IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ fretto-1993-2 Mechanism of platelet-derived growth factor (PDGF) AA, AB, and BB binding to alpha and beta PDGF receptor. in vitro In vitro competition binding competition binding tag visualisation tag visualisation Fretto L.J., Snape A.J., Tomlinson J.E., Seroogy J.J., Wolf D.L., LaRochelle W.J., Giese N.A. This is a Scachard Analysis in which the ratio of bound/free ligand is plotted against increasing concentrations of bound ligand. J. Biol. Chem. (0021-9258) 1993 Only protein-protein interactions imex curation fretto-1993-3 Mechanism of platelet-derived growth factor (PDGF) AA, AB, and BB binding to alpha and beta PDGF receptor. in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Fretto L.J., Snape A.J., Tomlinson J.E., Seroogy J.J., Wolf D.L., LaRochelle W.J., Giese N.A. J. Biol. Chem. (0021-9258) 1993 Only protein-protein interactions imex curation fretto-1993-4 Mechanism of platelet-derived growth factor (PDGF) AA, AB, and BB binding to alpha and beta PDGF receptor. in vitro In vitro electrophoresis electrophoretic mobility-based method electrophoresis predetermined predetermined participant predetermined Fretto L.J., Snape A.J., Tomlinson J.E., Seroogy J.J., Wolf D.L., LaRochelle W.J., Giese N.A. J. Biol. Chem. (0021-9258) 1993 Only protein-protein interactions imex curation fretto-1993-1 Mechanism of platelet-derived growth factor (PDGF) AA, AB, and BB binding to alpha and beta PDGF receptor. in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight silver stain molecular weight estimation by silver staining weight silver stain Fretto L.J., Snape A.J., Tomlinson J.E., Seroogy J.J., Wolf D.L., LaRochelle W.J., Giese N.A. J. Biol. Chem. (0021-9258) 1993 Only protein-protein interactions imex curation pdgfa_human Platelet-derived growth factor subunit A PDGFA PDGF1 Platelet-derived growth factor A chain Platelet-derived growth factor alpha polypeptide PDGF-1 protein protein human Homo sapiens Human MRTLACLLLLGCGYLAHVLAEEAEIPREVIERLARSQIHSIRDLQRLLEIDSVGSEDSLDTSLRAHGVHATKHVPEKRPLPIRRKRSIEEAVPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATTSLNPDYREEDTGRPRESGKKRKRKRLKPT pgfra_human Platelet-derived growth factor receptor alpha PDGFRA CD140 antigen-like family member A CD140a antigen Alpha-type platelet-derived growth factor receptor Alpha platelet-derived growth factor receptor Platelet-derived growth factor receptor 2 RHEPDGFRA PDGFR2 Platelet-derived growth factor alpha receptor protein protein human Homo sapiens Human MGTSHPAFLVLGCLLTGLSLILCQLSLPSILPNENEKVVQLNSSFSLRCFGESEVSWQYPMSEEESSDVEIRNEENNSGLFVTVLEVSSASAAHTGLYTCYYNHTQTEENELEGRHIYIYVPDPDVAFVPLGMTDYLVIVEDDDSAIIPCRTTDPETPVTLHNSEGVVPASYDSRQGFNGTFTVGPYICEATVKGKKFQTIPFNVYALKATSELDLEMEALKTVYKSGETIVVTCAVFNNEVVDLQWTYPGEVKGKGITMLEEIKVPSIKLVYTLTVPEATVKDSGDYECAARQATREVKEMKKVTISVHEKGFIEIKPTFSQLEAVNLHEVKHFVVEVRAYPPPRISWLKNNLTLIENLTEITTDVEKIQEIRYRSKLKLIRAKEEDSGHYTIVAQNEDAVKSYTFELLTQVPSSILDLVDDHHGSTGGQTVRCTAEGTPLPDIEWMICKDIKKCNNETSWTILANNVSNIITEIHSRDRSTVEGRVTFAKVEETIAVRCLAKNLLGAENRELKLVAPTLRSELTVAAAVLVLLVIVIISLIVLVVIWKQKPRYEIRWRVIESISPDGHEYIYVDPMQLPYDSRWEFPRDGLVLGRVLGSGAFGKVVEGTAYGLSRSQPVMKVAVKMLKPTARSSEKQALMSELKIMTHLGPHLNIVNLLGACTKSGPIYIITEYCFYGDLVNYLHKNRDSFLSHHPEKPKKELDIFGLNPADESTRSYVILSFENNGDYMDMKQADTTQYVPMLERKEVSKYSDIQRSLYDRPASYKKKSMLDSEVKNLLSDDNSEGLTLLDLLSFTYQVARGMEFLASKNCVHRDLAARNVLLAQGKIVKICDFGLARDIMHDSNYVSKGSTFLPVKWMAPESIFDNLYTTLSDVWSYGILLWEIFSLGGTPYPGMMVDSTFYNKIKSGYRMAKPDHATSEVYEIMVKCWNSEPEKRPSFYHLSEIVENLLPGQYKKSYEKIHLDFLKSDHPAVARMRVDSDNAYIGVTYKNEEDKLKDWEGGLDEQRLSADSGYIIPLPDIDPVPEEEDLGKRNRHSSQTSEESAIETGSSSSTFIKREDETIEDIDMMDDIGIDSSDLVEDSFL pdgfb_human Platelet-derived growth factor subunit B Platelet-derived growth factor B chain Platelet-derived growth factor beta polypeptide PDGF-2 Proto-oncogene c-Sis PDGFB PDGF2 SIS protein protein human Homo sapiens Human MNRCWALFLSLCCYLRLVSAEGDPIPEELYEMLSDHSIRSFDDLQRLLHGDPGEEDGAELDLNMTRSHSGGELESLARGRRSLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVTRSPGGSQEQRAKTPQTRVTIRTVRVRRPPKGKHRKFKHTHDKTALKETLGA pgfrb_human Platelet-derived growth factor receptor beta CD140 antigen-like family member B PDGFRB Beta-type platelet-derived growth factor receptor PDGFR Platelet-derived growth factor receptor 1 Beta platelet-derived growth factor receptor PDGFR1 protein protein human Homo sapiens Human MRLPGAMPALALKGELLLLSLLLLLEPQISQGLVVTPPGPELVLNVSSTFVLTCSGSAPVVWERMSQEPPQEMAKAQDGTFSSVLTLTNLTGLDTGEYFCTHNDSRGLETDERKRLYIFVPDPTVGFLPNDAEELFIFLTEITEITIPCRVTDPQLVVTLHEKKGDVALPVPYDHQRGFSGIFEDRSYICKTTIGDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGNEVVNFEWTYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGTLQFAELHRSRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNVSETRYVSELTLVRVKVAEAGHYTMRAFHEDAEVQLSFQLQINVPVRVLELSESHPDSGEQTVRCRGRGMPQPNIIWSACRDLKRCPRELPPTLLGNSSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQKKPRYEIRWKVIESVSSDGHEYIYVDPMQLPYDSTWELPRDQLVLGRTLGSGAFGQVVEATAHGLSHSQATMKVAVKMLKSTARSSEKQALMSELKIMSHLGPHLNVVNLLGACTKGGPIYIITEYCRYGDLVDYLHRNKHTFLQHHSDKRRPPSAELYSNALPVGLPLPSHVSLTGESDGGYMDMSKDESVDYVPMLDMKGDVKYADIESSNYMAPYDNYVPSAPERTCRATLINESPVLSYMDLVGFSYQVANGMEFLASKNCVHRDLAARNVLICEGKLVKICDFGLARDIMRDSNYISKGSTFLPLKWMAPESIFNSLYTTLSDVWSFGILLWEIFTLGGTPYPELPMNEQFYNAIKRGYRMAQPAHASDEIYEIMQKCWEEKFEIRPPFSQLVLLLERLLGEGYKKKYQQVDEEFLRSDHPAILRSQARLPGFHGLRSPLDTSSVLYTAVQPNEGDNDYIIPLPDPKPEVADEGPLEGSPSLASSTLNEVNTSSTISCDSPLEPQDEPEPEPQLELQVEPEPELEQLPDSGCPAPRAEAEDSFL pdgfra-pdgfa-4 1476586 n/a 1476590 unspecified role unspecified role prey prey region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa187 (aa 1-88 and 188-end deleted). n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Tag is attached to aa89 (aa 1-88 and 188-end deleted). ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1476591 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 1476601 1476597 direct interaction direct interaction false false false 1476586 Fig. 4a pdgfra-pdgfab 1476586 n/a 1476591 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. n/a 1476592 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1476590 unspecified role unspecified role prey prey c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa187 (aa 1-88 and 188-end deleted). n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Tag is attached to aa89 (aa 1-88 and 188-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 1476612 1476606 physical association physical association false false false 1476586 Fig. 4b pdgfra-pdgfb-6 1476586 n/a 1476591 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. n/a 1476592 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 1476619 1476616 direct interaction direct interaction false false false 1476586 Fig. 4c pdgfrb-pdgfab 1476586 n/a 1476593 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. n/a 1476592 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1476590 unspecified role unspecified role prey prey c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa187 (aa 1-88 and 188-end deleted). n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Tag is attached to aa89 (aa 1-88 and 188-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 1476631 1476626 physical association physical association false false false 1476586 Fig. 4d pdgfrb-pdgfb-1 1476586 n/a 1476592 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1476593 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 1476639 1476635 direct interaction direct interaction false false false 1476586 Fig. 4e pdgfra-pdgfb-8 1476586 n/a 1476592 competitor competitor competitor prey prey P01127:p.Arg108_Arg109del mutation mutation certain certain sequence position certain certain certain sequence position certain false pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1476592 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1476591 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 1476650 1476648 physical association physical association false false false Fig. 6 pdgfrb-pdgfb-3 1476586 n/a 1476592 competitor competitor competitor prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P01127:p.Arg108_Arg109del mutation mutation certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1476593 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. n/a 1476592 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 1476659 1476657 physical association physical association false false false Fig.6 pdgfrb-pdgfab-2 1476586 n/a 1476590 competitor competitor competitor prey prey c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa187 (aa 1-88 and 188-end deleted). n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Tag is attached to aa89 (aa 1-88 and 188-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1476592 competitor competitor competitor prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P01127:p.Arg108_Arg109del mutation mutation certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1476593 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. n/a 1476590 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa187 (aa 1-88 and 188-end deleted). n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Tag is attached to aa89 (aa 1-88 and 188-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1476592 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P01127:p.Arg108_Arg109del mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 1476668 1476666 1476680 1476674 physical association physical association false false false Fig. 8b pdgfra-pdgfb-9 1476586 n/a 1476591 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. n/a 1476592 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 6 pdgfrb-pdgfb-4 1476586 n/a 1476592 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1476593 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association false false false Fig.6 pdgfra-pdgfa-2 1476586 n/a 1476591 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. n/a 1476590 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Tag is attached to aa89 (aa 1-88 and 188-end deleted). c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa187 (aa 1-88 and 188-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1476590 competitor competitor competitor prey prey n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Tag is attached to aa89 (aa 1-88 and 188-end deleted). c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa187 (aa 1-88 and 188-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false 1476586 Fig. 4a pdgfra-pdgfa-2 1476586 n/a 1476590 competitor competitor competitor prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Tag is attached to aa89 (aa 1-88 and 188-end deleted). c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa187 (aa 1-88 and 188-end deleted). ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1476592 competitor competitor competitor prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1476591 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. n/a 1476592 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1476590 unspecified role unspecified role prey prey c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa187 (aa 1-88 and 188-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Tag is attached to aa89 (aa 1-88 and 188-end deleted). ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 physical association physical association false false false 1476586 Fig. 4b pdgfra-pdgfb-13 1476586 n/a 1476592 competitor competitor competitor prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1476592 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1476591 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. direct interaction direct interaction false false false 1476586 Fig. 4c pdgfrb-pdgfa-3 1476586 n/a 1476592 competitor competitor competitor prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1476593 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. n/a 1476590 competitor competitor competitor prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa187 (aa 1-88 and 188-end deleted). n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Tag is attached to aa89 (aa 1-88 and 188-end deleted). ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1476590 unspecified role unspecified role prey prey region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa187 (aa 1-88 and 188-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Tag is attached to aa89 (aa 1-88 and 188-end deleted). ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1476592 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 physical association physical association false false false 1476586 Fig. 4d pdgfrb-pdgfb-7 1476586 n/a 1476592 competitor competitor competitor prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1476593 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. n/a 1476592 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false 1476586 Fig. 4e pdgfrb-pdgfa-3 1476586 n/a 1476592 unspecified role unspecified role prey prey P01127:p.Arg108_Arg109del mutation mutation certain certain sequence position certain certain certain sequence position certain false region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1476590 unspecified role unspecified role prey prey c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa187 (aa 1-88 and 188-end deleted). n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Tag is attached to aa89 (aa 1-88 and 188-end deleted). region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1476593 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association false false false Fig. 8b pdgfra-pdgfab-1 1476587 n/a 1476591 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. n/a 1476592 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1476590 unspecified role unspecified role prey prey n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Tag is attached to aa89 (aa 1-88 and 188-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa187 (aa 1-88 and 188-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 1476790 1476784 physical association physical association false false false Fig. 5a, 8a The only difference between the two figures is that in Fig. 5a detection is with an anti-B chain antibody while in Fig. 8a detection is with an anti-PDGF chain antibody that detects A- and B-chains. pdgfra-pdgfb-7 1476587 n/a 1476592 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) n/a 1476591 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 1476796 1476793 physical association physical association false false false Fig. 5a pdgfrb-pdgfab-1 1476587 n/a 1476592 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1476590 unspecified role unspecified role prey prey c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa187 (aa 1-88 and 188-end deleted). n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Tag is attached to aa89 (aa 1-88 and 188-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1476593 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 1476805 1476801 1476807 1476799 physical association physical association false false false Fig. 5b pdgfrb-pdgfb-2 1476587 n/a 1476593 unspecified role unspecified role bait bait extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. n/a 1476592 unspecified role unspecified role prey prey pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) 1476812 1476810 physical association physical association false false false Fig. 5b pdgfa-4 1476588 n/a 1476590 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Tag is attached to aa89 (aa 1-88 and 188-end deleted). c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa187 (aa 1-88 and 188-end deleted). pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 1a pdgfb-3 1476588 n/a 1476592 unspecified role unspecified role neutral component neutral component pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 1a pdgfa-pdgfb 1476589 n/a 1476590 unspecified role unspecified role neutral component neutral component pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Tag is attached to aa89 (aa 1-88 and 188-end deleted). c-terminal kt3 epitope (tpppepet) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa187 (aa 1-88 and 188-end deleted). ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1476592 unspecified role unspecified role neutral component neutral component pdgf/vegf domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Tag is attached to aa182 (aa 1-82 and 183-end deleted). ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 1476829 1476825 direct interaction direct interaction false false false Fig. 1a, lane 5