MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint wang-2015-3 MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells. in vitro In vitro bn-page blue native page bn-page western blot western blot Immuno blot Immuno blot Wang X., Berry E., Hernandez-Anzaldo S., Sun D., Adijiang A., Li L., Zhang D., Fernandez-Patron C. cf2@ualberta.ca FEBS Lett. (0014-5793) 2015 Only protein-protein interactions imex curation wang-2015-1 MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells. in vitro In vitro enzymatic study enzymatic study weight by staining molecular weight estimation by staining weight by staining Wang X., Berry E., Hernandez-Anzaldo S., Sun D., Adijiang A., Li L., Zhang D., Fernandez-Patron C. cf2@ualberta.ca FEBS Lett. (0014-5793) 2015 Only protein-protein interactions imex curation wang-2015-2 MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells. in vitro In vitro enzymatic study enzymatic study ms participant Identification by mass spectrometry ms participant Wang X., Berry E., Hernandez-Anzaldo S., Sun D., Adijiang A., Li L., Zhang D., Fernandez-Patron C. cf2@ualberta.ca FEBS Lett. (0014-5793) 2015 Only protein-protein interactions imex curation wang-2015-4 MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells. in vitro In vitro zymography zymography weight by comassie molecular weight estimation by coomasie staining weight by comassie Wang X., Berry E., Hernandez-Anzaldo S., Sun D., Adijiang A., Li L., Zhang D., Fernandez-Patron C. cf2@ualberta.ca FEBS Lett. (0014-5793) 2015 Only protein-protein interactions imex curation wang-2015-5 MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells. in vitro In vitro enzymatic study enzymatic study western blot western blot Immuno blot Immuno blot Wang X., Berry E., Hernandez-Anzaldo S., Sun D., Adijiang A., Li L., Zhang D., Fernandez-Patron C. cf2@ualberta.ca FEBS Lett. (0014-5793) 2015 Only protein-protein interactions imex curation pcsk9_human Proprotein convertase subtilisin/kexin type 9 Subtilisin/kexin-like protease PC9 Neural apoptosis-regulated convertase 1 Proprotein convertase 9 PCSK9 NARC1 PSEC0052 protein protein human Homo sapiens Human MGTVSSRRSWWPLPLLLLLLLLLGPAGARAQEDEDGDYEELVLALRSEEDGLAEAPEHGTTATFHRCAKDPWRLPGTYVVVLKEETHLSQSERTARRLQAQAARRGYLTKILHVFHGLLPGFLVKMSGDLLELALKLPHVDYIEEDSSVFAQSIPWNLERITPPRYRADEYQPPDGGSLVEVYLLDTSIQSDHREIEGRVMVTDFENVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGASMRSLRVLNCQGKGTVSGTLIGLEFIRKSQLVQPVGPLVVLLPLAGGYSRVLNAACQRLARAGVVLVTAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGEDIIGASSDCSTCFVSQSGTSQAAAHVAGIAAMMLSAEPELTLAELRQRLIHFSAKDVINEAWFPEDQRVLTPNLVAALPPSTHGAGWQLFCRTVWSAHSGPTRMATAVARCAPDEELLSCSSFSRSGKRRGERMEAQGGKLVCRAHNAFGGEGVYAIARCCLLPQANCSVHTAPPAEASMGTRVHCHQQGHVLTGCSSHWEVEDLGTHKPPVLRPRGQPNQCVGHREASIHASCCHAPGLECKVKEHGIPAPQEQVTVACEEGWTLTGCSALPGTSHVLGAYAVDNTCVVRSRDVSTTGSTSEGAVTAVAICCRSRHLAQASQELQ mint homomint May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant mmp2_human 72 kDa type IV collagenase MMP2 CLG4A 72 kDa gelatinase Matrix metalloproteinase-2 Gelatinase A TBE-1 protein protein human Homo sapiens Human MEALMARGALTGPLRALCLLGCLLSHAAAAPSPIIKFPGDVAPKTDKELAVQYLNTFYGCPKESCNLFVLKDTLKKMQKFFGLPQTGDLDQNTIETMRKPRCGNPDVANYNFFPRKPKWDKNQITYRIIGYTPDLDPETVDDAFARAFQVWSDVTPLRFSRIHDGEADIMINFGRWEHGDGYPFDGKDGLLAHAFAPGTGVGGDSHFDDDELWTLGEGQVVRVKYGNADGEYCKFPFLFNGKEYNSCTDTGRSDGFLWCSTTYNFEKDGKYGFCPHEALFTMGGNAEGQPCKFPFRFQGTSYDSCTTEGRTDGYRWCGTTEDYDRDKKYGFCPETAMSTVGGNSEGAPCVFPFTFLGNKYESCTSAGRSDGKMWCATTANYDDDRKWGFCPDQGYSLFLVAAHEFGHAMGLEHSQDPGALMAPIYTYTKNFRLSQDDIKGIQELYGASPDIDLGTGPTPTLGPVTPEICKQDIVFDGIAQIRGEIFFFKDRFIWRTVTPRDKPMGPLLVATFWPELPEKIDAVYEAPQEEKAVFFAGNEYWIYSASTLERGYPKPLTSLGLPPDVQRVDAAFNWSKNKKTYIFAGDKFWRYNEVKKKMDPGFPKLIADAWNAIPDNLDAVVDLQGGGHSYFFKGAYYLKLENQSLKSVKFGSIKSDWLGC ldlr_human Low-density lipoprotein receptor LDLR protein protein human Homo sapiens Human MGPWGWKLRWTVALLLAAAGTAVGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSGDFSCGGRVNRCIPQFWRCDGQVDCDNGSDEQGCPPKTCSQDEFRCHDGKCISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDPDCEDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECIHSSWRCDGGPDCKDKSDEENCAVATCRPDEFQCSDGNCIHGSRQCDREYDCKDMSDEVGCVNVTLCEGPNKFKCHSGECITLDKVCNMARDCRDWSDEPIKECGTNECLDNNGGCSHVCNDLKIGYECLCPDGFQLVAQRRCEDIDECQDPDTCSQLCVNLEGGYKCQCEEGFQLDPHTKACKAVGSIAYLFFTNRHEVRKMTLDRSEYTSLIPNLRNVVALDTEVASNRIYWSDLSQRMICSTQLDRAHGVSSYDTVISRDIQAPDGLAVDWIHSNIYWTDSVLGTVSVADTKGVKRKTLFRENGSKPRAIVVDPVHGFMYWTDWGTPAKIKKGGLNGVDIYSLVTENIQWPNGITLDLLSGRLYWVDSKLHSISSIDVNGGNRKTILEDEKRLAHPFSLAVFEDKVFWTDIINEAIFSANRLTGSDVNLLAENLLSPEDMVLFHNLTQPRGVNWCERTTLSNGGCQYLCLPAPQINPHSPKFTCACPDGMLLARDMRSCLTEAEAAVATQETSTVRLKVSSTAVRTQHTTTRPVPDTSRLPGATPGLTTVEIVTMSHQALGDVAGRGNEKKPSSVRALSIVLPIVLLVFLCLGVFLLWKNWRLKNINSINFDNPVYQKTTEDEVHICHNQDGYSYPSRQMVSLEDDVA pcsk9_mouse Proprotein convertase subtilisin/kexin type 9 Pcsk9 Narc1 Neural apoptosis-regulated convertase 1 Proprotein convertase 9 Subtilisin/kexin-like protease PC9 protein protein mouse Mus musculus Mouse MGTHCSAWLRWPLLPLLPPLLLLLLLLCPTGAGAQDEDGDYEELMLALPSQEDGLADEAAHVATATFRRCSKEAWRLPGTYIVVLMEETQRLQIEQTAHRLQTRAARRGYVIKVLHIFYDLFPGFLVKMSSDLLGLALKLPHVEYIEEDSFVFAQSIPWNLERIIPAWHQTEEDRSPDGSSQVEVYLLDTSIQGAHREIEGRVTITDFNSVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGTSLHSLRVLNCQGKGTVSGTLIGLEFIRKSQLIQPSGPLVVLLPLAGGYSRILNAACRHLARTGVVLVAAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGKDIIGASSDCSTCFMSQSGTSQAAAHVAGIVARMLSREPTLTLAELRQRLIHFSTKDVINMAWFPEDQQVLTPNLVATLPPSTHETGGQLLCRTVWSAHSGPTRTATATARCAPEEELLSCSSFSRSGRRRGDWIEAIGGQQVCKALNAFGGEGVYAVARCCLVPRANCSIHNTPAARAGLETHVHCHQKDHVLTGCSFHWEVEDLSVRRQPALRSRRQPGQCVGHQAASVYASCCHAPGLECKIKEHGISGPSEQVTVACEAGWTLTGCNVLPGASLTLGAYSVDNLCVARVHDTARADRTSGEATVAAAICCRSRPSAKASWVQ ldlr-mmp2-1 1779426 n/a 1779431 western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component purified purified n/a 1779432 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified n/a 1779433 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region inferred by curator inferred by curator modelled by curator modeled by curator certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false f3 mmp2-pcsk9-4 1779426 N/A 1779431 unspecified role unspecified role neutral component neutral component purified purified N/A 1779432 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction false false false f3 mmp2-pcsk9 1779427 n/a 1779431 weight by comassie molecular weight estimation by coomasie staining weight by comassie enzyme target enzyme target substrate substrate neutral component neutral component purified purified n/a 1779432 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified cleavage cleavage reaction cleavage false false false f2a f2c f4a f5b mmp2-pcsk9-1 1779428 n/a 1779434 ms participant Identification by mass spectrometry ms participant enzyme target enzyme target substrate substrate neutral component neutral component purified purified gly397 modres protein modification ModRes modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false gly617 modres protein modification ModRes modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false n/a 1779432 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified cleavage cleavage reaction cleavage false false false sf2 f2b st2 two putative cleavage sites both occurring at Gly;Ile bonds proximal to the C-terminus of PCSK9 (Supplementary Fig. 2) mmp2-pcsk9-2 1779429 n/a 1779431 weight by comassie molecular weight estimation by coomasie staining weight by comassie enzyme target enzyme target substrate substrate neutral component neutral component purified purified n/a 1779432 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified cleavage cleavage reaction cleavage false false false f5a f3 mmp2-pcsk9-3 1779430 n/a 1779431 anti tag western anti tag western blot anti tag western enzyme target enzyme target substrate substrate neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1779432 western blot western blot Immuno blot Immuno blot enzyme enzyme neutral component neutral component purified purified cleavage cleavage reaction cleavage false false false f5c