UniProt UniProt http://www.uniprot.org morimoto-2012-1 mouse-testis Mus musculus testis testis anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Morimoto A., Shibuya H., Zhu X., Kim J., Ishiguro K., Han M., Watanabe Y. ywatanab@iam.u-tokyo.ac.jp The Journal of cell biology 2012 imex curation Only protein-protein interactions morimoto-2012-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Morimoto A., Shibuya H., Zhu X., Kim J., Ishiguro K., Han M., Watanabe Y. ywatanab@iam.u-tokyo.ac.jp The Journal of cell biology 2012 imex curation Only protein-protein interactions morimoto-2012-3 mouse-spermatocyte Mus musculus spermatocyte Spermatocyte fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Morimoto A., Shibuya H., Zhu X., Kim J., Ishiguro K., Han M., Watanabe Y. ywatanab@iam.u-tokyo.ac.jp The Journal of cell biology 2012 imex curation Only protein-protein interactions morimoto-2012-4 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Morimoto A., Shibuya H., Zhu X., Kim J., Ishiguro K., Han M., Watanabe Y. ywatanab@iam.u-tokyo.ac.jp mouse testis cDNA The Journal of cell biology 2012 imex curation Only protein-protein interactions morimoto-2012-5 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Morimoto A., Shibuya H., Zhu X., Kim J., Ishiguro K., Han M., Watanabe Y. ywatanab@iam.u-tokyo.ac.jp The Journal of cell biology 2012 imex curation Only protein-protein interactions sun1_mouse SUN domain-containing protein 1 Sun1 Unc84a Protein unc-84 homolog A Sad1/unc-84 protein-like 1 protein protein mouse Mus musculus Mouse MDFSRLHTYTPPQCVPENTGYTYALSSSYSSDALDFETEHKLEPVFDSPRMSRRSLRLVTTASYSSGDSQAIDSHISTSRATPAKGRETRTVKQRRSASKPAFSINHLSGKGLSSSTSHDSSCSLRSATVLRHPVLDESLIREQTKVDHFWGLDDDGDLKGGNKAATQGNGELAAEVASSNGYTCRDCRMLSARTDALTAHSAIHGTTSRVYSRDRTLKPRGVSFYLDRTLWLAKSTSSSFASFIVQLFQVVLMKLNFETYKLKGYESRAYESQSYETKSHESEAHLGHCGRMTAGELSRVDGESLCDDCKGKKHLEIHTATHSQLPQPHRVAGAMGRLCIYTGDLLVQALRRTRAAGWSVAEAVWSVLWLAVSAPGKAASGTFWWLGSGWYQFVTLISWLNVFLLTRCLRNICKVFVLLLPLLLLLGAGVSLWGQGNFFSLLPVLNWTAMQPTQRVDDSKGMHRPGPLPPSPPPKVDHKASQWPQESDMGQKVASLSAQCHNHDERLAELTVLLQKLQIRVDQVDDGREGLSLWVKNVVGQHLQEMGTIEPPDAKTDFMTFHHDHEVRLSNLEDVLRKLTEKSEAIQKELEETKLKAGSRDEEQPLLDRVQHLELELNLLKSQLSDWQHLKTSCEQAGARIQETVQLMFSEDQQGGSLEWLLEKLSSRFVSKDELQVLLHDLELKLLQNITHHITVTGQAPTSEAIVSAVNQAGISGITEAQAHIIVNNALKLYSQDKTGMVDFALESGGGSILSTRCSETYETKTALLSLFGVPLWYFSQSPRVVIQPDIYPGNCWAFKGSQGYLVVRLSMKIYPTTFTMEHIPKTLSPTGNISSAPKDFAVYGLETEYQEEGQPLGRFTYDQEGDSLQMFHTLERPDQAFQIVELRVLSNWGHPEYTCLYRFRVHGEPIQ kash5_mouse Protein KASH5 Kash5 Ccdc155 Coiled-coil domain-containing protein 155 KASH domain-containing protein 5 protein protein mouse Mus musculus Mouse MHSILRSSLSSREALRMRQLKGLRKERPGRHPLGVRLRAIWTSFLFPNPPHSGGKLRASTAAVEEHQEWSMDLPEGQAGGPTAQMYLWEQPEEASSRPLLSLEEQILNSTFEACDPHKTGTVTVAHLLAYLEAVTGQGPQDVRLQTLARSLDPYGEGAGATVELDTFLVVMRDWIAACQLQGGLERAEETAYEGALASPHLPSVCPEAEESANLESFGGEDPRPEGPATAELLSNLEDLELSNRRLAGENAKLQRSVETAEEGSARLGEEITALRKQLRSTQQALQVAKALDEELEDLKTLAKSLEEQNRSLMAQARHTEKEQQHLAAEVETLQEENEKLLAERDGVKRRSEELATEKDALKRQLCECERLICQREAVLSERTRHAESLARTLEEYRTTTQELRQEISNLEEQLSQSQEGPEELLEGAEAGRVGWIMALPPSLDLEIQAIRQEQDVASAGLSSPLYGVWQWEEVEPEPEPEPEPEPEPEPQEVEFPSEDPARQQTDLQREPVRALEGSRAPCLRLSRSQEEEEEEEESWVLADPSSPLGTYHHKLAPGSSRESCHIVPEMHQALMPVVRDLVPVERSRTQHCLHPQHSPGIRISQHPLVPTPVLGLLLLLLLSILLFSQSPPPTWPHLQLYYLQPPPV dctn1_mouse Dynactin subunit 1 150 kDa dynein-associated polypeptide DAP-150 p150-glued Dctn1 protein protein mouse Mus musculus Mouse MAQSRRHMSSRTPSGSRMSTEASARPLRVGSRVEVIGKGHRGTVAYVGATLFATGKWVGVILDEAKGKNDGTVQGRKYFTCDEGHGIFVRQSQIQVFEDGADTTSPETPDSSASKVLKREGADAAAKTSKLRGLKPKKAPTARKTTTRRPKPTRPASTGVAGPSSSLGPSGSASAGELSSSEPSTPAQTPLAAPIIPTPALTSPGAAPPLPSPSKEEEGLRAQVRDLEEKLETLRLKRSEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPETFDFKIKFAETKAHAKAIEMELRQMEVAQANRHMSLLTAFMPDSFLRPGGDHDCVLVLLLMPRLICKAELIRKQAQEKFDLSENCSERPGLRGAAGEQLSFAAGLVYSLSLLQATLHRYEHALSQCSVDVYKKVGSLYPEMSAHERSLDFLIELLHKDQLDETVNVEPLTKAIKYYQHLYSIHLAEQPEDSTMQLADHIKFTQSALDCMGVEVGRLRAFLQGGQEATDIALLLRDLETSCSDTRQFCKKIRRRMPGTDAPGIPAALAFGSQVSDTLLDCRKHLTWVVAVLQEVAAAAAQLIAPLAENEGLPVAALEELAFKASEQIYGSPSSSPYECLRQSCTILISTMNKLATAMQEGEYDAERPPSKPPPVELRAAALRAEITDAEGLGLKLEDRETVIKELKKSLKIKGEELSEANVRLSLLEKKLDSAAKDADERIEKVQTRLDETQTLLRKKEKDFEETMDALQADIDQLEAEKAELKQRLNSQSKRTIEGLRGPPPSGIATLVSGIAGEEPQRGGAPGQAPGALPGPGLVKDSPLLLQQISAMRLHISQLQHENSILRGAQMKASLAALPPLHVAKLSLPPHEGPGGNLVAGALYRKTSQLLEKLNQLSTHTHVVDITRSSPAAKSPSAQLMEQVAQLKSLSDTIEKLKDEVLKETVTQRPGATVPTDFATFPSSAFLRAKEEQQDDTVYMGKVTFSCAAGLGQRHRLVLTQEQLHQLHSRLIS sun2_mouse SUN domain-containing protein 2 Sad1/unc-84 protein-like 2 Sun2 Unc84b Protein unc-84 homolog B protein protein mouse Mus musculus Mouse MSRRSQRLTRYSQDDNDGGSSSSGASSVAGSQGTVFKDSPLRTLKRKSSNMKHLSPAPQLGPSSDSHTSYYSESVVRESYIGSPRAVSLARSALLDDHLHSEPYWSGDLRGRRRRGTGGSESSKANGLTAESKASEDFFGSSSGYSSEDDLAGYTDSDQHSSGSRLRSAASRAGSFVWTLVTFPGRLFGLLYWWIGTTWYRLTTAASLLDVFVLTRSRHFSLNLKSFLWFLLLLLLLTGLTYGAWHFYPLGLQTLQPAVVSWWAAKESRKQPEVWESRDASQHFQAEQRVLSRVHSLERRLEALAADFSSNWQKEAIRLERLELRQGAAGHGGGSSLSHEDALSLLEGLVSRREATLKEDLRRDTVAHIQEELATLRAEHHQDSEDLFKKIVQASQESEARVQQLKTEWKSMTQEAFQESSVKELGRLEAQLASLRQELAALTLKQNSVADEVGLLPQKIQAARADVESQFPDWIRQFLLGDRGARSGLLQRDEMHAQLQELENKILTKMAEMQGKSAREAAASLGQILQKEGIVGVTEEQVHRIVKQALQRYSEDRIGMVDYALESGGASVISTRCSETYETKTALLSLFGIPLWYHSQSPRVILQPDVHPGNCWAFQGPQGFAVVRLSARIRPTAVTLEHVPKALSPNSTISSAPKDFAIFGFDEDLQQEGTLLGTFAYDQDGEPIQTFYFQASKMATYQVVELRILTNWGHPEYTCIYRFRVHGEPAH sgo2_mouse Shugoshin 2 Sgo2 Sgol2 Sgol2a Shugoshin-2 Shugoshin-like 2 protein protein mouse Mus musculus Mouse MEYPGIKVDTVTSGIQRRVKGRIAKTNLNVSLASKIKAKILNNSSIFKISLKHNNRALARALSKEKENSRRITTEKMQLQKEVEKLNFENTFLRLKLNTLNKKLVEIESHVSNDLLTAIEISSLSEFHQGSFLLSATKKQRNSKQCKPAHLPYARVLLTSENDDDDGADDKWQTKCNNRTISKTSPDSTSSVSRQPSSLHQCNLKAFPPKEDNQKTCGSGHLEHTSSVDILPNESHSDQSPKSSLSEMKTAPSPSLRREKLSHGNVTMRKKCVSSTPDILYVTDLDHQPTSSPGSNWNNEIHGHTNETSNNTQRNAECFLDLPSESSSEPDAKRMELVQKNTDSFHFQKTVYDAADMELTATDIGKIVAVSKSKKNQNKKKADCRKETFRKVKGASSDKKRESSKRECKDGSEVGAEEEADAARAERGAGVLDGRGDSEEPNCISSTEQPSQVNTQKKRTLQNSSDQENIQNTKRRQTYTTDEQEETNPFSRHSVKFLQDGKFDLCQKTLHHNLSKPSRQTFVIRKSEKDNLFPNQEDKDTISENLEVTNEFHIDDLSIEANENVCDHETQTMLDLKKSVSAQQNQTKINKTKQKINRRTKIISVMSQVYEDNDKDIHVLEKDNFPFHTQANKETTSGNLESSKEFESPLLFTRDNGSLRDCKTQNVLDLHKQIPDLYPDRNESQISKIPRQKVNRKTEVISGVKCFSNDQGVHCSEKDKSLLLQKDKDFPGTLKDLSEFDTPAFCNKDSAKSCDYKSEMLLGLKKHDPNMQPACQDDSKAGKKLRQKVNRKTEIISKITQIHENDRGSTHDSLNKKLCQKVNISKIISQMNQIYETINEDGNGFKSSIKDCEDIKSCDFGEINSNKKENYDPIQDPCTLVKKTKRKGSCKAGSSLAGAKNRCGLQLTDSSQVQSVPLDSGLRHHPNEADSGPGEQTNLPKMQKQSAGRSLGDAFSVSLGKEGSRPAKAVSKMTPKSKKRKLPLGCSPETHGTVEITPNTDLAKAVDSQQTEKENYLEKEKIAKRKPDFCTKVLKPLSETCSSNIKNSSLDSMCKSSLPLSISSRKTLMLEESSSLESTCIFQVGDAAHEKITTGTRNPHHRTQKSTPGSRTSLVLVDTSSVSDTNPANPENESEGQSSHPMRRKRQCVPLNLTEPSLRSKMRR sun1_human SUN domain-containing protein 1 KIAA0810 UNC84A Protein unc-84 homolog A Sad1/unc-84 protein-like 1 SUN1 protein protein human Homo sapiens Human MDFSRLHMYSPPQCVPENTGYTYALSSSYSSDALDFETEHKLDPVFDSPRMSRRSLRLATTACTLGDGEAVGADSGTSSAVSLKNRAARTTKQRRSTNKSAFSINHVSRQVTSSGVSHGGTVSLQDAVTRRPPVLDESWIREQTTVDHFWGLDDDGDLKGGNKAAIQGNGDVGAAAATAHNGFSCSNCSMLSERKDVLTAHPAAPGPVSRVYSRDRNQKCYFLLQILRRIGAVGQAVSRTAWSALWLAVVAPGKAASGVFWWLGIGWYQFVTLISWLNVFLLTRCLRNICKFLVLLIPLFLLLAGLSLRGQGNFFSFLPVLNWASMHRTQRVDDPQDVFKPTTSRLKQPLQGDSEAFPWHWMSGVEQQVASLSGQCHHHGENLRELTTLLQKLQARVDQMEGGAAGPSASVRDAVGQPPRETDFMAFHQEHEVRMSHLEDILGKLREKSEAIQKELEQTKQKTISAVGEQLLPTVEHLQLELDQLKSELSSWRHVKTGCETVDAVQERVDVQVREMVKLLFSEDQQGGSLEQLLQRFSSQFVSKGDLQTMLRDLQLQILRNVTHHVSVTKQLPTSEAVVSAVSEAGASGITEAQARAIVNSALKLYSQDKTGMVDFALESGGGSILSTRCSETYETKTALMSLFGIPLWYFSQSPRVVIQPDIYPGNCWAFKGSQGYLVVRLSMMIHPAAFTLEHIPKTLSPTGNISSAPKDFAVYGLENEYQEEGQLLGQFTYDQDGESLQMFQALKRPDDTAFQIVELRIFSNWGHPEYTCLYRFRVHGEPVK sun1-ccdc155-1 1929498 N/A 1929503 unspecified role unspecified role bait bait N/A 1929504 unspecified role unspecified role prey prey physical association physical association false false false 3A sun1-ccdc155-2 1929498 N/A 1929503 unspecified role unspecified role prey prey N/A 1929504 unspecified role unspecified role bait bait N/A 1929505 unspecified role unspecified role prey prey association association false false false 5A ccdc155-sun1-1 1929499 N/A 1929504 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q80VJ8:p.Pro645_Pro647delinsAlaAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false N/A 1929503 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1929521 1929518 physical association physical association false false false 3B ccdc155-sun2-1 1929499 N/A 1929504 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q80VJ8:p.Pro645_Pro647delinsAlaAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false N/A 1929506 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1929527 1929524 physical association physical association false false false 3B sun1-ccdc155-3 1929500 N/A 1929503 unspecified role unspecified role neutral component neutral component N/A 1929504 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2C sgo2-ccdc155-1 1929501 N/A 1929507 unspecified role unspecified role bait bait N/A 1929504 unspecified role unspecified role prey prey physical association physical association false false false no figure ccdc155-sun1-2 1929502 N/A 1929508 unspecified role unspecified role neutral component neutral component N/A 1929504 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q80VJ8:p.Pro645_Pro647delinsAlaAlaAla mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false Q80VJ8:p.Pro645_Pro647del mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false supplemental S3B and S3A