IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ esakova-2013-1 in vitro In vitro crosslink cross-linking study crosslink primer extension assay primer extension assay primer extension assay Esakova O., Perederina A., Berezin I., Krasilnikov AS. Protein tags were used only to verify the identity of each protein. Infact MRP complexes were isolated from yeast strains carrying a single protein tagged per time. The elettrophoretic mobility shift compared to wt helps in identify the protein (see fig4C) The complex is first immunoprecipitaded with rmp1 as a bait, then it is UV crosslinked in the presence of a synthetic oligo and analysed. ask11@psu.edu Nucleic acids research 2013 imex curation Only protein-protein interactions nme1_yeast_rna NME1 RNA component of RNase MRP rna ribonucleic acid RNA rna yeast Saccharomyces cerevisiae Baker's yeast AAUCCAUGACCAAAGAAUCGUCACAAAUCGAAGCUUACAAAAUGGAGUAAAAUUUUUUUUACUCAGUAAUAUGCUUUGGGUUGAAAGUCUCCCACCAAUUCGUAUGCGGAAAACGUAAUGAGAUUUAAAAAUUUUAAAUUGUUUAAAUCAACUCAUUAAGGAGGAUGCCCUUGGGUAUUCUGCUUCUUGACCUGGUACCUCUAUUGCAGGGUACUGGUGUUUUCUUCGGUACUGGAUUCCGUUUGUAUGGAAUCUAAACCAUAGUUAUGACGAUUGCUCUUUCCCGUGCUGGAUCGAGUAACCCAAUGGAGCUUACUAUUCUUGGUCCAUGGAUUCACCC Cross reference is to gene rather than to RNA mrp substrate synthetic MRP substrate nucleic acid nucleic acid synthetic construct synthetic construct GGCAACAAAUUUUUUAACACAAA rmp1_yeast Ribonuclease MRP protein subunit RMP1 RNase MRP 23.6 kDa subunit RNA-processing protein RMP1 RMP1 YLR145W protein protein yeast Saccharomyces cerevisiae Baker's yeast MDEMDNVIRSLEQEYRLILLLNHRNKNQHRAASWYGSFNEMKRNCGQIITLFSSRRLQAKRLKDVEWVKLHRLLQRALFRQLKRWYWQFNGVIALGQFVTLGCTLVTLLANVRALYMRLWEINETEFIRCGCLIKNLPRTKAKSVVNDVEELGEIIDEDIGNNVQENELVITSIPKPLTENCKKKKKRKKKNKSAIDGIFG pop5_yeast Ribonuclease P/MRP protein subunit POP5 POP5 RNA-processing protein POP5 RNase P/MRP 19.6 kDa subunit FUN53 YAL033W protein protein yeast Saccharomyces cerevisiae Baker's yeast MVRLKSRYILFEIIFPPTDTNVEESVSKADILLSHHRASPADVSIKSILQEIRRSLSLNLGDYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALMLMSKIGDVDGLIVNPVKVSGTIKKIEQFAMRRNSKILNIIKCSQSSHLSDNDFIINDFKKIGRENENENEDD 918193631BD790DD pop4_yeast RNases MRP/P 32.9 kDa subunit POP4 YBR257W YBR1725 RNA-processing protein POP4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDRTQTFIKDCLFTKCLEDPEKPFNENRFQDTLLLLPTDGGLTSRLQRQQRKSKLNLDNLQKVSQLESADKQLEKRDYQRINKNSKIALREYINNCKKNTKKCLKLAYENKITDKEDLLHYIEEKHPTIYESLPQYVDFVPMYKELWINYIKELLNITKNLKTFNGSLALLKLSMADYNGALLRVTKSKNKTLIGLQGIVIWDSQKFFIMIVKGNIIDEIKCIPKKGTVFQFEIPISDDDDSALRYSILGDRFKYRSVDRAGRKFKSRRCDDMLYYIQN rpp1_yeast Ribonuclease P/MRP protein subunit RPP1 RPP1 RNA-processing protein RPP1 RNaseP/MRP 32.2 kDa subunit YHR062C protein protein yeast Saccharomyces cerevisiae Baker's yeast MLVDLNVPWPQNSYADKVTSQAVNNLIKTLSTLHMLGYTHIAINFTVNHSEKFPNDVKLLNPIDIKRRFGELMDRTGLKLYSRITLIIDDPSKGQSLSKISQAFDIVAALPISEKGLTLSTTNLDIDLLTFQYGSRLPTFLKHKSICSCVNRGVKLEIVYGYALRDVQARRQFVSNVRSVIRSSRSRGIVIGSGAMSPLECRNILGVTSLIKNLGLPSDRCSKAMGDLASLVLLNGRLRNKSHKQTIVTGGGSGNGDDVVNDVQGIDDVQTIKVVKRSMDAEQLGHASKRHKP pop1_yeast Ribonucleases P/MRP protein subunit POP1 POP1 YNL221C N1285 RNases P/MRP 100.4 kDa subunit RNA-processing protein POP1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGSLSRGNGGKKVLNKNQLLKRNRIRNARSIRAEAVAASSTKTGTPSDLSESGSKLNVDQFISSRQFEVKQLQLAMHNSKAASSTRIFQALPRKLRRRTASHNVRRIPKRMRNRALREMRKSDQQDVLKGSSASSRKAHGLNAKQLYKARMSIKLLRLASKSTSMKLSMPPEVTSSNCHVRQKIKTLKRMIKESSTANPNIKLLNNRMGSYDCTGVNELAPIPKGRVKYTKRQKHFAWLPTHIWNAKRSHMMKRWGYQMVWAPTQKCFKLTHRLGGDTCSSDGALCMDSSYIGTIIVKDKSNDSEGDFLKSIIGKLTAERANLRKYREGQVLFQGLIYSFNEENGEDSTKPLGPCDVFWVQKDTAIIRLHPSIYTQVFNILLQHKEKLTVQDCRYSLASVTLKGAKALESLASCLRSTEYSKSFEQFKMVSMITDHNALPQRCTFAFEAIDPRHLAAPKKLNDSQRKTVNSDDILSLHENYPQDEINAVFNELCDPESRTQSYNNQNTLKEISARRYKLLTATPNSINKTTVPFKESDDPSIPLVIIRRLKTRDWIVVLPWFWLLPLWHLLNRIPRMYHIGLRQFQQIQYENKQLYFPDDYPFTQLGYIENSFYKKEASKTKWDRKPMGKRINFEKIKDIHNTKLPAYSGEIGDFFSSDWRFLQILRNGIDYLQRNDKTLELMDSKKTGQFNAQGVRDINCVNDVLEFCKDYEAKTKAMSLSIEENIPVALCKNRKCQFRTPDSISVNSSSFSLTFFPRCIIAVSCTLLERGHPKDNARIYQVPEKDLEHWLQLAKGVYRPNGRKDHDLKIPLPEVHDLIGFITSGTYHLNCGNGMGIGFIDHHAAIRQPTRYVLIRNVGTNTYRLGEWSKISV mrp_nme1-1 1883397 N/A 1883398 unspecified role unspecified role neutral component neutral component region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 1883399 unspecified role unspecified role neutral component neutral component label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1883400 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component tag protein A His tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 1883401 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component tag fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 1883402 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component tag fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 1883403 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component tag fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 1883404 sequence tag sequence tag identification sequence tag unspecified role unspecified role neutral component neutral component tag fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 1883422 1883411 physical association physical association false false false Fig3, Fig4 Proteinase K was used to discriminate between RNA-RNA and RNA-protein complexes, when comigrating.