IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ balestra-2000-1 Chick optic lobe contains a developmentally regulated alpha2alpha5beta2 nicotinic receptor subtype. chick-optic_lobe chicken optic lobe optic_lobe optic lobe anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Balestra B., Vailati S., Moretti M., Hanke W., Clementi F., Gotti C. Preys were detected with poly- and monoclonal antibodies against different parts of the proteins. The secondary antibodies were revealed with 125[I]-protein A. In cases where the secondary antibody was monoclonal, blots were incubated with anti-rat IgG for a further 2 hours prior to detection by 125[I]-protein A. gotti@csfic.mi.cnr.it Mol. Pharmacol. (0026-895X) 2000 Only protein-protein interactions imex curation balestra-2000-2 Chick optic lobe contains a developmentally regulated alpha2alpha5beta2 nicotinic receptor subtype. chick-retina Gallus gallus retina retinal tissue anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Balestra B., Vailati S., Moretti M., Hanke W., Clementi F., Gotti C. The secondary antibodies were revealed with 125[I]-protein A. In cases where the secondary antibody is monoclonal, blots were incubated with anti-rat IgG for a further 2 hours prior to development. gotti@csfic.mi.cnr.it Mol. Pharmacol. (0026-895X) 2000 Only protein-protein interactions imex curation acha4_chick Neuronal acetylcholine receptor subunit alpha-4 CHRNA4 protein protein chick Gallus gallus (Chicken) Chicken MGFLVSKGNLLLLLCASIFPAFGHVETRAHAEERLLKKLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDPQEYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFYDGRIKWMPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDLVSMHSHVDQLDYWESGEWVIINAVGNYNSKKYECCTEIYPDITYSFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSPRTHTMPDWVRRVFLDIVPRLLFMKRPSTVKDNCKKLIESMHKLTNSPRLWSETDMEPNFTTSSSPSPQSNEPSPTSSFCAHLEEPAKPMCKSPSGQYSMLHPEPPQVTCSSPKPSCHPLSDTQTTSISKGRSLSVQQMYSPNKTEEGSIRCRSRSIQYCYLQEDSSQTNGHSSASPASQRCHLNEEQPQHKPHQCKCKCRKGEAAGTPTQGSKSHSNKGEHLVLMSPALKLAVEGVHYIADHLRAEDADFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFLPPWLAGMI achb2_chick Neuronal acetylcholine receptor subunit beta-2 CHRNB2 Neuronal acetylcholine receptor non-alpha-1 chain protein protein chick Gallus gallus (Chicken) Chicken MALLRVLCLLAALRRSLCTDTEERLVEYLLDPTRYNKLIRPATNGSQLVTVQLMVSLAQLISVHEREQIMTTNVWLTQEWEDYRLTWKPEDFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVISYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDLVLKSEVASLDDFTPSGEWDIVALPGRRNENPDDSTYVDITYDFIIRRKPLFYTINLIIPCILITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMPPWVRTLFLRKLPALLFMKQPQQNCARQRLRQRRQTQERAAAATLFLRAGARACACYANPGAAKAEGLNGYRERQGQGPDPPAPCGCGLEEAVEGVRFIADHMRSEDDDQSVSEDWKYVAMVIDRLFLWIFVFVCVFGTVGMFLQPLFQNYATNSLLQLGQGTPTSK acha5_chick Neuronal acetylcholine receptor subunit alpha-5 CHRNA5 protein protein chick Gallus gallus (Chicken) Chicken MPLRARSRKPGAGPAARAPQAGVSEPSFVAKSEDRLFKHLFEDYQRWVRPVEHLNDTIKIKFGLAISQLVDVDEKNQLMTTNVWLKQEWIHVKLRWNPEDYAGITSIRVPSDSIWIPDIVLYDNADGRFEGTSTKTVVKYDGTIAWTPPVNYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDIILEDYEVDKRDFFDNGEWEIVTATGSKGNRTDGCCWYPFVTYSFIIRRLPLFYTLFLIIPCIGLSFLTVLVFYLPSNEAEKISLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIVITVFAINIHHRSSSTHNAMAPWVRKIFLHKLPKLLCMRSHVDRYFAQKEEKGNMSGSESSRNTLEAALDSIRYITRHVMKENEVREVVEDWKFIAQVLDRMFLWAFLLVSIIGSLVLFIPVIHKWASIIVPVHIGSTNT acha2_chick Neuronal acetylcholine receptor subunit alpha-2 CHRNA2 protein protein chick Gallus gallus (Chicken) Chicken MGWPCRSIIPLLVWCFVTLQAATREQKQPHGFAEDRLFKHLFTGYNRWSRPVPNTSDVVIVKFGLSIAQLIDVDEKNQMMTTNVWLKQEWSDYKLRWNPEDFDNVTSIRVPSEMIWIPDIVLYNNADGEFAVTHMTKAHLFSNGKVKWVPPAIYKSSCSIDVTYFPFDQQNCKMKFGSWTYDKAKIDLENMEHHVDLKDYWESGEWAIINAIGRYNSKKYDCCTEIYPDITFYFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSPSTHTMPHWVRSFFLGFIPRWLFMKRPPLLLPAEGTTGQYDPPGTRLSTSRCWLETDVDDKWEEEEEEEEEEEEEEEEEKAYPSRVPSGGSQGTQCHYSCERQAGKASGGPAPQVPLKGEEVGSDQGLTLSPSILRALEGVQYIADHLRAEDADFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFLPPYLAGMI achb4_chick Neuronal acetylcholine receptor subunit beta-4 CHRNB4 Neuronal acetylcholine receptor non-alpha-3 chain protein protein chick Gallus gallus (Chicken) Chicken STAADAEEKLMNHLLSPDRYNKLIRPAVNSSQLVSIELQVSLAQLISVNEREQIMTTNVWLNQEWIDYRLAWKPSDYEGINMLRIPAKHIWLPDIVLYNNADGTYEVSLYTNAIVQNNGSIRWLPPAIYKSACKIEVKHFPFDQQNCTLKFRSWTYDHTEIDMVLKTSMASMDDFTPSGEWDIVALPGRRTENPLDPNYVDVTYDFIIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLIGKYLMFTMVLVTFSIVTSVCVLNVHHRSPSTHTMPPWVKLVFLERLPAYLFMKRPENNSPRQKPANCKKTRAENLCMDPADFYKNSTYFVNTASAKKYDMKITDTLDNVSSHQDFRLRTGTKFSPEVQEAIDGVSFIAEHMKSDDNDQSVIEDWKYVAMVVDRLFLWIFVLVCVLGTVGLFLQPLFQNHIAATNP acha3_chick Neuronal acetylcholine receptor subunit alpha-3 CHRNA3 protein protein chick Gallus gallus (Chicken) Chicken MQSIHALLLTAAVCILFQGCGGSEPEHRLYAALFKNYNQFVRPVKNASDPVIIQFEVSMSQLVKVDEVNQIMETNLWLKHIWNDYKLRWNPVDYGGAEFIRVPSGQIWKPDIVLYNNAVGDFQVDDKTKALLKYTGDVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSTMNLKDYWESGEWAIIKAPGYKHDIKYNCCEEIYTDITYSLYIRRLPLFYTINMIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPKTHTMPVWVRTIFLNLLPRIMFMTRPTSDEENNQKPKPFYTSEFSNLNCFNSSEIKCCKDGFVCQDMACSCCQYQRMKFSDFSGNLTRSSSSESVDPLFSFSVLSPEMRDAIESVKYIAENMKMQNEAKEIQDDWKYVAMVIDRIFLWVFILVCILGTAGLFLQPLMTGDDM chrnb2-chrna4-8 1787664 n/a 1787666 unspecified role unspecified role prey prey n/a 1787667 unspecified role unspecified role bait bait physical association physical association false false false Fig. 3 (bottom) In Fig 3, the tissue extract was first immunodepleted of alpha3-, beta4- and alpha 5-containing receptors before it was IPed twice with beta2. chrnb2-chrna2-2 1787664 N/A 1787668 unspecified role unspecified role prey prey n/a 1787667 unspecified role unspecified role bait bait n/a 1787669 unspecified role unspecified role prey prey n/a 1787666 unspecified role unspecified role prey prey association association false false false Fig. 1 (top) chrna2-chrna5-1 1787664 n/a 1787668 unspecified role unspecified role prey prey n/a 1787669 unspecified role unspecified role bait bait n/a 1787667 unspecified role unspecified role prey prey association association false false false Fig. 3 (top) For this IP, the tissue extract was first immunodepleted of alpha3- and beta4-containing receptors before it was IPed twice with alpha2. chrnb4-chrna3-1 1787665 n/a 1787670 unspecified role unspecified role bait bait n/a 1787671 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1 (bottom)