IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hernandez-2004-3 rat-brain_pineal_gland rat-brain_pineal_gland pineal gland pineal gland anti bait coip anti bait coimmunoprecipitation anti bait coip tag visualisation tag visualisation Hernandez SC., Vicini S., Xiao Y., Dávila-García MI., Yasuda RP., Wolfe BB., Kellar KJ. Protein A (Pansorbin) or Protein G (Omnisorb) followed by centrifugation were used to separate antibody/ligand complexes. kellark@georgetown.edu Molecular pharmacology 2004 Only protein-protein interactions imex curation imex curation hernandez-2004-2 rat-brain_pineal_gland rat-brain_pineal_gland pineal gland pineal gland immunodepletion immunodepleted coimmunoprecipitation immunodepleted coip immunodepletion tag visualisation tag visualisation Hernandez SC., Vicini S., Xiao Y., Dávila-García MI., Yasuda RP., Wolfe BB., Kellar KJ. Rat pineal gland extract was mixed with [3H]epibatidine (a receptor ligand) and immunoprecipitated with either anti-alpha3 or anti-beta4 neuronal nicotinic acetylcholine receptor subunit antibodies, followed by a further round of immunoprecipitation of the supernatant with the reciprocal antibody. Precipitated subunits were visualised by scintillation counting. A reduction of the count after the second IP indicates an interaction between the two target proteins. kellark@georgetown.edu Molecular pharmacology 2004 Only protein-protein interactions imex curation imex curation hernandez-2004-4 rat-brain-cortex Rattus norvegicus brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] anti bait coip anti bait coimmunoprecipitation anti bait coip tag visualisation tag visualisation Hernandez SC., Vicini S., Xiao Y., Dávila-García MI., Yasuda RP., Wolfe BB., Kellar KJ. Protein A (Pansorbin) or Protein G (Omnisorb) followed by centrifugation were used to separate antibody/ligand complexes. kellark@georgetown.edu Molecular pharmacology 2004 Only protein-protein interactions imex curation imex curation epibatidine epibatidine (1R,2R,4S)-2-(6-chloropyridin-3-yl)-7-azabicyclo[2.2.1]heptane EPIBATIDINE Epibatidine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) acha3_rat Neuronal acetylcholine receptor subunit alpha-3 Chrna3 Acra3 protein protein rat Rattus norvegicus (Rat) Rat MGVVLLPPPLSMLMLVLMLLPAASASEAEHRLFQYLFEDYNEIIRPVANVSHPVIIQFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAIIKAPGYKHEIKYNCCEEIYQDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPTWVKAVFLNLLPRVMFMTRPTSGEGDTPKTRTFYGAELSNLNCFSRADSKSCKEGYPCQDGTCGYCHHRRVKISNFSANLTRSSSSESVNAVLSLSALSPEIKEAIQSVKYIAENMKAQNVAKEIQDDWKYVAMVIDRIFLWVFILVCILGTAGLFLQPLMARDDT achb4_rat Neuronal acetylcholine receptor subunit beta-4 Chrnb4 Acrb4 Neuronal acetylcholine receptor non-alpha-2 chain protein protein rat Rattus norvegicus (Rat) Rat MRGTPLLLVSLFSLLQDGDCRLANAEEKLMDDLLNKTRYNNLIRPATSSSQLISIRLELSLSQLISVNEREQIMTTSIWLKQEWTDYRLAWNSSCYEGVNILRIPAKRVWLPDIVLYNNADGTYEVSVYTNVIVRSNGSIQWLPPAIYKSACKIEVKHFPFDQQNCTLKFRSWTYDHTEIDMVLKSPTAIMDDFTPSGEWDIVALPGRRTVNPQDPSYVDVTYDFIIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTFFLLLISKIVPPTSLDIPLIGKYLLFTMVLVTFSIVTTVCVLNVHHRSPSTHTMASWVKECFLHKLPTFLFMKRPGLEVSLVRVPHPSQLHLATADTAATSALGPTSPSNLYGSSMYFVNPVPAAPKSAVSSHTAGLPRDARLRSSGRFREDLQEALEGVSFIAQHLESDDRDQSVIEDWKFVAMVVDRLFLWVFVFVCILGTMGLFLPPLFQIHAPSKDS achb2_rat Neuronal acetylcholine receptor subunit beta-2 Chrnb2 Acrb2 Neuronal acetylcholine receptor non-alpha-1 chain protein protein rat Rattus norvegicus (Rat) Rat MAGHSNSMALFSFSLLWLCSGVLGTDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISVHEREQIMTTNVWLTQEWEDYRLTWKPEDFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDLVLKSDVASLDDFTPSGEWDIIALPGRRNENPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMAPWVKVVFLEKLPTLLFLQQPRHRCARQRLRLRRRQREREGAGALFFREGPAADPCTCFVNPASVQGLAGAFRAEPTAAGPGRSVGPCSCGLREAVDGVRFIADHMRSEDDDQSVREDWKYVAMVIDRLFLWIFVFVCVFGTVGMFLQPLFQNYTATTFLHPDHSAPSSK chrna3-epibatidine-1 1790219 N/A 1790222 unspecified role unspecified role prey prey region 3h radiolabel 3h radiolabel 3H tritium H3 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1790223 unspecified role unspecified role bait bait physical association physical association false false false Fig. 8, 9a chrnb4-epibatidine-1 1790219 N/A 1790222 unspecified role unspecified role prey prey region 3h radiolabel 3h radiolabel 3H tritium H3 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1790224 unspecified role unspecified role bait bait physical association physical association false false false Fig. 8, 9b chrna3-chrnb4-2 1790220 N/A 1790222 unspecified role unspecified role unspecified role unspecified role region 3h radiolabel 3h radiolabel 3H tritium H3 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1790224 unspecified role unspecified role unspecified role unspecified role N/A 1790223 unspecified role unspecified role unspecified role unspecified role physical association physical association false false false Fig. 9a, 9b chrnb2-epibatidine-1 1790221 N/A 1790222 unspecified role unspecified role prey prey region 3h radiolabel 3h radiolabel 3H tritium H3 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1790225 unspecified role unspecified role bait bait physical association physical association false false false Fig. 8 (inset)