IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ swinehart-2013-1 in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as autoradiography autoradiography Swinehart WE., Henderson JC., Jackman JE. The reaction was performed in the precence of 0.5mM SAM The p methyl G was resolved from (unmethylated) p G by TLC and visualized by autoradiography. jackman.14@osu.edu RNA (New York, N.Y.) 2013 imex curation Only protein-protein interactions imex curation swinehart-2013-2 in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as primer extension assay primer extension assay primer extension assay Swinehart WE., Henderson JC., Jackman JE. jackman.14@osu.edu RNA (New York, N.Y.) 2013 imex curation Only protein-protein interactions imex curation trna_gly_gcc tRNA_Gly_GCC trna transfer rna transfer ribonucleic acid tRNA transfer RNA trna yeast Saccharomyces cerevisiae Baker's yeast GCGCAAGUGGUUUAGUGGUAAAAUCCAACGUUGCCAUCGUUGGGCCCCCGGUUCGAUUCCGGGCUUGCGCA trm10_yeast tRNA (guanine(9)-N1)-methyltransferase TRM10 tRNA methyltransferase 10 tRNA(m1G9)-methyltransferase O0926 YOL093W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNDEINQNEEKVKRTPPLPPVPEGMSKKQWKKMCKRQRWEENKAKYNAERRVKKKRLRHERSAKIQEYIDRGEEVPQELIREPRINVNQTDSGIEIILDCSFDELMNDKEIVSLSNQVTRAYSANRRANHFAEIKVAPFDKRLKQRFETTLKNTNYENWNHFKFLPDDKIMFGDEHISKDKIVYLTADTEEKLEKLEPGMRYIVGGIVDKNRYKELCLKKAQKMGIPTRRLPIDEYINLEGRRVLTTTHVVQLMLKYFDDHNWKNAFESVLPPRKLDAEAKSASSSPAPKDT trna val uac trna Val UAC trna transfer rna transfer ribonucleic acid tRNA transfer RNA trna yeast Saccharomyces cerevisiae Baker's yeast GGUCCAAUGGUCCAGUGGUUCAAGACGUCGCCUUUACACGGCGAAGAUCCCGAGUUCGAACCUCGGUUGGAUCA tm10a_human tRNA methyltransferase 10 homolog A tRNA (guanine(9)-N(1))-methyltransferase TRMT10A TRMT10A RG9MTD2 RNA (guanine-9-)-methyltransferase domain-containing protein 2 protein protein human Homo sapiens Human MSSEMLPAFIETSNVDKKQGINEDQEESQKPRLGEGCEPISKRQMKKLIKQKQWEEQRELRKQKRKEKRKRKKLERQCQMEPNSDGHDRKRVRRDVVHSTLRLIIDCSFDHLMVLKDIKKLHKQIQRCYAENRRALHPVQFYLTSHGGQLKKNMDENDKGWVNWKDIHIKPEHYSELIKKEDLIYLTSDSPNILKELDESKAYVIGGLVDHNHHKGLTYKQASDYGINHAQLPLGNFVKMNSRKVLAVNHVFEIILEYLETRDWQEAFFTILPQRKGAVPTDKACESASHDNQSVRMEEGGSDSDSSEEEYSRNELDSPHEEKQDKENHTESTVNSLPH trna cys gca trna Cys GCA trna transfer rna transfer ribonucleic acid tRNA transfer RNA trna yeast Saccharomyces cerevisiae Baker's yeast GCUCGUAUGGCGCAGUGGUAGCGCAGCAGAUUGCAAAUCUGUUGGUCCUUAGUUCGAUCCUGAGUGCGAGCU trna phe gaa trna Phe GAA trna transfer rna transfer ribonucleic acid tRNA transfer RNA trna yeast Saccharomyces cerevisiae Baker's yeast GCGGAUUUAGCUCAGUUGGGAGAGCGCCAGACUGAAGAUCUGGAGGUCCUGUGUUCGAUCCACAGAAUUCGCA trna asn guu trna asn GUU trna transfer rna transfer ribonucleic acid tRNA transfer RNA trna yeast Saccharomyces cerevisiae Baker's yeast GACUCCAUGGCCAAGUUGGUUAAGGCGUGCGACUGUUAAUCGCAAGAUCGUGAGUUCAACCCUCACUGGGGUCG trm10-trnaglygcc-2 1936397 N/A 1936399 enzyme target enzyme target substrate substrate neutral component neutral component N1 Methyl G rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false label 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain false N/A 1936400 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false 1936397 1936397 Fig 1A, Supp fig 1 trm10-trna val uac-1 1936397 N/A 1936401 enzyme target enzyme target substrate substrate neutral component neutral component N1 methyl G rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false label 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain false N/A 1936400 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false 1936397 1936397 Fig 1A, supp fig 1 trmt10a-trnaglygcc-1 1936397 N/A 1936399 enzyme target enzyme target substrate substrate neutral component neutral component N1 Methyl G rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false label 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain false N/A 1936402 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false 1936397 1936397 Fig 1B, Supp fig 1 trmt10a-trna val uac-1 1936397 N/A 1936402 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) N/A 1936401 enzyme target enzyme target substrate substrate neutral component neutral component N1 methyl G rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false label 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain false methylation methylation reaction methylation false false false 1936397 1936397 Fig 1B, Supp fig 1 trm10-trnaglygcc-4 1936397 N/A 1936399 enzyme target enzyme target substrate substrate neutral component neutral component N1 Methyl G rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1936400 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false Fig 5 trm10-trna cys gca-2 1936397 N/A 1936403 enzyme target enzyme target substrate substrate neutral component neutral component N1 Methyl G rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1936400 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false Fig 5 trm10-trna phe gaa-1 1936397 N/A 1936404 enzyme target enzyme target substrate substrate neutral component neutral component a9g mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain false N1 Methyl G rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1936400 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false Fig 5 trm10-trna asn guu-1 1936397 N/A 1936405 enzyme target enzyme target substrate substrate neutral component neutral component label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N1 Methyl G rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false N/A 1936400 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false Fig 5 trm10-trna val uac-3 1936398 N/A 1936401 enzyme target enzyme target substrate substrate neutral component neutral component N1 methyl G rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false yeast Saccharomyces cerevisiae Baker's yeast N/A 1936400 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false Fig 3 trm10-trnaglygcc-3 1936398 N/A 1936399 enzyme target enzyme target substrate substrate neutral component neutral component N1 Methyl G rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false yeast Saccharomyces cerevisiae Baker's yeast N/A 1936400 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false Fig 3 trm10-trna cys gca-1 1936398 N/A 1936403 enzyme target enzyme target substrate substrate neutral component neutral component N1 Methyl G rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false yeast Saccharomyces cerevisiae Baker's yeast N/A 1936400 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false Fig 3