERTDSIETSKMLKKFGVLLKKNHK kn13a_arath Kinesin-like protein KIN-13A KIN13A AtKINESIN-13A KINESIN-13A MGL6.9 At3g16630 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGGQMQQNNAAAATALYDGALPTNDAGDAVMARWLQSAGLQHLASPVASTGNDQRHLPNLLMQGYGAQTAEEKQRLFQLMRNLNFNGESTSESYTPTAHTSAAMPSSEGFFSPEFRGDFGAGLLDLHAMDDTELLSEHVITEPFEPSPFMPSVNKEFEEDYNLAANRQQRQQTEAEPLGLLPKSDKENNSVAKIKVVVRKRPLNKKETAKKEEDVVTVSDNSLTVHEPRVKVDLTAYVEKHEFCFDAVLDEDVSNDEVYRATIEPIIPIIFQRTKATCFAYGQTGSGKTFTMKPLPIRAVEDLMRLLRQPVYSNQRFKLWLSYFEIYGGKLFDLLSERKKLCMREDGRQQVCIVGLQEYEVSDVQIVKDFIEKGNAERSTGSTGANEESSRSHAILQLVVKKHVEVKDTRRRNNDSNELPGKVVGKISFIDLAGSERGADTTDNDRQTRIEGAEINKSLLALKECIRALDNDQLHIPFRGSKLTEVLRDSFVGNSRTVMISCISPNAGSCEHTLNTLRYADRVKSLSKSGNSKKDQTANSMPPVNKDPLLGPNDVEDVFEPPQEVNVPETRRRVVEKDSNSSTSGIDFRQPTNYREESGIPSFSMDKGRSEPNSSFAGSTSQRNNISSYPQETSDREEKVKKVSPPRGKGLREEKPDRPQNWSKRDVSSSDIPTLTNFRQNASETASRQYETASRQYETDPSLDENLDALLEEEEALIAAHRKEIEDTMEIVREEMKLLAEVDQPGSMIENYVTQLSFVLSRKAAGLVSLQARLARFQHRLKEQEILSRKRVPR icr5-kin13a-1 2824129 N/A 2824132 unspecified role unspecified role neutral component neutral component region cyfp cYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2824133 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region nyfp nYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3B kin13a-irc5-2 2824130 N/A 2824132 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2824133 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false Fig. 3A, Fig. 8 MIDD1 and Kinesin-13A delta_M colocalized in the membrane domains underlying secondary cell wall pits. icr5-kinesin_13a-1 2824131 N/A 2824132 unspecified role unspecified role neutral component neutral component region cyfp cYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2824133 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region nyfp nYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3C The YFP fluorescent signal was detected in the secondary cell wall pits.