IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ wang-2015-10 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang L., Yu Y., Chow DC., Yan F., Hsu CC., Stossi F., Mancini MA., Palzkill T., Liao L., Zhou S., Xu J., Lonard DM., O'Malley BW. Results from fig 4E not added as the specificity of the Abl antibody is not known. GST-Src3 (and Flag-SRC-3) were determined to be of Human origin by following through references to PMID:15383283 and also by mutation mapping in Wu et al., 2007 (PMID:17574025). Mutation mapping indicates SRC3 isoform 1 was used, and Figure 5 of PMID:17574025 gives the "RID" (Receptor Interacting Domain) as amino acids 582-840. However the full length protein is stated to be 1417 amino acids in figure 5;this length does not correspond to any known SRC3 protein. dlonard@bcm.edu,berto@bcm.edu Cancer cell 2015 Only protein-protein interactions imex curation imex curation wang-2015-9 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Wang L., Yu Y., Chow DC., Yan F., Hsu CC., Stossi F., Mancini MA., Palzkill T., Liao L., Zhou S., Xu J., Lonard DM., O'Malley BW. GST-Src3 (and Flag-SRC-3) were determined to be of Human origin by following through references to PMID:15383283 and also by mutation mapping in Wu et al., 2007 (PMID:17574025). Mutation mapping indicates SRC3 isoform 1 was used, and Figure 5 of PMID:17574025 gives the "RID" (Receptor Interacting Domain) as amino acids 582-840. However the full length protein is stated to be 1417 amino acids in figure 5;this length does not correspond to any known SRC3 protein. dlonard@bcm.edu,berto@bcm.edu Cancer cell 2015 Only protein-protein interactions imex curation imex curation wang-2015-11 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Wang L., Yu Y., Chow DC., Yan F., Hsu CC., Stossi F., Mancini MA., Palzkill T., Liao L., Zhou S., Xu J., Lonard DM., O'Malley BW. GST-Src3 (and Flag-SRC-3) were determined to be of Human origin by following through references to PMID:15383283 and also by mutation mapping in Wu et al., 2007 (PMID:17574025). Mutation mapping indicates SRC3 isoform 1 was used, and Figure 5 of PMID:17574025 gives the "RID" (Receptor Interacting Domain) as amino acids 582-840. However the full length protein is stated to be 1417 amino acids in figure 5;this length does not correspond to any known SRC3 protein. dlonard@bcm.edu,berto@bcm.edu Cancer cell 2015 Only protein-protein interactions imex curation imex curation cbp_human CREB-binding protein CREBBP CBP Histone lysine acetyltransferase CREBBP Protein-lysine acetyltransferase CREBBP protein protein human Homo sapiens Human MAENLLDGPPNPKRAKLSSPGFSANDSTDFGSLFDLENDLPDELIPNGGELGLLNSGNLVPDAASKHKQLSELLRGGSGSSINPGIGNVSASSPVQQGLGGQAQGQPNSANMASLSAMGKSPLSQGDSSAPSLPKQAASTSGPTPAASQALNPQAQKQVGLATSSPATSQTGPGICMNANFNQTHPGLLNSNSGHSLINQASQGQAQVMNGSLGAAGRGRGAGMPYPTPAMQGASSSVLAETLTQVSPQMTGHAGLNTAQAGGMAKMGITGNTSPFGQPFSQAGGQPMGATGVNPQLASKQSMVNSLPTFPTDIKNTSVTNVPNMSQMQTSVGIVPTQAIATGPTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRACSLPHCRTMKNVLNHMTHCQAGKACQVAHCASSRQIISHWKNCTRHDCPVCLPLKNASDKRNQQTILGSPASGIQNTIGSVGTGQQNATSLSNPNPIDPSSMQRAYAALGLPYMNQPQTQLQPQVPGQQPAQPQTHQQMRTLNPLGNNPMNIPAGGITTDQQPPNLISESALPTSLGATNPLMNDGSNSGNIGTLSTIPTAAPPSSTGVRKGWHEHVTQDLRSHLVHKLVQAIFPTPDPAALKDRRMENLVAYAKKVEGDMYESANSRDEYYHLLAEKIYKIQKELEEKRRSRLHKQGILGNQPALPAPGAQPPVIPQAQPVRPPNGPLSLPVNRMQVSQGMNSFNPMSLGNVQLPQAPMGPRAASPMNHSVQMNSMGSVPGMAISPSRMPQPPNMMGAHTNNMMAQAPAQSQFLPQNQFPSSSGAMSVGMGQPPAQTGVSQGQVPGAALPNPLNMLGPQASQLPCPPVTQSPLHPTPPPASTAAGMPSLQHTTPPGMTPPQPAAPTQPSTPVSSSGQTPTPTPGSVPSATQTQSTPTVQAAAQAQVTPQPQTPVQPPSVATPQSSQQQPTPVHAQPPGTPLSQAAASIDNRVPTPSSVASAETNSQQPGPDVPVLEMKTETQAEDTEPDPGESKGEPRSEMMEEDLQGASQVKEETDIAEQKSEPMEVDEKKPEVKVEVKEEEESSSNGTASQSTSPSQPRKKIFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDIVKNPMDLSTIKRKLDTGQYQEPWQYVDDVWLMFNNAWLYNRKTSRVYKFCSKLAEVFEQEIDPVMQSLGYCCGRKYEFSPQTLCCYGKQLCTIPRDAAYYSYQNRYHFCEKCFTEIQGENVTLGDDPSQPQTTISKDQFEKKKNDTLDPEPFVDCKECGRKMHQICVLHYDIIWPSGFVCDNCLKKTGRPRKENKFSAKRLQTTRLGNHLEDRVNKFLRRQNHPEAGEVFVRVVASSDKTVEVKPGMKSRFVDSGEMSESFPYRTKALFAFEEIDGVDVCFFGMHVQEYGSDCPPPNTRRVYISYLDSIHFFRPRCLRTAVYHEILIGYLEYVKKLGYVTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQEWYKKMLDKAFAERIIHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLEESIKELEQEEEERKKEESTAASETTEGSQGDSKNAKKKNNKKTNKNKSSISRANKKKPSMPNVSNDLSQKLYATMEKHKEVFFVIHLHAGPVINTLPPIVDPDPLLSCDLMDGRDAFLTLARDKHWEFSSLRRSKWSTLCMLVELHTQGQDRFVYTCNECKHHVETRWHCTVCEDYDLCINCYNTKSHAHKMVKWGLGLDDEGSSQGEPQSKSPQESRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRVVQHTKGCKRKTNGGCPVCKQLIALCCYHAKHCQENKCPVPFCLNIKHKLRQQQIQHRLQQAQLMRRRMATMNTRNVPQQSLPSPTSAPPGTPTQQPSTPQTPQPPAQPQPSPVSMSPAGFPSVARTQPPTTVSTGKPTSQVPAPPPPAQPPPAAVEAARQIEREAQQQQHLYRVNINNSMPPGRTGMGTPGSQMAPVSLNVPRPNQVSGPVMPSMPPGQWQQAPLPQQQPMPGLPRPVISMQAQAAVAGPRMPSVQPPRSISPSALQDLLRTLKSPSSPQQQQQVLNILKSNPQLMAAFIKQRTAKYVANQPGMQPQPGLQSQPGMQPQPGMHQQPSLQNLNAMQAGVPRPGVPPQQQAMGGLNPQGQALNIMNPGHNPNMASMNPQYREMLRRQLLQQQQQQQQQQQQQQQQQQGSAGMAGGMAGHGQFQQPQGPGGYPPAMQQQQRMQQHLPLQGSSMGQMAAQMGQLGQMGQPGLGADSTPNIQQALQQRILQQQQMKQQIGSPGQPNPMSPQQHMLSGQPQASHLPGQQIATSLSNQVRSPAPVQSPRPQSQPPHSSPSPRIQPQPSPHHVSPQTGSPHPGLAVTMASSIDQGHLGNPEQSAMLPQLNTPSRSALSSELSLVGDTTGDTLEKFVEGL carm1_human Histone-arginine methyltransferase CARM1 CARM1 PRMT4 Protein arginine N-methyltransferase 4 Coactivator-associated arginine methyltransferase 1 protein protein human Homo sapiens Human MAAAAAAVGPGAGGAGSAVPGGAGPCATVSVFPGARLLTIGDANGEIQRHAEQQALRLEVRAGPDSAGIALYSHEDVCVFKCSVSRETECSRVGKQSFIITLGCNSVLIQFATPNDFCSFYNILKTCRGHTLERSVFSERTEESSAVQYFQFYGYLSQQQNMMQDYVRTGTYQRAILQNHTDFKDKIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTYNLSSGMAVAGMPTAYDLSSVIASGSSVGHNNLIPLANTGIVNHTHSRMGSIMSTGIVQGSSGAQGSGGGSTSAHYAVNSQFTMGGPAISMASPMSIPTNTMHYGS ncoa3_human Nuclear receptor coactivator 3 NCOA3 AIB1 RAC3 Thyroid hormone receptor activator molecule 1 Receptor-associated coactivator 3 BHLHE42 Steroid receptor coactivator protein 3 CBP-interacting protein Amplified in breast cancer 1 protein ACTR Class E basic helix-loop-helix protein 42 TRAM1 protein protein human Homo sapiens Human MSGLGENLDPLASDSRKRKLPCDTPGQGLTCSGEKRRREQESKYIEELAELISANLSDIDNFNVKPDKCAILKETVRQIRQIKEQGKTISNDDDVQKADVSSTGQGVIDKDSLGPLLLQALDGFLFVVNRDGNIVFVSENVTQYLQYKQEDLVNTSVYNILHEEDRKDFLKNLPKSTVNGVSWTNETQRQKSHTFNCRMLMKTPHDILEDINASPEMRQRYETMQCFALSQPRAMMEEGEDLQSCMICVARRITTGERTFPSNPESFITRHDLSGKVVNIDTNSLRSSMRPGFEDIIRRCIQRFFSLNDGQSWSQKRHYQEAYLNGHAETPVYRFSLADGTIVTAQTKSKLFRNPVTNDRHGFVSTHFLQREQNGYRPNPNPVGQGIRPPMAGCNSSVGGMSMSPNQGLQMPSSRAYGLADPSTTGQMSGARYGGSSNIASLTPGPGMQSPSSYQNNNYGLNMSSPPHGSPGLAPNQQNIMISPRNRGSPKIASHQFSPVAGVHSPMASSGNTGNHSFSSSSLSALQAISEGVGTSLLSTLSSPGPKLDNSPNMNITQPSKVSNQDSKSPLGFYCDQNPVESSMCQSNSRDHLSDKESKESSVEGAENQRGPLESKGHKKLLQLLTCSSDDRGHSSLTNSPLDSSCKESSVSVTSPSGVSSSTSGGVSSTSNMHGSLLQEKHRILHKLLQNGNSPAEVAKITAEATGKDTSSITSCGDGNVVKQEQLSPKKKENNALLRYLLDRDDPSDALSKELQPQVEGVDNKMSQCTSSTIPSSSQEKDPKIKTETSEEGSGDLDNLDAILGDLTSSDFYNNSISSNGSHLGTKQQVFQGTNSLGLKSSQSVQSIRPPYNRAVSLDSPVSVGSSPPVKNISAFPMLPKQPMLGGNPRMMDSQENYGSSMGGPNRNVTVTQTPSSGDWGLPNSKAGRMEPMNSNSMGRPGGDYNTSLPRPALGGSIPTLPLRSNSIPGARPVLQQQQQMLQMRPGEIPMGMGANPYGQAAASNQLGSWPDGMLSMEQVSHGTQNRPLLRNSLDDLVGPPSNLEGQSDERALLDQLHTLLSNTDATGLEEIDRALGIPELVNQGQALEPKQDAFQGQEAAVMMDQKAGLYGQTYPAQGPPMQGGFHLQGQSPSFNSMMNQMNQQGNFPLQGMHPRANIMRPRTNTPKQLRMQLQQRLQGQQFLNQSRQALELKMENPTAGGAAVMRPMMQPQVSSQQGFLNAQMVAQRSRELLSHHFRQQRVAMMMQQQQQQQQQQQQQQQQQQQQQQQQQQQQQTQAFSPPPNVTASPSMDGLLAGPTMPQAPPQQFPYQPNYGMGQQPDPAFGRVSSPPNAMMSSRMGPSQNPMMQHPQAASIYQSSEMKGWPSGNLARNSSFSQQQFAHQGNPAVYSMVHMNGSSGHMGQMNMNPMPMSGMPMGPDQKYC mcb-613 MCB-613 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ncoa3-carm1-1 1886952 N/A 1886955 unspecified role unspecified role prey prey N/A 1886956 unspecified role unspecified role prey prey N/A 1886957 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 1H, Supp 1 G/H-1 MCB-613 - CHEBI:90954 ncoa3-carm1-2 1886952 N/A 1886956 unspecified role unspecified role prey prey N/A 1886957 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1H ncoa3-carm1-3 1886952 N/A 1886955 unspecified role unspecified role prey prey N/A 1886956 unspecified role unspecified role prey prey N/A 1886957 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 1H, Supp 1 G/H-2 MCB-613 - CHEBI:90954 ncoa3-carm1-4 1886952 N/A 1886955 unspecified role unspecified role prey prey N/A 1886956 unspecified role unspecified role prey prey N/A 1886957 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 1H, Supp 1 G/H-3 MCB-613 - CHEBI:90954 ncoa3-carm1-5 1886952 N/A 1886955 unspecified role unspecified role prey prey N/A 1886956 unspecified role unspecified role prey prey N/A 1886957 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 1H, Supp 1 G/H-4 MCB-613 - CHEBI:90954 ncoa3-carm1-6 1886952 N/A 1886955 unspecified role unspecified role prey prey N/A 1886956 unspecified role unspecified role prey prey N/A 1886957 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 1H, Supp 1 G/H-5 MCB-613 - CHEBI:90954 ncoa3-carm1-7 1886952 N/A 1886955 unspecified role unspecified role prey prey N/A 1886956 unspecified role unspecified role prey prey N/A 1886957 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 1H, Supp 1 G/H-6 MCB-613 - CHEBI:90954 ncoa3-carm1-8 1886952 N/A 1886955 unspecified role unspecified role prey prey N/A 1886956 unspecified role unspecified role prey prey N/A 1886957 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 1H, Supp 1 G/H-7 MCB-613 - CHEBI:90954 Longer treatment with MCB-613 for 4 or 8 hr increased the SRC-3-CBP interaction, whereas no further interaction between SRC-3 and CARM1 was observed. ncoa3-carm1-9 1886952 N/A 1886955 unspecified role unspecified role prey prey N/A 1886956 unspecified role unspecified role prey prey N/A 1886957 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 1H, Supp 1 G/H-8 MCB-613 - CHEBI:90954 Longer treatment with MCB-613 for 4 or 8 hr increased the SRC-3-CBP interaction, whereas no further interaction between SRC-3 and CARM1 was observed. ncoa3-carm1-10 1886952 N/A 1886955 unspecified role unspecified role prey prey N/A 1886956 unspecified role unspecified role prey prey N/A 1886957 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 1H, Supp 1 G/H-9 MCB-613 - CHEBI:90954 Longer treatment with MCB-613 for 4 or 8 hr increased the SRC-3-CBP interaction, whereas no further interaction between SRC-3 and CARM1 was observed. ncoa3-carm1-12 1886952 N/A 1886955 unspecified role unspecified role prey prey N/A 1886956 unspecified role unspecified role prey prey N/A 1886957 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 1H, Supp 1 G/H-11 MCB-613 - CHEBI:90954 Longer treatment with MCB-613 for 4 or 8 hr increased the SRC-3-CBP interaction, whereas no further interaction between SRC-3 and CARM1 was observed. ncoa3-carm1-13 1886952 N/A 1886955 unspecified role unspecified role prey prey N/A 1886956 unspecified role unspecified role prey prey N/A 1886957 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 1H, Supp 1 G/H-10 MCB-613 - CHEBI:90954 Longer treatment with MCB-613 for 4 or 8 hr increased the SRC-3-CBP interaction, whereas no further interaction between SRC-3 and CARM1 was observed. ncoa3-mcb_613-1 1886953 N/A 1886957 unspecified role unspecified role bait bait rid_region sufficient to bind sufficient binding region sufficient to bind range range range range false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 1886958 unspecified role unspecified role prey prey direct interaction direct interaction false false false 1G CHEBI:90954 increases the expression at the RNA level of Q9Y6Q9 CHEBI:90954 decreases the expression at the protein level of Q9Y6Q9 CHEBI:28939 decreased the stimulatory effect of CHEBI:90954 on the expression at the RNA level of Q9Y6Q9 CHEBI:28939 reversed the decrease in protein levels of Q9Y6Q9 caused by CHEBI:90954 ncoa3-mcb_613-2 1886954 N/A 1886957 unspecified role unspecified role neutral component neutral component rid_region sufficient to bind sufficient binding region sufficient to bind range range range range false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 1886958 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false Supp 1F