IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hann-1991-1 yeast Saccharomyces cerevisiae Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip northern blot northern blot northen Hann BC., Walter P. Cell 1991 imex curation Only protein-protein interactions imex curation hann-1991-2 yeast Saccharomyces cerevisiae Baker's yeast cosedimentation cosedimentation northern blot northern blot northen Hann BC., Walter P. scr1 yeast RNA and srp54 were fist co-immunoprecipitated prior to cosedimentation Cell 1991 imex curation Only protein-protein interactions imex curation scr1 yeast Signal Recognition Particle RNA yeast srprna signal recognition particle rna srpRNA srprna yeast Saccharomyces cerevisiae Baker's yeast AGGCUGUAAUGGCUUUCUGGUGGGAUGGGAUACGUUGAGAAUUCUGGCCGAGGAACAAAUCCUUCCUCGCGGCUAGACACGGAUUGCACGCCCUUUGGGCAAGGGAUAGUUCUCUAUUCCGCACCGUGCCCUGUUGUGGCAACCGUCUUUCCUCCGUCGUAAAUUUGUCCUGGGCAGAGCUGUCUGCCCGGAGGCGGGAGAGUCCGUUCUGAAGUGUCCCGGCUAUAAUAAAUCGAUCUUUGCGGGCAGCCCGUUGGCAGGAGGCGUGAGGAAUCCGUCUCUCUGUCUGGUGCGGCAAGGUAGUUCUGGGUCCUUAGGGGCUCCACCUUCACCGCUGUUAGGGGAGUUUUAUCCAGCGUCAGCAAAGGUGACCCGUGAUGGAGGCGGCCGGGAUAGCACAUAUCAGUCGGAUAAUUGUGCAAGUUGAUCGCUUCGGCGGUUUAAUUUGGCGGUGCCAUCAGGAUUUACUCGCACAUUGUGGCCGUUCCCUCGGGGAUGGAGUGUGUCCUGAACCAUAUUUUU srp54_yeast Signal recognition particle subunit SRP54 SRH1 P9513.14 YPR088C SRP54 Signal recognition particle 54 kDa protein homolog protein protein yeast Saccharomyces cerevisiae Baker's yeast MVLADLGKRINSAVNNAISNTQDDFTTSVDVMLKGIVTALLESDVNIALVSKLRNNIRSQLLSENRSEKSTTNAQTKKLIQKTVFDELCKLVTCEGSEEKAFVPKKRKTNIIMFVGLQGSGKTTSCTKLAVYYSKRGFKVGLVCADTFRAGAFDQLKQNAIRARIPFYGSYTETDPAKVAEEGINKFKKEKFDIIIVDTSGRHHQEEELFQEMIEISNVIKPNQTIMVLDASIGQAAEQQSKAFKESSDFGAIILTKMDGHARGGGAISAVAATNTPIIFIGTGEHIHDLEKFSPKSFISKLLGIGDIESLFEQLQTVSNKEDAKATMENIQKGKFTLLDFKKQMQTIMKMGPLSNIAQMIPGMSNMMNQVGEEETSQKMKKMVYVLDSMTKEELESDGRMFIEEPTRMVRVAKGSGTSVFEVEMILMQQQMMARMAQTATQQQPGAPGANARMPGMPNMPGMPNMPGMPNMPGMPKVTPQMMQQAQQKLKQNPGLMQNMMNMFGGGMGGGMGGGMPDMNEMMKMMQDPQMQQMAKQFGMG 05B1B2C262932F17 scr1_srp54-1 1861018 N/A 1861020 unspecified role unspecified role prey prey N/A 1861021 unspecified role unspecified role bait bait physical association physical association false false false Fig2 an excess of spr54 (which saturates the antibody), inhibits the IP. scr1 yeast-srp54-1 1861019 N/A 1861020 unspecified role unspecified role neutral component neutral component N/A 1861021 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig3 The binding was confirmed by co-immunoprecipitation of the sedimentation fraction fig3 D