UniProt UniProt http://www.uniprot.org shimojo-2003-2 REST/NRSF-interacting LIM domain protein, a putative nuclear translocation receptor. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shimojo M., Hersh L.B. mshim1@uky.edu rat brain cDNA Mol. Cell. Biol. (0270-7306) 2003 imex curation Only protein-protein interactions shimojo-2003-1 REST/NRSF-interacting LIM domain protein, a putative nuclear translocation receptor. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Shimojo M., Hersh L.B. mshim1@uky.edu rat brain cDNA Mol. Cell. Biol. (0270-7306) 2003 imex curation Only protein-protein interactions rest_mouse RE1-silencing transcription factor Rest Nrsf Neural-restrictive silencer factor protein protein mouse Mus musculus Mouse MATQVMGQSSGGGSLFNNSANMGMALTNDMYDLHELSKAELAAPQLIMLANVALTGEASGSCCDYLVGEERQMAELMPVGDNHFSESEGEGLEESADLKGLENMELGSLELSAVEPQPVFEASAAPEIYSANKDPAPETPVAEDKCRSSKAKPFRCKPCQYEAESEEQFVHHIRIHSAKKFFVEESAEKQAKAWESGSSPAEEGEFSKGPIRCDRCGYNTNRYDHYMAHLKHHLRAGENERIYKCIICTYTTVSEYHWRKHLRNHFPRKVYTCSKCNYFSDRKNNYVQHVRTHTGERPYKCELCPYSSSQKTHLTRHMRTHSGEKPFKCDQCNYVASNQHEVTRHARQVHNGPKPLNCPHCDYKTADRSNFKKHVELHVNPRQFNCPVCDYAASKKCNLQYHFKSKHPTCPSKTMDVSKVKLKKTKKREADLLNNAVSNEKMENEQTKTKGDVSGKKNEKPVKAVGKDASKEKKPGSSVSVVQVTTRTRKSAVAAETKAAEVKHTDGQTGNNPEKPCKAKKNKRKKDAEAHPSEEPVNEGPVTKKKKKSECKSKIGTNVPKGGGRAEERPGVKKQSASLKKGTKKTPPKTKTSKKGGKLAPKGMGQTEPSSGALAQVGVSPDPALIQAEVTGSGSSQTELPSPMDIAKSEPAQMEVSLTGPPPVEPAQMEPSPAKPPQVEAPTYPQPPQRGPAPPTGPAPPTGPAPPTEPAPPTGLAEMEPSPTEPSQKEPPPSMEPPCPEELPQAEPPPMEDCQKELPSPVEPAQIEVAQTAPTQVQEEPPPVSEPPRVKPTKRSSLRKDRAEKELSLLSEMARQEQVLMGVGLVPVRDSKLLKGNKSAQDPPAPPSPSPKGNSREETPKDQEMVSDGEGTIVFPLKKGGPEEAGESPAELAALKESARVSSSEQNSAMPEGGASHSKCQTGSSGLCDVDTEQKTDTVPMKDSAAEPVSPPTPTVDRDAGSPAVVASPPITLAENESQEIDEDEGIHSHDGSDLSDNMSEGSDDSGLHGARPTPPEATSKNGKAGLAGKVTEGEFVCIFCDRSFRKEKDYSKHLNRHLVNVYFLEEAAEEQEEQEEREEQE pric1_human Prickle-like protein 1 PRICKLE1 RILP REST/NRSF-interacting LIM domain protein 1 protein protein human Homo sapiens Human MPLEMEPKMSKLAFGCQRSSTSDDDSGCALEEYAWVPPGLRPEQIQLYFACLPEEKVPYVNSPGEKHRIKQLLYQLPPHDNEVRYCQSLSEEEKKELQVFSAQRKKEALGRGTIKLLSRAVMHAVCEQCGLKINGGEVAVFASRAGPGVCWHPSCFVCFTCNELLVDLIYFYQDGKIHCGRHHAELLKPRCSACDEIIFADECTEAEGRHWHMKHFCCLECETVLGGQRYIMKDGRPFCCGCFESLYAEYCETCGEHIGVDHAQMTYDGQHWHATEACFSCAQCKASLLGCPFLPKQGQIYCSKTCSLGEDVHASDSSDSAFQSARSRDSRRSVRMGKSSRSADQCRQSLLLSPALNYKFPGLSGNADDTLSRKLDDLSLSRQGTSFASEEFWKGRVEQETPEDPEEWADHEDYMTQLLLKFGDKSLFQPQPNEMDIRASEHWISDNMVKSKTELKQNNQSLASKKYQSDMYWAQSQDGLGDSAYGSHPGPASSRRLQELELDHGASGYNHDETQWYEDSLECLSDLKPEQSVRDSMDSLALSNITGASVDGENKPRPSLYSLQNFEEMETEDCEKMSNMGTLNSSMLHRSAESLKSLSSELCPEKILPEEKPVHLPVLRRSKSQSRPQQVKFSDDVIDNGNYDIEIRQPPMSERTRRRVYNFEERGSRSHHHRRRRSRKSRSDNALNLVTERKYSPKDRLRLYTPDNYEKFIQNKSAREIQAYIQNADLYGQYAHATSDYGLQNPGMNRFLGLYGEDDDSWCSSSSSSSDSEEEGYFLGQPIPQPRPQRFAYYTDDLSSPPSALPTPQFGQRTTKSKKKKGHKGKNCIIS pric1_rat Prickle-like protein 1 Prickle1 Rilp REST/NRSF-interacting LIM domain protein 1 protein protein rat Rattus norvegicus (Rat) Rat MPLEMEPKMSKLAFGCQRSSTSDDDSGCALEEYAWVPPGLRPEQIQLYFACLPEEKVPYVNSPGEKHRIKQLLYQLPPHDNEVRYCQSLSEEEKKELQVFSAQRKKEALGRGTIKLLSRAMMHAVCEQCGLQMNGGEVAVFASRAGPGVCWRPSCFVCFTCNELLVDLIYFYQDGKIHCGRHHAELLKPRCSACDEIIFADECTEAEGRHWHMKHFCCLECETVLGGQRYIMKDGRPFCCGCFESLYAEYCETCGEHIGVDHAQMTYDGQHWHATEACFSCAQCKASLLGCPFLPKQGQIYCSKTCSLGEDIHASDSSDSAFQSARSRDSRRSVRMGRSSRSADQCRQSLLLSPALNYKFPGLSGSADDTLSRKLDDVSLSGQGAGFAHEEFWKARVDQEASEDPEEWAEHEDYMTQLLLKFGDKNLFQQPPSEVDMRASEHWIPDNMVTNKPEAKQNHQSLASKKYQSDMYWAQSQDGLGDSAYGSHPGPASSRRLQELDLDHGAAGYNHDQTQWYEDSLECLSDLKPEQSVRDSMDSLALSNITGASVDGESKPRPSLYSLQNFEEIEAEDCEKMSNMGTLNSSMLHRSAESLKSLNSELCPEKIIPEEKPVHLPVLRRSKSQSRPQQVKFSDDVIDNGSYDIEIRQPPMSERTRRRVYHFEERGSRPHHHRHRRSRKSRSDNALNLVTERKYSAKDRLRLYTPDNYEKFIQSKGARELQAYMQNANLYGQYAHTTSDYALQNPGMTRFLGLYGDDDDSWCSSSTSSSDSEEEGYFLGQPIPQPRPQRFTYYTDDLSSPASALPTPQFNQRTTKSKKKKGHRGKNCIIS rest-prickle1-3 1780224 n/a 1780226 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false znf_c2h2_2_5 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1780227 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 3A prickle1-rest-2 1780224 n/a 1780226 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection znf_c2h2_2_5 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1780227 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 3A rest-prickle1-1 1780225 n/a 1780228 unspecified role unspecified role prey prey n/a 1780226 unspecified role unspecified role bait bait znf_c2h2_2_5 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false no figure