MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint anonymous-2014p-1 The individual N-lobe and C-lobe of calmodulin tether to Cav1.2 channel and rescue the channel activity from run-down in ventricular myocytes of guinea-pig heart in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie lyhao@mail.cmu.edu.cn 2014 imex curation Shao D., Zhao M., Xu J., Feng R., Guo F., Hu H., Sun X., Gao Q., He G., Sun W., Wang H., Yu L., Liu S., Zhu Y., Etsuko M., Zhu T., Kameyama M., Hao L. FEBS Lett. (0014-5793) Only protein-protein interactions cac1c_cavpo Voltage-dependent L-type calcium channel subunit alpha-1C CACNA1C CACH2 CACN2 CACNL1A1 CCHL1A1 Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle Voltage-gated calcium channel subunit alpha Cav1.2 protein protein cavpo Cavia porcellus (Guinea pig) MVPLVQPTTPAYRPLPSHLSADTEVRGRGTLVHEAQLNCFYISPGGSNYGSPRPAHANINANAAAGLAPEHIPTPGAALSWQAAIDAGRQAKLMGSAGNTTISTVSSTQRKRQQYGKPKKQSGTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHTALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGITDVPAEEDPSPCALESGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGVDEEKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRLDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMRTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPTTKINMDDLQPNENEDKSPYPNPDAAGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDQPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPSMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESDPSNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVVMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTAMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDIGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFGRAGGLFGNHVSYYQSDSRSTFPQTFTTQRPLHINKAGNNQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRFPHPAGYPSTVSTVEGHRPPSSPATWAQEATRKLGAMRCHSRESQIAVVCQEEPSQDKTYDVELNKDAEYCSEPSLLSTEMLSYKDDENRQLTPPEEDKGDTRPSPKKGFLRSASLGRRASFHLECLKRQKNHGGDISQKTVLPLHLVHHQALAVAGLSPLLQRSHSPTAIPRPCATPPATPGSRGWPPKPIPTLRLEGAESCEKLNSSFPSIHCSSWSEEPSPCGGGSSAARRARPVSLMVPSQAGAPGRQFHGSASSLAEAVLISEGLGQFAQDPKFIEVTTQELADACDMTIGEMENAADNILSGGAPQSPNGTLLPFVNCRDPGQDRAGGDEDEGCACALGRGWSEEELADSRVHVRSL calm_human Calmodulin CALM1 CALM CAM CAM1 CALM2 CAMB CALM3 CAM3 CAMC CAMIII CAM2 CALML2 protein protein human Homo sapiens Human MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK calm1-q9z305 1567494 n/a Cav1.2 1567495 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a CaM C-lobe 1567496 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f2 t1 To further examine the Ca2+-dependence of C-lobe binding to the channel, we measured the interaction of CT1 with C-lobe34, an apo C-lobe mimic, at [Ca2+] of ≈ free to 2 mM. As shown in Fig. 2C, C-lobe34 was still able to bind to CT1 in a concentration-dependent manner, but the Ca2+-dependence was diminished. The Bmax was less than 1 mol/mol CaM/CT1 even in 2 mM Ca2+ (Fig. 2D). q9z305-calm1 1567494 n/a Cav1.2 1567495 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a CaM N-lobe 1567496 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f3 t1 To confirm that N-lobe may bind with CT1 at low Ca2+, we examined the binding of N-lobe12 with CT1. As shown in Fig. 3C, N-lobe12 bound to CT1 in a concentration-dependent manner but not in a Ca2+-dependent manner. The parameters obtained (Table 1) revealed that Kd values had no obvious Ca2+-dependence. The maximal binding was about 0.75 mol/mol N-lobe12/CT1 (Fig. 3D), supporting that N-lobe was capable to bind to the channel at low [Ca2+].