IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ prochasson-2005-1 yeast Saccharomyces cerevisiae Baker's yeast tap tandem affinity purification TAP TAP tap mudpit multidimensional protein identification technology mudpit Prochasson P., Florens L., Swanson SK., Washburn MP., Workman JL., Prochasson P., Florens L., Swanson SK., Washburn MP., Workman JL. jlw@stowers-institute.org Genes & development 2005 Only protein-protein interactions imex curation imex curation prochasson-2005-2 yeast Saccharomyces cerevisiae Baker's yeast density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Prochasson P., Florens L., Swanson SK., Washburn MP., Workman JL., Prochasson P., Florens L., Swanson SK., Washburn MP., Workman JL. jlw@stowers-institute.org Genes & development 2005 Only protein-protein interactions imex curation imex curation hpc2_yeast Histone promoter control protein 2 YBR1503 YBR215W HPC2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDQKAIVLDNSKSGSKQTKSSGKMQTQTDTNAEVLNTDNSIKKETGSDSEDLFNKFSNKKTNRKIPNIAEELAKNRNYVKGASPSPIIISGSSSTSPSGPSSSSTNPMGIPTNRFNKNTVELYQHSPSPVMTTNKTDTEEKRQNNRNMDNKNTPERGSSSFAAKQLKISSLLTISSNEDSKTLHINDTNGNKNSNAASNNIPSAYAELHTEGNSIESLIKPPSSPRNKSLTPKVILPTQNMDGTIAKDPHLGDNTPGILIAKTSSPVNLDVESTAQSLGKFNKSTNSLKAALTKAPAEKVSLKRSISSVTNSDSNISSSKKPTSEKAKKSSSASAILPKPTTTKTSKKAASNSSDSTRKKNASNKTTSAIKKESNAGSKLNTVKKENSSLSSIKATEKEKDKGGNSTEAKNSTSNVRKEPTAKSPKRLVAAPTVSPPKILQTAETKAKEPSILIDVPLYQADTNDYLDENGQVIFNLSTLIKEKYHPKSKELAQLKDSKRNLLMQLSDHSNGSLEKEKDEEGDVIELDDDEDMEEDEGEIDTETNTVTTTISPKKKSHPMKGKNLIGKYDVEDPFIDDSELLWEEQRAATKDGFFVYFGPLIEKGHYASLERANGTMKRGGVKNK hir3_yeast Histone transcription regulator 3 YJR140C J2161 HIR3 HPC1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSMFNALNSNIEGEQYEAEEHSRELQIEQSFNILQDALIDLKNKDFEKSDSKFQELFQIDVVKPDRWGMYRNSSPTLDNLRYLCYRNRGMYYHLYLENNYERLNSQELVNCILKAVENLVESIQHSDADFAVTDLLARIFKSFNSVKLERLISEYEFTKQENLSLLLGRHRKFLLNDLTLMMNNYVELTNKLLVPNLSDNTIFERYHLEKYKDIKPEPLAFGPILSRISEMKKQDEEIMKKLDVFNVTLNEESWDEVAKALKNLLPSVKTSSLIGRNMDPYNEIEEPIEAVKFELSEAINNTPSLDRESERQEEEQDNESVRADDKSGNLAPSDIQTNEEARPNKRTDEHIDSTKPLQRSSKRFKEREQENSKELVMDVHKRFFGEFNTLLSYIHILPFCDFDTFASKFIIGSSDKQPEKFIPYTDLYECLKSWSSRYTDIFNQNDYLSSGSNENEELFQLNALLKSNAFDDKESFPRYLNDLDSDHIRSFISEVNAGNLHFHQVRLKLLFKLLGTYDEGNGRRLIIDYLWESQLLKIVLWFVFGIESNIFALINKNKRQCKYLALSIYELLVNHLGNIVEEITNKRIQGHKSADLKSQRNKVEKRIRSWHTLLEQIADEKDKELYVHFQWTHYCFLQYTCDIVDSRLSETLTSLENTIKDSDSSLDIAYPNYRHIPALNLNTVQSQKRKIRIIQNITVEDISEDTNSDTHSENHLETLEKVLLHILHPSTNHSNIDEEMVSFIFNSPFLLKIRLWGVLFSSYVKKSSIQDVQRIYFHVLDFMKGALTSPVYKESNPHGRHQMLLTVLTAIGYLSSQLTAILNSNRWESSDFVLEDYMFEKLLQTFFFFYTVLFYESSAVNDVSNKSFFKRASKSSGKMKDIMIDLATLILYYYDLQAKLRTPAEQGIETTELIWSLHTLFGHFHFCDASNGKFLDLAEKLLCQFINNDSFLQLKQILWCRYHYAIASDNFSPDLHDTKAVEMEKIHSLPLGTYLIKLQYQNKNPYLSSSKTTLKQIMDNIIEKIGDPSTLDNHIISRNSFLLNEYLSRPITADLLKHTFSGATSLYLTSPNDELQQGMTAGLFYVSSLQSLGLYKMRKKSMQARPSELDSIIRMLKNDIIYNTNRFESWILLGKCYSYIVEDDLIWTSDKITVPEKKDVIALTQRKAILCYLMAISIYYSKLDRTIDDKKIILEALDDLGSMLISGYYNPMNKLCFSWKSSAENTMRLSETGEVVMEKTKKITTISDFNIEQSIFLCFNRACSLSGDIKSQDDVFVLNWSSFYNLAKFFFKTDGGNNCKLVAKYITQGCQIAYESSPAKDPIIEPHYLLVNACYKWVKRGVIGVNEALTLLSKDNQFFQEQEEFWVNDEGLAWDYQEKFFFDKIIRLLRHLLSVDKKKWQHRPRYRIARILFDDLGDVNGALEEMDSLISAKSINKNLVNIWKPDFERPGKHFIYTYQYLVLYLDLLFAIKDFNTTGLVIKKLRRFGSGTVNVNELLERAINVYTQSAKIKLQLQDKSYVEQILPTLNYQEFLKISEQLNQVFDQGKYPEEISSGLKLAFQLKKGHSGIAFDSVCLGIYFEYLYFPLARQDQSLTDVNDENNPALPSSGSVTSKSTPDPTSKPSAIKKRVTKKEVFDRVRLLVDKIT B1F31815797852D4 hir2_yeast Protein HIR2 HIR2 YOR038C OR26.31 Histone transcription regulator 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MRLLKYPLDIHNEQVNALAALGPYIILAGSGGHVMAWRQQQLVDTAFDRVMIKDLKPEVSFQVDQDTTGDIFFITGDLETLYIGSEHRLWGYSGWLCRDTNNINSVEKMNSKLLFECKSPSTITDVKYDINLGILFVLLSNENKILLFRHKTFDKLSEITIDKASKPITGIIDPTGQTFTVMTSDRSILVYQINKTGTHKLINKLTQHVQMYPLHYRISMSPQADILPVINSVKGVPNNATSCTALLDRNNNYKVTKTLVTPSSNGCRVLVYSPAFYEKPNLKKGTSTRYNLIATSGSTDGTILVWNTKRMKPLFNALQVSSTAINDMSWSQDGFTLFAISNDATLYTFAFQEKDLGVALPQTEIKSLQEVNKKLPKLEEPLAEQIPKSFPENIKLEESASAAPIPNDIGRSAVGKKPTKKKTANNQTNGIKTIQSTSMEFNTPSYTVPRDLKRKPKEATPSNIAPGSKKQKKELQPIDFLDTGLLLPNTSFSRIRLATPKIRSTFKYSPINNPNLILDVKNGSGNEQRPTIVKLTSKVLDQDQVLFQDFIPKLITICTAGDTFWSFCSEDGSIYIYSDSGRKLMAPLVLGVSISFLEACGTYLLCLTSIGELYCWNIEQKKLAFPTNTIYPLLNPSLRYSDDILTRAENITLCSITKKGVPLVTLSNGDGYLFDKNMETWLLVSDGWWAYGSQYWDTTNTTGLSSSKANTDSFNGSESNINEIVSDIKNDNQSIINFLECKTNDELNRKGRIKNLQRFARTILMKEGFENMEEIVTLSHLENKILISIRLEEPEEFSKLMMVYCIRLSELGYMDRLNDVFQWLYDDLPISGTGSAFADKDFKRNLLKKILIACGDIRQVQRVTTRYAKEMNIIS C77D677BF424680D hir1_yeast Protein HIR1 HIR1 YBL008W YBL0318 Histone transcription regulator 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKVVKFPWLAHREESRKYEIYTVDVSHDGKRLATGGLDGKIRIWSIDSILRCMELESLTPEIPLPQDLQMPLCSMSRHTGSITCVKFSPDGKYLASGSDDRILLIWALDEEQSSQPAFGSEHEREHWTVRKRLVAHDNDIQDICWAPDSSILVTVGLDRSVIVWNGSTFEKLKRFDVHQSLVKGVVFDPANKYFATTSDDRTMKIFRYHKTGDISFTIEHIITEPFKESPLTTYFRRPSWSPDGQHIAVPNATNGPVSSVAIVNRGTWDTNVSLIGHDAPTEVARFNPRLFERNAGVKQKKDDDPENALVGQNDDKVHHFDKNIDSVVATAGQDKSLAVWSTSRPRPILVAFDIANKSITDMSWNPDGSLLFVASLDSSITLFKFENNELGKPIPLEKNMEQLYRYGVDKDSLDFPESINQLLLEDQTKSFKHTKISTSKLGENHPTLATNSASNQKDNNDASVSRSEHINILIPKRKKDAILNKAVTLKSGKKRVAPTLISTSSSSPFSNGIKKPTLDSKRIENNVKSSTKTINSKNTLLNVPEGVEKKISISSFPLPRLGIHSLIMGTKERSAWKISNSELENDDADNAGGKGSDGTSNSIDDIAVLSEEENDFHRMTLNAKLTQEKIWSEEPTTRCLLQSDVIPDTDVVVLEGGSLDDIAVLEIRNGVERSIQFDSEALLDNPTRILGYQGGKRTIETFIPEVIICAIGSKDCKCWCLASANGSIYILSYNGQQRIPKICLGHKVIKMVTSSKYLLVLTERGLFFAWDLLDLKLVLRNVPILPILNGQPIHGNKVRINKVIKCFRLDGSSCDLLLEVGDPKNVYKWTKDLGCWSLYK 40A2932F3306358A hir1-hir2-3 1852620 N/A 1852622 unspecified role unspecified role prey prey Stoichiometry: 2.0 N/A 1852623 unspecified role unspecified role prey prey Stoichiometry: 1.0 N/A 1852624 unspecified role unspecified role prey prey Stoichiometry: 2.0 N/A 1852625 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 association association false false false Fig. 1B, Supp. Table 1 Trace amounts, as estimated by spectral count, of other proteins, mainly ribosomal and heat shock proteins were also identified but were not assumed by the authors to be part of the complex. Stoichiometry was estimated by spectral counting. hir2-hir1-1 1852620 N/A 1852622 unspecified role unspecified role prey prey Stoichiometry: 2.0 N/A 1852623 unspecified role unspecified role prey prey Stoichiometry: 1.0 N/A 1852624 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 2.0 N/A 1852625 unspecified role unspecified role prey prey Stoichiometry: 1.0 association association false false false Fig. 1B, Supp. Table 1 Trace amounts, as estimated by spectral count, of other proteins, mainly ribosomal and heat shock proteins were also identified but were not assumed by the authors to be part of the complex. Stoichiometry was estimated by spectral counting. hir3-hir1-1 1852620 N/A 1852622 unspecified role unspecified role prey prey Stoichiometry: 2.0 N/A 1852623 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 N/A 1852624 unspecified role unspecified role prey prey Stoichiometry: 2.0 N/A 1852625 unspecified role unspecified role prey prey Stoichiometry: 1.0 association association false false false Fig. 1B, Supp. Table 1 Trace amounts, as estimated by spectral count, of other proteins, mainly ribosomal and heat shock proteins were also identified but were not assumed by the authors to be part of the complex. Stoichiometry was estimated by spectral counting. hpc2-hir1-1 1852620 N/A 1852622 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 2.0 N/A 1852623 unspecified role unspecified role prey prey Stoichiometry: 1.0 N/A 1852624 unspecified role unspecified role prey prey Stoichiometry: 2.0 N/A 1852625 unspecified role unspecified role prey prey Stoichiometry: 1.0 association association false false false Fig. 1B, Supp. Table 1 Trace amounts, as estimated by spectral count, of other proteins, mainly ribosomal and heat shock proteins were also identified but were not assumed by the authors to be part of the complex. Stoichiometry was estimated by spectral counting. hir1-hir2-4 1852621 N/A 1852622 unspecified role unspecified role neutral component neutral component N/A 1852623 unspecified role unspecified role neutral component neutral component N/A 1852624 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1852625 unspecified role unspecified role neutral component neutral component region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 1D The complex was first purified by Tap tag co-ip. The molecular weight of 670kDa confirmed the stoichiometry suggested by spectral counting.