UniProt UniProt http://www.uniprot.org lee-2009-9 in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa predetermined predetermined participant predetermined Lee EC., Desai U., Gololobov G., Hong S., Feng X., Yu XC., Gay J., Wilganowski N., Gao C., Du LL., Chen J., Hu Y., Zhao S., Kirkpatrick L., Schneider M., Zambrowicz BP., Landes G., Powell DR., Sonnenburg WK. wsonnenburg@lexpharma.com The Journal of biological chemistry 2009 imex curation Only protein-protein interactions lee-2009-10 in vitro In vitro lipase assay lipase assay predetermined predetermined participant predetermined Lee EC., Desai U., Gololobov G., Hong S., Feng X., Yu XC., Gay J., Wilganowski N., Gao C., Du LL., Chen J., Hu Y., Zhao S., Kirkpatrick L., Schneider M., Zambrowicz BP., Landes G., Powell DR., Sonnenburg WK. wsonnenburg@lexpharma.com The Journal of biological chemistry 2009 imex curation Only protein-protein interactions lipl_human Lipoprotein lipase LPL LIPD Phospholipase A1 protein protein human Homo sapiens Human MESKALLVLTLAVWLQSLTASRGGVAAADQRRDFIDIESKFALRTPEDTAEDTCHLIPGVAESVATCHFNHSSKTFMVIHGWTVTGMYESWVPKLVAALYKREPDSNVIVVDWLSRAQEHYPVSAGYTKLVGQDVARFINWMEEEFNYPLDNVHLLGYSLGAHAAGIAGSLTNKKVNRITGLDPAGPNFEYAEAPSRLSPDDADFVDVLHTFTRGSPGRSIGIQKPVGHVDIYPNGGTFQPGCNIGEAIRVIAERGLGDVDQLVKCSHERSIHLFIDSLLNEENPSKAYRCSSKEAFEKGLCLSCRKNRCNNLGYEINKVRAKRSSKMYLKTRSQMPYKVFHYQVKIHFSGTESETHTNQAFEISLYGTVAESENIPFTLPEVSTNKTYSFLIYTEVDIGELLMLKLKWKSDSYFSWSDWWSSPGFAIQKIRVKAGETQKKVIFCSREKVSHLQKGKAPAVFVKCHDKSLNKKSG angl4_human Angiopoietin-related protein 4 ANGPTL4 ARP4 HFARP PGAR Angiopoietin-like protein 4 Hepatic fibrinogen/angiopoietin-related protein PP1158 PSEC0166 UNQ171/PRO197 protein protein human Homo sapiens Human MSGAPTAGAALMLCAATAVLLSAQGGPVQSKSPRFASWDEMNVLAHGLLQLGQGLREHAERTRSQLSALERRLSACGSACQGTEGSTDLPLAPESRVDPEVLHSLQTQLKAQNSRIQQLFHKVAQQQRHLEKQHLRIQHLQSQFGLLDHKHLDHEVAKPARRKRLPEMAQPVDPAHNVSRLHRLPRDCQELFQVGERQSGLFEIQPQGSPPFLVNCKMTSDGGWTVIQRRHDGSVDFNRPWEAYKAGFGDPHGEFWLGLEKVHSITGDRNSRLAVQLRDWDGNAELLQFSVHLGGEDTAYSLQLTAPVAGQLGATTVPPSGLSVPFSTWDQDHDLRRDKNCAKSLSGGWWFGTCSHSNLNGQYFRSIPQQRQKLKKGIFWKTWRGRYYPLQATTMLIQPMAAEAAS angl3_human Angiopoietin-related protein 3 ANGPTL3 ANGPT5 UNQ153/PRO179 Angiopoietin-like protein 3 Angiopoietin-5 protein protein human Homo sapiens Human MFTIKLLLFIVPLVISSRIDQDNSSFDSLSPEPKSRFAMLDDVKILANGLLQLGHGLKDFVHKTKGQINDIFQKLNIFDQSFYDLSLQTSEIKEEEKELRRTTYKLQVKNEEVKNMSLELNSKLESLLEEKILLQQKVKYLEEQLTNLIQNQPETPEHPEVTSLKTFVEKQDNSIKDLLQTVEDQYKQLNQQHSQIKEIENQLRRTSIQEPTEISLSSKPRAPRTTPFLQLNEIRNVKHDGIPAECTTIYNRGEHTSGMYAIRPSNSQVFHVYCDVISGSPWTLIQHRIDGSQNFNETWENYKYGFGRLDGEFWLGLEKIYSIVKQSNYVLRIELEDWKDNKHYIEYSFYLGNHETNYTLHLVAITGNVPNAIPENKDLVFSTWDHKAKGHFNCPEGYSGGWWWHDECGENNLNGKYNKPRAKSKPERRRGLSWKSQNGRLYSIKSTKMLIHPTDSESFE 1,2-di-o-lauryl-rac-glycero-3-glutaric acid 6'-methylresorufin ester 1,2-di-O-lauryl-rac-glycero-3-glutaric acid 6'-methylresorufin ester 1,2-di-O-dodecyl-rac-glycero-3-glutaric acid 6'-methylresorufin ester DGGR 1-[2,3-bis(dodecyloxy)propyl] 6-methyl-3-oxo-3H-phenoxazin-7-yl pentanedioate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) angptl4-lpl-3 1907020 N/A 1907022 unspecified role unspecified role prey prey c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 1907023 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false antibody aginst this epitope decreases association region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 2, S4e, f angptl3-lpl-2 1907020 N/A 1907024 unspecified role unspecified role bait bait region binding region binding-associated region binding region range range range range false Epitope-specific antibody impairs association region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 1907022 unspecified role unspecified role prey prey c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 6 1,2_di_o_lauryl_rac_glycero_3_glutaric acid 6'_methylresorufin ester-angptl3-1 1907021 N/A 1907025 enzyme target enzyme target substrate substrate unspecified role unspecified role N/A 1907022 enzyme enzyme unspecified role unspecified role c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 1907024 inhibitor inhibitor unspecified role unspecified role c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region required to bind necessary binding region required to bind range range range range false ecoli Escherichia coli (strain K12) 1907021 direct interaction direct interaction false false false 5a and b ANGPTL3 inhibits LPL activity by approximat. 50%. Addition of epitope-specific antibody prevented inhibition by ANGPTL3 with an Ec of 22 nM. 1,2_di_o_lauryl_rac_glycero_3_glutaric acid 6'_methylresorufin ester-lpl-1 1907021 N/A 1907025 enzyme target enzyme target substrate substrate unspecified role unspecified role N/A 1907022 enzyme enzyme unspecified role unspecified role c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 5a