IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ venuprasad-2008-1 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Venuprasad K., Huang H., Harada Y., Elly C., Subramaniam M., Spelsberg T., Su J., Liu YC. IBD - Inflammatory bowel disease CD4+CD25- cells yuncail@liai.org Nature immunology 2008 Only protein-protein interactions imex curation imex curation venuprasad-2008-2 mouse-t-cell Mouse T cell t-cell Peripheral blood T-lymphocytes anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Venuprasad K., Huang H., Harada Y., Elly C., Subramaniam M., Spelsberg T., Su J., Liu YC. IBD - Inflammatory bowel disease CD4+CD25- cells stimulated with TGF-beta. yuncail@liai.org Nature immunology 2008 Only protein-protein interactions imex curation imex curation venuprasad-2008-3 in vitro In vitro emsa supershift electrophoretic mobility supershift assay emsa supershift autoradiography autoradiography Venuprasad K., Huang H., Harada Y., Elly C., Subramaniam M., Spelsberg T., Su J., Liu YC. IBD - Inflammatory bowel disease CD4+CD25- T cells stimulated with anti-CD3/28 + TGF-beta. In figure 4E: HA-Ubiquitin is of Human origin- see pmid:8087846 (obtained via Ref39-Ref13 from there.) ITCH is of Murine origin, although no mention of an Xpress tag can be found in references (39,40) provided. TIEG1 is of Murine origin, as used in this publication. yuncail@liai.org Nature immunology 2008 Only protein-protein interactions imex curation imex curation venuprasad-2008-4 mouse-t-cell Mouse T cell t-cell Peripheral blood T-lymphocytes ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Venuprasad K., Huang H., Harada Y., Elly C., Subramaniam M., Spelsberg T., Su J., Liu YC. IBD - Inflammatory bowel disease CD4+CD25- T cells stimulated with anti-CD3/28 + TGF-beta. yuncail@liai.org Nature immunology 2008 Only protein-protein interactions imex curation imex curation itch_mouse E3 ubiquitin-protein ligase Itchy Itch HECT-type E3 ubiquitin transferase Itchy homolog protein protein mouse Mus musculus Mouse MSDSGPQLDSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPTSKLCFRVWSHQTLKSDVLLGTAGLDIYETLKSNNMKLEEVVMTLQLVGDKEPTETMGDLSVCLDGLQVEAEVVTNGETSCSESTTQNDDGCRTRDDTRVSTNGSEDPEVAASGENKRANGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTNSDSDGSSTGSLPPTNTNVNTSTSEGATSGLIIPLTISGGSGPRPLNTVSQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRATTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE klf10_mouse Krueppel-like factor 10 Gdnfif Tieg Tieg1 Klf10 GDNF-inducible factor Transcription factor GIF Transforming growth factor-beta-inducible early growth response protein 1 protein protein mouse Mus musculus Mouse MLNFGASLQQASEGKMELISEKPREGMHPWDKAEQSDFEAVEALMSMSCDWKSHFKKYLENRPVTPVSDTSEDDSLLPGTPDLQTVPAFCLTPPYSPSDFEPSQGSNLTASAPSTGHFKSFSDAAKPPGATPFKEEEKNPLAAPPLPKAQATSVIRHTADAQLCNHQSCPVKAASILNYQDNSFRRRTHGNVEATRKNIPCAAVSPNRSKPEPSTVSDGDEKAGAALYDFAVPSSETVICRSQPAPSSPVQKSVLVSSPTVSTGGVPPLPVICQMVPLPANNSLVSTVVPSTPPSQPPAVCSPVLFMGTQVPEGTVVFVVPQPVVQSPRPPVVSPSGTRLSPIAPAPGFSPSAARVTPQIDSSRVRSHICSHPGCGKTYFKSSHLKAHVRTHTGEKPFSCSWKGCERRFARSDELSRHRRTHTGEKKFACPMCDRRFMRSDHLTKHARRHLSAKKLPNWQMEVSKLNDIALPPTPASAQ sp1_binding_region_mouse-foxp3_promoter ds dna double stranded deoxyribonucleic acid ds DNA ds dna mouse Mus musculus Mouse AAAACTACAAGAACCCCCCCCCCACCCTGCAATTATCAGCAC ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG foxp3_mouse_gene Forkhead box protein P3 Foxp3 gene gene mouse Mus musculus Mouse klf10-itch-1 1857102 N/A 1857106 unspecified role unspecified role prey prey ww_domains sufficient to bind sufficient binding region sufficient to bind range range range range false mouse-t-cell Mouse T cell t-cell Peripheral blood T-lymphocytes N/A 1857107 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 3 B/C, Supp 5A TGF-beta itch-klf10-1 1857102 N/A 1857106 unspecified role unspecified role bait bait ww_domains sufficient to bind sufficient binding region sufficient to bind range range range range false n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 1857107 unspecified role unspecified role prey prey mouse-t-cell Mouse T cell t-cell Peripheral blood T-lymphocytes physical association physical association false false false 3D Reference 39 states: (Itch) the four WW domains (WW from Pro268 to Pro471). However in the sequence given in UniProt Q8C863 the nearest Proline residues are at residues 269 and 470, so these have been entered. itch-klf10-2 1857103 N/A 1857106 unspecified role unspecified role bait bait N/A 1857107 unspecified role unspecified role prey prey physical association physical association false false false 3E, Supp 5A klf10-itch-2 1857103 N/A 1857106 unspecified role unspecified role prey prey N/A 1857107 unspecified role unspecified role bait bait physical association physical association false false false 3E, supp 5A sp1_region_mouse_foxp3_promoter-klf10-1 1857104 N/A 1857107 unspecified role unspecified role prey prey mouse-t-cell Mouse T cell t-cell Peripheral blood T-lymphocytes N/A 1857108 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 4 B/C DNA-binding ability was almost completely abolished in TGF-beta-stimulated Itch-/- T cells. sp1_binding_region_mouse_foxp3_promoter-klf10-2 1857104 N/A 1857109 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1857107 unspecified role unspecified role prey prey human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 1857108 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 1857106 unspecified role unspecified role prey prey human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. association association false false false 4 E foxp3-klf10-1 1857105 N/A 1857107 unspecified role unspecified role bait bait N/A 1857110 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 4 D/G Q8C863 is required for the binding of O89091 to ENSMUSG00000039521 Q8C863 increases the expression at protein level of ENSMUSG00000039521 O89091 increases expression at protein level of ENSMUSG00000039521 O89091 is required for the expression at protein level of ENSMUSG00000039521