IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ wang-1996-3 xenla-oocyte Xenopus laevis oocyte Oocyte anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang F., Gerzanich V., Wells GB., Anand R., Peng X., Keyser K., Lindstrom J. The Journal of biological chemistry 1996 Only protein-protein interactions imex curation imex curation wang-1996-4 xenla-oocyte Xenopus laevis oocyte Oocyte anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wang F., Gerzanich V., Wells GB., Anand R., Peng X., Keyser K., Lindstrom J. The Journal of biological chemistry 1996 Only protein-protein interactions imex curation imex curation wang-1996-5 human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang F., Gerzanich V., Wells GB., Anand R., Peng X., Keyser K., Lindstrom J. The Journal of biological chemistry 1996 Only protein-protein interactions imex curation imex curation acha5_human Neuronal acetylcholine receptor subunit alpha-5 CHRNA5 NACHRA5 protein protein human Homo sapiens Human MAARGSGPRALRLLLLVQLVAGRCGLAGAAGGAQRGLSEPSSIAKHEDSLLKDLFQDYERWVRPVEHLNDKIKIKFGLAISQLVDVDEKNQLMTTNVWLKQEWIDVKLRWNPDDYGGIKVIRVPSDSVWTPDIVLFDNADGRFEGTSTKTVIRYNGTVTWTPPANYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDIILEDQDVDKRDFFDNGEWEIVSATGSKGNRTDSCCWYPYVTYSFVIKRLPLFYTLFLIIPCIGLSFLTVLVFYLPSNEGEKICLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIMVTVFAINIHHRSSSTHNAMAPLVRKIFLHTLPKLLCMRSHVDRYFTQKEETESGSGPKSSRNTLEAALDSIRYITRHIMKENDVREVVEDWKFIAQVLDRMFLWTFLFVSIVGSLGLFVPVIYKWANILIPVHIGNANK achb2_human Neuronal acetylcholine receptor subunit beta-2 CHRNB2 protein protein human Homo sapiens Human MARRCGPVALLLGFGLLRLCSGVWGTDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISVHEREQIMTTNVWLTQEWEDYRLTWKPEEFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDLVLKSEVASLDDFTPSGEWDIVALPGRRNENPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMAPWVKVVFLEKLPALLFMQQPRHHCARQRLRLRRRQREREGAGALFFREAPGADSCTCFVNRASVQGLAGAFGAEPAPVAGPGRSGEPCGCGLREAVDGVRFIADHMRSEDDDQSVSEDWKYVAMVIDRLFLWIFVFVCVFGTIGMFLQPLFQNYTTTTFLHSDHSAPSSK acha3_human Neuronal acetylcholine receptor subunit alpha-3 CHRNA3 NACHRA3 protein protein human Homo sapiens Human MGSGPLSLPLALSPPRLLLLLLLSLLPVARASEAEHRLFERLFEDYNEIIRPVANVSDPVIIHFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWNPSDYGGAEFMRVPAQKIWKPDIVLYNNAVGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAIIKAPGYKHDIKYNCCEEIYPDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPSWVKTVFLNLLPRVMFMTRPTSNEGNAQKPRPLYGAELSNLNCFSRAESKGCKEGYPCQDGMCGYCHHRRIKISNFSANLTRSSSSESVDAVLSLSALSPEIKEAIQSVKYIAENMKAQNEAKEIQDDWKYVAMVIDRIFLWVFTLVCILGTAGLFLQPLMAREDA chrnb2-chrna5-1 1790323 N/A 1790326 unspecified role unspecified role prey prey tag epitope SQVTGEVIFQTPLIKNPLQQVTGEVIFQTPLIKNPLQ tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 1790327 unspecified role unspecified role bait bait physical association physical association false false false Fig. 7a (lanes 5&6) chrna5-chrna3-1 1790324 N/A 1790326 unspecified role unspecified role bait bait tag epitope SQVTGEVIFQTPLIKNPLQQVTGEVIFQTPLIKNPLQ tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 1790328 unspecified role unspecified role prey prey N/A 1790327 unspecified role unspecified role prey prey association association false false false Fig. 7a (lanes 9, 12 & 14) beta2 is required for this interaction as without it, alpha3 is not detected (lane 10). chrnb2-chrna3-1 1790325 N/A 1790326 unspecified role unspecified role prey prey N/A 1790328 unspecified role unspecified role prey prey N/A 1790327 unspecified role unspecified role bait bait association association false false false Fig 7b (lanes 2 & 3)