UniProt UniProt http://www.uniprot.org azhibekov-2011-2 mouse-podocyte Mus musculus podocyte renal glomerular podocyte confocal microscopy confocal microscopy immunostaining immunostaining Azhibekov TA., Wu Z., Padiyar A., Bruggeman LA., Simske JS. Experiments using transfected myc-ADAP (fig 1C) omitted as species for FYB/ADAP cannot be established. Host species for experiments with podocytes according ref 24 (PMID 16204413) jsimske@metrohealth.org American journal of physiology. Cell physiology 2011 imex curation Only protein-protein interactions azhibekov-2011-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Azhibekov TA., Wu Z., Padiyar A., Bruggeman LA., Simske JS. Experiments using transfected myc-ADAP (fig 1C) omitted as species for FYB/ADAP cannot be established. Host species for experiments with podocytes according ref 24 (PMID 16204413) jsimske@metrohealth.org 8- to 12.day-old mouse kidney cDNA American journal of physiology. Cell physiology 2011 imex curation Only protein-protein interactions azhibekov-2011-3 mouse-podocyte Mus musculus podocyte renal glomerular podocyte anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Azhibekov TA., Wu Z., Padiyar A., Bruggeman LA., Simske JS. Experiments using transfected myc-ADAP (fig 1C) omitted as species for FYB/ADAP cannot be established. Host species for experiments with podocytes according ref 24 (PMID 16204413) jsimske@metrohealth.org 8- to 12.day-old mouse kidney cDNA American journal of physiology. Cell physiology 2011 imex curation Only protein-protein interactions azhibekov-2011-4 mouse-podocyte Mus musculus podocyte renal glomerular podocyte anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Azhibekov TA., Wu Z., Padiyar A., Bruggeman LA., Simske JS. Experiments using transfected myc-ADAP (fig 1C) omitted as species for FYB/ADAP cannot be established. Host species for experiments with podocytes according ref 24 (PMID 16204413) jsimske@metrohealth.org American journal of physiology. Cell physiology 2011 imex curation Only protein-protein interactions fyb1_mouse FYN-binding protein 1 Fyb1 Adhesion and degranulation promoting adaptor protein FYB-120/130 FYN-T-binding protein Fyb SLAP-130 SLP-76-associated phosphoprotein protein protein mouse Mus musculus Mouse MAKFNTGSNPTEEAATSSRPFKVAGQSSPSGIQSRKNLFDNQGNASPPAGPSSMPKFGTTKPPLAAKPTYEEKPEKEPKPPFLKPTGGSPRFGTQPNSVSRDPEVKVGFLKPVSPKPTSLTKEDSKPVVLRPPGNKLHNLNQESDLKTPGPKPGPAPPVPENELKPGFSKVAGAKSKFMPAAQDTDSKPRFPRHTFGQKPSLSTEDSQEENTSKNVPVQKGSPVQLGAKSKGAPFKPPKEDPEDKDHGAPSSPFPGVVLKPAASRGSPGLSKNFEEKKEDRKTDLAKNIFLNKLNQEEPARFPKAPSKLTAGTPWGQSQEKEGDKNSATPKQKALPPLSVLGPPPPKPNRPPNVDLTRFRKADSANSATKSQTPYSTTSLPPPPPTHPASQPPLPASHPAHPPVPSLPPRNIKPPLDLKHPINDENQDGVMHSDGTGNLEEEQESEGETYEDIDSSKERDKKREKEEKKRLELERKEQKEREKKEQELKKKFKLTGPIQVIHHAKACCDVKGGKNELSFKQGEDIEIIRITDNPEGKWLGRTARGSYGYIKTTAVEIDYDSLKRKKNSLNAVPPRLVEDDQDVYDDVAEQDAPNSHGQSGSGGMFPPPPTDDEIYDGIEEEDDDDGSVPQVDEKTNAWSWGILKMLKGKDDRKKSIREKPKVSESDNNEGSSLPSQHKQLDVGEEVYDDVDASDFPPPPAEMSQGMSVGRAKTEEKDPKKLKKQEKEEKDLRKKFKYDGEIRVLYSTKVASSLTSKKWGARDLQIKPGESLEVIQSTDDTKVLCRNEEGKYGYVLRSYLVDNDGEIYDDIADGCIYDND mint Acts as an adapter protein of the FYN and LCP2 signaling cascades in T cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2 tmm47_mouse Transmembrane protein 47 Transmembrane 4 superfamily member 10 MNCb-0941 Tmem47 Tm4sf10 protein protein mouse Mus musculus Mouse MASAGSGMEEVRVSVLTPLKLVGLVCIFLALCLDLGAVLSPAWVTADHQYYLSLWESCRKPANLDIWHCESTLGSDWQIATLALLLGGAAIILIAFLVGLISICVGSRRRFYRPVAVMLFAAVVLQVCSLVLYPIKFIETVSLKIYHEFNWGYGLAWGATIFSFGGAILYCLNPKNYEDYY fyn_mouse Tyrosine-protein kinase Fyn p59-Fyn Fyn Proto-oncogene c-Fyn protein protein mouse Mus musculus Mouse MGCVQCKDKEAAKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSERAAGLCCRLVVPCHKGMPRLTDLSVKTKDVWEIPRESLQLIKRLGNGQFGEVWMGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQGFLEDYFTATEPQYQPGENL nphn_mouse Nephrin Nphn Renal glomerulus-specific cell adhesion receptor Nphs1 protein protein mouse Mus musculus Mouse MGAKEATVRGPGASPVHRTCHLIPLLLAGMLTTGLAQSPVPTSAPRGFWALSENLTVVEGSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPSVILSILVSPKVLQLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDIIFIQGGRTVEDVSSSVNEGSEEKLFFTEAEARVTPQSSDNGQLLVCEGSNPALATPIKASFTMNILFPPGPPVIDWPGLNEGHVRAGENLELPCIARGGNPPATLQWLKNGKPVSIAWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTILGSTSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTLLVKREDNGLSLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQSIRTGTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNLAKFSCKAGQLSASTQLVVQFPPTNLTILANSSALRPGDALNLTCVSISSNPPVNLSLDKEGERLDDVAAKPQSAPFKGSAASRSVFLRVSSRDHGHRVTCRAHSEALRETVSSFYRLNVLYPPEFLGEQVRAVTVVEQGQALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALKDPTEVNVGGSVDIVCTVDANPILPEMFSWERLGEDEEELNLDDMEKMSKGSTGRLRIRQAKLSQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDHPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPLDLQDPRYTEHKYHQGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSVSPHSVGLEWKPGFDGGLPQRFQIRYEALESPGFLYMDVLPAQATTFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQVSITTPGLDQAPEDTDQPLPTEQPPGPPRLPLLPVLFAVGGLLLLSNASCVGGLLWRRRLRRLAEEISEKTEAGSEEDRIRNEYEESQWTGDRDTRSSTVSTAEVDPHYYSMRDFSPQLPPTLEEVSYRQAFTGIEDEDMAFPGHLYDEVERVYGPPGVWGPLYDEVQMDPYDLRWPEVKYEDPRGIYDQVAADMDAGEPGSLPFELRGHLV fyb-tmem47-2 1931556 N/A 1931560 unspecified role unspecified role neutral component neutral component N/A 1931561 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2F fyn-tmem47-1 1931556 N/A 1931562 unspecified role unspecified role neutral component neutral component N/A 1931561 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false 2I nphs1-tmem47-1 1931556 N/A 1931561 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 1931563 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 5D fyb-tmem47-3 1931556 N/A 1931560 unspecified role unspecified role neutral component neutral component N/A 1931561 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false 2C fyb-tmem47-1 1931557 N/A 1931560 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1931561 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1931583 1931581 physical association physical association false false false no figure tmem47-fyb-1 1931558 N/A 1931560 unspecified role unspecified role prey prey N/A 1931561 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 3A fyn-tmem47-2 1931559 N/A 1931562 unspecified role unspecified role bait bait N/A 1931561 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 3A