MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint anonymous-2014za-5 Structural basis for recognition of the type VI spike protein VgrG3 by a cognate immunity protein in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined dongcheng@ihep.ac.cn; dongyh@ihep.ac.cn 2014 imex curation Zhang J., Zhang H., Gao Z., Hu H., Dong C., Dong Y.H. FEBS Lett. (0014-5793) Only protein-protein interactions anonymous-2014za-2 Structural basis for recognition of the type VI spike protein VgrG3 by a cognate immunity protein in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined dongcheng@ihep.ac.cn; dongyh@ihep.ac.cn 2014 imex curation Zhang J., Zhang H., Gao Z., Hu H., Dong C., Dong Y.H. FEBS Lett. (0014-5793) Only protein-protein interactions anonymous-2014j-1 Structural basis for recognition of the type VI spike protein VgrG3 by a cognate immunity protein in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined dongcheng@ihep.ac.cn; dongyh@ihep.ac.cn 2014 imex curation Zhang J., Zhang H., Gao Z., Hu H., Dong C., Dong Y.H. FEBS Lett. (0014-5793) Only protein-protein interactions anonymous-2014za-3 Structural basis for recognition of the type VI spike protein VgrG3 by a cognate immunity protein in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined dongcheng@ihep.ac.cn; dongyh@ihep.ac.cn 2014 imex curation Zhang J., Zhang H., Gao Z., Hu H., Dong C., Dong Y.H. FEBS Lett. (0014-5793) Only protein-protein interactions anonymous-2014za-4 Structural basis for recognition of the type VI spike protein VgrG3 by a cognate immunity protein in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined dongcheng@ihep.ac.cn; dongyh@ihep.ac.cn 2014 imex curation Zhang J., Zhang H., Gao Z., Hu H., Dong C., Dong Y.H. FEBS Lett. (0014-5793) Only protein-protein interactions tsiv3_vibch Antitoxin protein TsiV3 tsiV3 VC_A0124 protein protein vibch Vibrio cholerae MNNLLSAYVTMLLILLSISGGAIASENCNDTSGVHQKILVCIQNEIAKSETQIRNNISSKSIDYGFPDDFYSKQRLAIHEKCMLYINVGGQRGELLMNQCELSMLQGLDIYIQQYIEDVDNS vgrg3_vibch Type VI secretion system spike protein VgrG3 vgrG3 Muramidase Vgrg3C VC_A0123 protein protein vibch Vibrio cholerae MARLQFQLKVDGLEDESLVVRGFEGQESLSDSVWRCEPCYGFRYQVDLASALSNLTAEQFVDQTAHLTILRDGQVVQQINGIVRQLSKGDTGHRHTFYSLTLVPALERLSLRSNSRIFQQQSVPEIISILLQEMGIEDYAFALKRECAQREFCVQYRETDLQFLHRIAAEEGLVYSHLHEAQKHTLLFTDSSDSQPKLAKPVPYNALAGGEINLPYVVDLQFKTTAQVSHTELKDYSFKKPAYGFTQRTQGKDIAYQQPNYEHFDAPGRYKDDANGKAFSQIRLEYLRRDALLADAKSDEPLLLAGVRFDLQDHLDHAMNRDWLVVQANHQGTQPQALQEEGGSGATTYSNQLKLIPAHITWRARPCAKPQVDGPMIATVVGPQGEEIYCDNFGRVKVHFPWDRYSSSNEKSSCWVRVAQEWAGSQYGSMAIPRVGHEVIVSFLNGDPDQPIITGRTYHATNTAPYALPDHKTKTVLRTETHQGQGYNELSFEDQAGSEQILLHAQKDWDALIEHDHTEVIRHDQHLTVDNDRFTRIQRNQHLTVEGEVRSKIALDSSHEVGASLQHKVGQRIAVEAGKEISLKSGAKIVVEAGAELTLKAGGSFVKVDAGGVHLVGPAINLNAGGSAGSGSAYGGQLAAAPRMLAQAKPVAELVQPDIAASMQSGAARVIDVASLPTMMPSSANNTANDEPVAEEKTPERILKSDLLKPSDELEKLAKRQASAYRQGNHSDEVKLLQEALIKLGFDLGKAGADGDFGSKTKTAIEQFQKSYQPSHQTHPSYSIGAVDGIVGKGTLLALDEALMDGWVYENNIYQIWPLGKTSEKYESAGRGPGVISTGNGDYGGASYGCYQMSSNLGVVQKYIQSSKFKEFFSGLNPATKEFNVVWQDIASRYPQEFREEQHQFIKRTHYDIQIGHLRGKGLLFEHNRAAVHDLIWSTSVQFGGRTNLIFNALNGQNMESMTDKDIIILVQDYKLVNTERLFKSSPSWWSDLKKRAVSEKKALLELEIDGLEVDIK q9kn41-q9kn42-6 1533982 n/a TsaB 1533987 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a VgrG3 1533988 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1533982 t2 q9kn41-q9kn42-7 1533982 n/a TsaB 1533987 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a VgrG3 1533988 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9KN42:p.Tyr826Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1533982 t2 q9kn41-q9kn42-8 1533982 n/a TsaB 1533987 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a VgrG3 1533988 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9KN42:p.Asp842Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1533982 t2 q9kn41-q9kn42-9 1533982 n/a VgrG3 1533988 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9KN42:p.Val941Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false n/a TsaB 1533987 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1533982 t2 q9kn41-q9kn42-10 1533982 n/a VgrG3 1533988 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified Q9KN42:p.Gln942Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a TsaB 1533987 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1533982 t2 q9kn41-q9kn42-11 1533982 n/a VgrG3 1533988 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9KN42:p.Leu982Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false n/a TsaB 1533987 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1533982 t2 q9kn41-q9kn42-12 1533982 n/a TsaB 1533987 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a VgrG3 1533988 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified Q9KN42:p.Arg996Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1533982 t2 q9kn41-q9kn42-1 1533983 n/a TsaB 1533987 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 n/a VgrG3 1533988 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false F1A q9kn41-q9kn42-3 1533983 n/a TsaB 1533987 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false F5A Although dimeric TsaB accounts for most of the total protein, higher oligomeric assemblies also occur in solution. The AUC and gel-filtration results are fully consistent (Figure 5A and Figure 2). q9kn41-q9kn41 1533983 n/a TsaB 1533987 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a TsaB 1533987 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false F5A Although dimeric TsaB accounts for most of the total protein, higher oligomeric assemblies also occur in solution. The AUC and gel-filtration results are fully consistent (Figure 5A and Figure 2). q9kn41-q9kn42 1533984 n/a TsaB 1533987 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a VgrG3 1533988 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false t1 F1C F6A F6B F6C q9kn41-q9kn42-2 1533984 n/a TsaB 1533987 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false F5B T1 The structure of native TsaB was determined at a resolution of 2.8 Å. There are six molecules in an asymmetric unit, which consists of three TsaB dimers (Figure 5B). q9kn41-4 1533985 n/a TsaB 1533987 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false F2 Although dimeric TsaB accounts for most of the total protein, higher oligomeric assemblies also occur in solution. The AUC and gel-filtration results are fully consistent (Figure 5A and Figure 2). q9kn41-q9kn42-4 1533985 n/a TsaB 1533987 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a VgrG3 1533988 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false F2 q9kn41-q9kn42-5 1533986 n/a TsaB 1533987 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 n/a VgrG3 1533988 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false F1b