MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint nrskov_lauritsen-2015-1 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. htrf homogeneous time resolved fluorescence homogeneous time-resolved fluorescence htrf tag fluorescence tag visualisation by fluorescence tag fluorescence Nørskov-Lauritsen L., Jørgensen S., Bräuner-Osborne H. hbo@sund.ku.dk FEBS letters 2015 Only protein-protein interactions imex curation imex curation gpc6a_rat G-protein coupled receptor family C group 6 member A Gprc6a protein protein rat Rattus norvegicus (Rat) Rat MALSFVFITCFMILLDTSQSCHTPDDFVAITSPGHIMIGGLFAIHEKMLSSDDHPRQPQIQKCVGFEISVFLQTLAMIHSIEMINNSSLLSGVKLGYEIYDTCTEVTAAMAATLRFLSKFNCSRETVIFQCDYSSYVPRVKAIIGAGYSEISMAVSRMLNLQLMPQVSYESTAEILSDKIRFPSFLRTVPSDFYQTKAMAHLIRQSGWNWVGAITTDDDYGRLALNTFAIQAAENNVCIAFKEVLPAFLSDNTIEVRINQTLEKIIAEAQVNVIVVFLRKFHVFNLFNKAIERKISKIWIASDNWSTAAKIITIPNVKKLGKVVGFTFRRGNMSSFHSFLQTLHMYPSDNNKPLHEFAMLFSACKHIKDGDLSQCISNYSQATWTYDTTKTIETHLFKRNDFLWHYTEPGLIHSIQLAVLALGHAIRDLCQDRDCQKPNAFQPWELLAVLKNVTFTDGKNSFHFDAHGDLNTGYEVVLWKETNGLMTVTKMAEYDLQHDVFITTNQETKHEFRKLKQILSKCSKECIPGQMKKATGSQHSCCYECVNCPENHYSNETDMDHCLVCNNETHWAPVRSTMCFEKEVEYLDWDDSLALLLIALSLLGIAFVLAVGIIFTRNLKTPVVKSSGGLVVCYVMLACHALNFASTGFFIGEPQDFTCKTRQTLFGVSFTLCVSCILTKSLKILLAFSFDPTLKTFLKCLYRPVPIVLTCTGIQVVICTLWLVLAAPTVEENTSLPRVIILECEEGSALAFGTMLGYIAVLAFICFVFAFKGRKLPENYNEAKFLTFGMLIYFIAWITFIPVYATTFGKYLPAVEIIVILISNYGILCCTFFPKCYIILCKQKTNTKSVFLQMVYNYSAHSVDSLALSHVSLDSASHSTATTNPRPGNKTAACQNYKHLPVQVLAHTGMEKTMHASKTLHQKRSSSI gprc6a-1 1790499 N/A 1790500 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1790500 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell MYC TAG myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false F4 To determine if rGPRC6A is indeed a dimer an HTRF assay was conducted using an anti-HA antibody labeled with a donor fluorophore and an anti-c-myc labeled acceptor fluorophore (Fig. 4A). The results clearly show that rGPRC6A is capable of homodimerizing as co-expression of HA- and c-myc-tagged rGPRC6A receptors generates a FRET signal, whereas co-expression of c-myc-rGPRC6A with the unrelated HA-tagged 5-HT2A receptor did not generate a FRET signal (Fig. 4B). These experiments were performed on intact cells demonstrating that the rGPRC6A receptors dimerize on the cell surface.