IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ conroy-1995-3 chick-neuron Chick neuron neuron Neuron [Neuronal cell; Neural cell solid phase assay solid phase assay antibody detection identification by antibody antibody detection Conroy WG., Berg DK. Tissue used is ciliary ganglion extract from chicks. In Fig. 7, extracts were immunodepleted by beta2 or alpha3 antibodies prior to the solid phase assay and preys identified by gamma counting of the 125[I]mAb35 against alpha5 subunit. dberg@ucsd.edu The Journal of biological chemistry 1995 Only protein-protein interactions imex curation imex curation Conroy WG., Berg DK., Conroy WG., Berg DK. conroy-1995-1 chick-neuron Chick neuron neuron Neuron [Neuronal cell; Neural cell density sedimentation cosedimentation through density gradient density sedimentation antibody detection identification by antibody antibody detection Conroy WG., Berg DK. Tissue used is ciliary ganglion extract from chicks. In Fig. 1, extracts were immunodepleted prior to fractionation on a sucrose gradient, IPed using solid phase immunoprecipitation and preys identified by gamma counting of the 125[I]mAb35 against alpha5 subunit. dberg@ucsd.edu The Journal of biological chemistry 1995 Only protein-protein interactions imex curation imex curation Conroy WG., Berg DK., Conroy WG., Berg DK. conroy-1995-2 chick-neuron Chick neuron neuron Neuron [Neuronal cell; Neural cell anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Conroy WG., Berg DK. Tissue used is ciliary ganglion extract from chicks. dberg@ucsd.edu The Journal of biological chemistry 1995 Only protein-protein interactions imex curation imex curation Conroy WG., Berg DK., Conroy WG., Berg DK. conroy-1995-4 chick-neuron Chick neuron neuron Neuron [Neuronal cell; Neural cell solid phase assay solid phase assay tag visualisation tag visualisation Conroy WG., Berg DK., Conroy WG., Berg DK. Tissue used is ciliary ganglion extract from chicks. dberg@ucsd.edu The Journal of biological chemistry 1995 Only protein-protein interactions imex curation imex curation acha5_chick Neuronal acetylcholine receptor subunit alpha-5 CHRNA5 protein protein chick Gallus gallus (Chicken) Chicken MPLRARSRKPGAGPAARAPQAGVSEPSFVAKSEDRLFKHLFEDYQRWVRPVEHLNDTIKIKFGLAISQLVDVDEKNQLMTTNVWLKQEWIHVKLRWNPEDYAGITSIRVPSDSIWIPDIVLYDNADGRFEGTSTKTVVKYDGTIAWTPPVNYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDIILEDYEVDKRDFFDNGEWEIVTATGSKGNRTDGCCWYPFVTYSFIIRRLPLFYTLFLIIPCIGLSFLTVLVFYLPSNEAEKISLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIVITVFAINIHHRSSSTHNAMAPWVRKIFLHKLPKLLCMRSHVDRYFAQKEEKGNMSGSESSRNTLEAALDSIRYITRHVMKENEVREVVEDWKFIAQVLDRMFLWAFLLVSIIGSLVLFIPVIHKWASIIVPVHIGSTNT achb4_chick Neuronal acetylcholine receptor subunit beta-4 CHRNB4 Neuronal acetylcholine receptor non-alpha-3 chain protein protein chick Gallus gallus (Chicken) Chicken STAADAEEKLMNHLLSPDRYNKLIRPAVNSSQLVSIELQVSLAQLISVNEREQIMTTNVWLNQEWIDYRLAWKPSDYEGINMLRIPAKHIWLPDIVLYNNADGTYEVSLYTNAIVQNNGSIRWLPPAIYKSACKIEVKHFPFDQQNCTLKFRSWTYDHTEIDMVLKTSMASMDDFTPSGEWDIVALPGRRTENPLDPNYVDVTYDFIIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLIGKYLMFTMVLVTFSIVTSVCVLNVHHRSPSTHTMPPWVKLVFLERLPAYLFMKRPENNSPRQKPANCKKTRAENLCMDPADFYKNSTYFVNTASAKKYDMKITDTLDNVSSHQDFRLRTGTKFSPEVQEAIDGVSFIAEHMKSDDNDQSVIEDWKYVAMVVDRLFLWIFVLVCVLGTVGLFLQPLFQNHIAATNP achb2_chick Neuronal acetylcholine receptor subunit beta-2 CHRNB2 Neuronal acetylcholine receptor non-alpha-1 chain protein protein chick Gallus gallus (Chicken) Chicken MALLRVLCLLAALRRSLCTDTEERLVEYLLDPTRYNKLIRPATNGSQLVTVQLMVSLAQLISVHEREQIMTTNVWLTQEWEDYRLTWKPEDFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVISYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDLVLKSEVASLDDFTPSGEWDIVALPGRRNENPDDSTYVDITYDFIIRRKPLFYTINLIIPCILITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMPPWVRTLFLRKLPALLFMKQPQQNCARQRLRQRRQTQERAAAATLFLRAGARACACYANPGAAKAEGLNGYRERQGQGPDPPAPCGCGLEEAVEGVRFIADHMRSEDDDQSVSEDWKYVAMVIDRLFLWIFVFVCVFGTVGMFLQPLFQNYATNSLLQLGQGTPTSK acha3_chick Neuronal acetylcholine receptor subunit alpha-3 CHRNA3 protein protein chick Gallus gallus (Chicken) Chicken MQSIHALLLTAAVCILFQGCGGSEPEHRLYAALFKNYNQFVRPVKNASDPVIIQFEVSMSQLVKVDEVNQIMETNLWLKHIWNDYKLRWNPVDYGGAEFIRVPSGQIWKPDIVLYNNAVGDFQVDDKTKALLKYTGDVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSTMNLKDYWESGEWAIIKAPGYKHDIKYNCCEEIYTDITYSLYIRRLPLFYTINMIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPKTHTMPVWVRTIFLNLLPRIMFMTRPTSDEENNQKPKPFYTSEFSNLNCFNSSEIKCCKDGFVCQDMACSCCQYQRMKFSDFSGNLTRSSSSESVDPLFSFSVLSPEMRDAIESVKYIAENMKMQNEAKEIQDDWKYVAMVIDRIFLWVFILVCILGTAGLFLQPLMTGDDM 3l21a_bunmu Alpha-bungarotoxin Long neurotoxin 1 Alpha-bungarotoxin, isoform A31 Alpha-elapitoxin-Bm2a protein protein bunmu Bungarus multicinctus Many-banded krait MKTLLLTLVVVTIVCLDLGYTIVCHTTATSPISAVTCPPGENLCYRKMWCDAFCSSRGKVVELGCAATCPSKKPYEEVTCCSTDKCNPHPKQRPG mint Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Binds to muscular and neuronal (alpha-7, alpha-8, and alpha-9) nicotinic acetylcholine receptors. Blocks the extracellular increase of dopamine evoked by nicotine only at the higher dose (4.2 uM) acha7_chick Neuronal acetylcholine receptor subunit alpha-7 CHRNA7 protein protein chick Gallus gallus (Chicken) Chicken MGLRALMLWLLAAAGLVRESLQGEFQRKLYKELLKNYNPLERPVANDSQPLTVYFTLSLMQIMDVDEKNQVLTTNIWLQMYWTDHYLQWNVSEYPGVKNVRFPDGLIWKPDILLYNSADERFDATFHTNVLVNSSGHCQYLPPGIFKSSCYIDVRWFPFDVQKCNLKFGSWTYGGWSLDLQMQEADISGYISNGEWDLVGIPGKRTESFYECCKEPYPDITFTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLQYHHHDPDGGKMPKWTRVILLNWCAWFLRMKRPGEDKVRPACQHKQRRCSLSSMEMNTVSGQQCSNGNMLYIGFRGLDGVHCTPTTDSGVICGRMTCSPTEEENLLHSGHPSEGDPDLAKILEEVRYIANRFRDQDEEEAICNEWKFAASVVDRLCLMAFSVFTIICTIGILMSAPNFVEAVSKDFA chrnb4-chrna5-2 1799411 N/A 1799415 unspecified role unspecified role prey prey N/A 1799416 unspecified role unspecified role bait bait physical association physical association false false false Fig. 6a chrnb2-chrna5-2 1799411 N/A 1799415 unspecified role unspecified role prey prey N/A 1799417 unspecified role unspecified role bait bait physical association physical association false false false Fig. 6a, 7 chrnb2-chrna5-4 1799411 N/A 1799418 unspecified role unspecified role prey prey N/A 1799415 unspecified role unspecified role prey prey N/A 1799417 unspecified role unspecified role bait bait physical association physical association false false false Fig. 7 chrnb4-chrna5-3 1799411 N/A 1799418 unspecified role unspecified role prey prey N/A 1799415 unspecified role unspecified role prey prey N/A 1799416 unspecified role unspecified role bait bait physical association physical association false false false Fig. 7 chrna3-chrna5-1 1799412 N/A 1799418 unspecified role unspecified role neutral component neutral component N/A 1799415 unspecified role unspecified role neutral component neutral component N/A 1799416 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 1 Alpha3 and beta4 subunits act as baits. chrnb4-chrna5-1 1799412 N/A 1799416 unspecified role unspecified role bait bait N/A 1799415 unspecified role unspecified role prey prey physical association physical association false false false Fig. 5 chrnb2-chrna5-5 1799412 N/A 1799417 unspecified role unspecified role bait bait N/A 1799415 unspecified role unspecified role prey prey physical association physical association false false false Fig. 5 chrnb2-chrna3-2 1799413 N/A 1799418 unspecified role unspecified role prey prey N/A 1799417 unspecified role unspecified role bait bait N/A 1799415 unspecified role unspecified role prey prey N/A 1799416 unspecified role unspecified role prey prey association association false false false Fig. 3b chrnb2-chrna3-3 1799413 N/A 1799418 unspecified role unspecified role prey prey N/A 1799417 unspecified role unspecified role prey prey N/A 1799415 unspecified role unspecified role bait bait N/A 1799416 unspecified role unspecified role prey prey association association false false false Fig. 4 chrna7-3l21a_bunmu-1 1799414 N/A 1799419 unspecified role unspecified role prey prey 125i radiolabel 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1799420 unspecified role unspecified role bait bait physical association physical association false false false Fig. 6b