IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ yin-2014-3 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Yin G., Lopes da Fonseca T., Eisbach SE., Anduaga AM., Breda C., Orcellet ML., Szegő ÉM., Guerreiro P., Lázaro DF., Braus GH., Fernandez CO., Griesinger C., Becker S., Goody RS., Itzen A., Giorgini F., Outeiro TF., Zweckstetter M. Parkinsons - Interactions investigated in the context of Parkinsons disease Results from Figure 6 and Supp 4 not included as the origin of GFP-Rab8A is not stated. Sequence mapping of Rab8a is given in relation to Uniprot P61006, rather than residues given here- notabley for "A173-G181" and " I193-T194". markus.zweckstetter@dzne.de,tiago.outeiro@med.uni-goettingen.de Neurobiology of disease 2014 imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Only protein-protein interactions yin-2014-1 mouse-synaptosome Mouse synaptosome synaptosome Synaptosome anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yin G., Lopes da Fonseca T., Eisbach SE., Anduaga AM., Breda C., Orcellet ML., Szegő ÉM., Guerreiro P., Lázaro DF., Braus GH., Fernandez CO., Griesinger C., Becker S., Goody RS., Itzen A., Giorgini F., Outeiro TF., Zweckstetter M. Parkinsons - Interactions investigated in the context of Parkinsons disease Results from Figure 6 and Supp 4 not included as the origin of GFP-Rab8A is not stated. mouse cortical synaptosomes markus.zweckstetter@dzne.de,tiago.outeiro@med.uni-goettingen.de Neurobiology of disease 2014 imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Only protein-protein interactions yin-2014-4 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Yin G., Lopes da Fonseca T., Eisbach SE., Anduaga AM., Breda C., Orcellet ML., Szegő ÉM., Guerreiro P., Lázaro DF., Braus GH., Fernandez CO., Griesinger C., Becker S., Goody RS., Itzen A., Giorgini F., Outeiro TF., Zweckstetter M. Parkinsons - Interactions investigated in the context of Parkinsons disease Results from Figure 6 and Supp 4 not included as the origin of GFP-Rab8A is not stated. ThT is added as a dye to visualize and quantify the presence or fibrilization of misfolded protein aggregates. markus.zweckstetter@dzne.de,tiago.outeiro@med.uni-goettingen.de Neurobiology of disease 2014 imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Only protein-protein interactions yin-2014-5 in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Yin G., Lopes da Fonseca T., Eisbach SE., Anduaga AM., Breda C., Orcellet ML., Szegő ÉM., Guerreiro P., Lázaro DF., Braus GH., Fernandez CO., Griesinger C., Becker S., Goody RS., Itzen A., Giorgini F., Outeiro TF., Zweckstetter M. Parkinsons - Interactions investigated in the context of Parkinsons disease Results from Figure 6 and Supp 4 not included as the origin of GFP-Rab8A is not stated. markus.zweckstetter@dzne.de,tiago.outeiro@med.uni-goettingen.de Neurobiology of disease 2014 imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Only protein-protein interactions yin-2014-2 rat-hippocampus Rattus norvegicus hippocampal neurons hippocampus Hippocampus [Brain hippocampus] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yin G., Lopes da Fonseca T., Eisbach SE., Anduaga AM., Breda C., Orcellet ML., Szegő ÉM., Guerreiro P., Lázaro DF., Braus GH., Fernandez CO., Griesinger C., Becker S., Goody RS., Itzen A., Giorgini F., Outeiro TF., Zweckstetter M. Parkinsons - Interactions investigated in the context of Parkinsons disease Results from Figure 6 and Supp 4 not included as the origin of GFP-Rab8A is not stated. mouse cortical synaptosomes markus.zweckstetter@dzne.de,tiago.outeiro@med.uni-goettingen.de Neurobiology of disease 2014 imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Only protein-protein interactions yin-2014-6 human-h4 Homo sapiens neurogioma cell line h4 Human neuroglioma fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Yin G., Lopes da Fonseca T., Eisbach SE., Anduaga AM., Breda C., Orcellet ML., Szegő ÉM., Guerreiro P., Lázaro DF., Braus GH., Fernandez CO., Griesinger C., Becker S., Goody RS., Itzen A., Giorgini F., Outeiro TF., Zweckstetter M. Parkinsons - Interactions investigated in the context of Parkinsons disease Results from Figure 6 and Supp 4 not included as the origin of GFP-Rab8A is not stated. markus.zweckstetter@dzne.de,tiago.outeiro@med.uni-goettingen.de Neurobiology of disease 2014 imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Only protein-protein interactions gdp guanosine 5-diphosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) rab8a_human Ras-related protein Rab-8A Oncogene c-mel RAB8A MEL RAB8 protein protein human Homo sapiens Human MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSNQGVKITPDQQKRSSFFRCVLL syua_human Alpha-synuclein Non-A beta component of AD amyloid Non-A4 component of amyloid precursor SNCA NACP PARK1 protein protein human Homo sapiens Human MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA guanosine 5'-[beta,gamma-imido]triphosphate guanosine 5'-[beta,gamma-imido]triphosphate 5'-Guanylic acid, monoanhydride with imidodiphosphoric acid 5'-guanylylimidodiphosphate GMP-PNP GMP-Pnp Gpp(NH)-p Gpp(NH)p Guanylyl imidodiphosphate 5'-O-(hydroxy{[hydroxy(phosphonoamino)phosphoryl]oxy}phosphoryl)guanosine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) rab8a_mouse Ras-related protein Rab-8A Rab8a Mel Rab8 Oncogene c-mel protein protein mouse Mus musculus Mouse MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSSHGVKITVEQQKRTSFFRCSLL syua_mouse Alpha-synuclein Snca Syn Non-A beta component of AD amyloid Non-A4 component of amyloid precursor protein protein mouse Mus musculus Mouse MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVTTVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGFVKKDQMGKGEEGYPQEGILEDMPVDPGSEAYEMPSEEGYQDYEPEA rab8a_rat Ras-related protein Rab-8A Rab8a Rab8 protein protein rat Rattus norvegicus (Rat) Rat MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSSHGVKITVEQQKRTSFFRCSLL syua_rat Alpha-synuclein Snca protein protein rat Rattus norvegicus (Rat) Rat MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVTTVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGFVKKDQMGKGEEGYPQEGILEDMPVDPSSEAYEMPSEEGYQDYEPEA rab8a-snca-1 1857358 N/A 1857364 cofactor cofactor coenzyme neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 1857365 unspecified role unspecified role neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1857366 unspecified role unspecified role neutral component neutral component undefined mutation mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ser129 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1857377 1857374 direct interaction direct interaction false false false 1857358 2A, 4, Supp 2B gdp-rab8a-1 1857358 N/A 1857367 cofactor cofactor coenzyme neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 1857365 unspecified role unspecified role neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1857366 unspecified role unspecified role neutral component neutral component region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ser129 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1857388 1857385 1857392 1857388 direct interaction direct interaction false false false 1857358 2B, Supp 2B gdp-rab8a-3 1857358 N/A 1857364 cofactor cofactor coenzyme neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 1857365 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 1857366 unspecified role unspecified role neutral component neutral component ser129 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) 1857397 1857395 1857401 1857397 direct interaction direct interaction false false false 1857358 Supp 2C gdp-rab8a-4 1857358 N/A 1857364 cofactor cofactor coenzyme neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 1857365 unspecified role unspecified role neutral component neutral component region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false alpha2helix_switch_regions binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c_terminal_region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1857366 unspecified role unspecified role neutral component neutral component undefined mutation mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false ser129 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1857409 1857404 direct interaction direct interaction false false false 1857358 3 snca-rab8a-1 1857359 N/A 1857368 unspecified role unspecified role prey prey N/A 1857369 unspecified role unspecified role bait bait physical association physical association false false false 1A snca-rab8a-2 1857359 N/A 1857368 unspecified role unspecified role bait bait N/A 1857369 unspecified role unspecified role prey prey physical association physical association false false false 1B snca-snca-13 1857360 N/A 1857364 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1857365 stimulator stimulator neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1857366 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1857366 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1857425 1857422 1857429 1857427 physical association physical association false false false 5A gdp-rab8a-5 1857360 N/A 1857366 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1857366 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1857434 1857432 physical association physical association false false false 5Ai gdp-rab8a-2 1857361 N/A 1857364 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1857365 stimulator stimulator neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 1857366 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1857366 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1857439 1857437 1857444 1857442 physical association physical association false false false 5B snca-snca-14 1857361 N/A 1857366 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1857366 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1857449 1857447 physical association physical association false false false 5Bi snca-rab8a-3 1857362 N/A 1857370 unspecified role unspecified role prey prey N/A 1857371 unspecified role unspecified role bait bait physical association physical association false false false 1A 4235rw3-1 1857363 N/A 1857366 unspecified role unspecified role neutral component neutral component N/A 1857365 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Supp 4A