IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ lin-2011-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lin Z., Jiang L., Yuan C., Jensen JK., Zhang X., Luo Z., Furie BC., Furie B., Andreasen PA., Huang M. According to reference 27- Zhao -2007 and also this publication, the uPa/PLAU sequence was stated to be "Ile-16–Glu-244 in chymotrypsin numbering". Details of the "chymotrypsin numbering" were found in Egelund et al -2001, pmid:11168406. Figure 1 (from pmid:11168406) states this chymotrypsin numbering in brackets and "Glu 244" corresponds to the first of the two C-terminal glutamines (residue 425 in UniProt P00749). Alignment of the sequence given in the PB header file suggests sequence termnates at leu-431 and this longer sequence has been used in the entry. mhuang@fjirsm.ac.cn The Journal of biological chemistry 2011 imex curation Only protein-protein interactions p05121-pro_0000032499 Plasminogen activator inhibitor 1 SERPINE1 Endothelial plasminogen activator inhibitor PAI1 PLANH1 Serpin E1 protein protein human Homo sapiens Human VHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPYGVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAVDQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPDGHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPKFSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVASSSTAVIVSARMAPEEIIMDRPFLFVVRHNPTGTVLFMGQVMEP P05121 24 402 urok_human Urokinase-type plasminogen activator PLAU protein protein human Homo sapiens Human MRALLARLLLCVLVVSDSKGSNELHQVPSNCDCLNGGTCVSNKYFSNIHWCNCPKKFGGQHCEIDKSKTCYEGNGHFYRGKASTDTMGRPCLPWNSATVLQQTYHAHRSDALQLGLGKHNYCRNPDNRRRPWCYVQVGLKPLVQECMVHDCADGKKPSSPPEELKFQCGQKTLRPRFKIIGGEFTTIENQPWFAAIYRRHRGGSVTYVCGGSLISPCWVISATHCFIDYPKKEDYIVYLGRSRLNSNTQGEMKFEVENLILHKDYSADTLAHHNDIALLKIRSKEGRCAQPSRTIQTICLPSMYNDPQFGTSCEITGFGKENSTDYLYPEQLKMTVVKLISHRECQQPHYYGSEVTTKMLCAADPQWKTDSCQGDSGGPLVCSLQGRMTLTGIVSWGRGCALKDKPGVYTRVSHFLPWIRSHTKEENGLAL plau-pro_0000032499-1 2824789 N/A 2824790 unspecified role unspecified role neutral component neutral component P05121-PRO_0000032499:p.Asn150His mutation mutation certain certain sequence position certain certain certain sequence position certain false P05121-PRO_0000032499:p.Lys154Thr mutation mutation certain certain sequence position certain certain certain sequence position certain false P05121-PRO_0000032499:p.Gln319Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false P05121-PRO_0000032499:p.Met354Ile mutation mutation certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 2824791 unspecified role unspecified role neutral component neutral component P00749:p.Cys299Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false P00749:p.Asn322Gln mutation mutation certain certain sequence position certain certain certain sequence position certain false P00749:p.Ser376Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris Stoichiometry: 1.0 direct interaction direct interaction false false false Table 1 2.3A Rwork 0.22 RFree 0.272